UniProt Accession

 ACOX2_MOUSE; Q9QXD1; Q8VCB0 

Genbank Protein ID

 AJ238492; BC021339 

Genbank Nucleotide ID

 CAB65251.1; AAH21339.1 

Protein Name

 Peroxisomal acyl-coenzyme A oxidase 2 

Protein Synonyms/Alias

 3-alpha,7-alpha,12-alpha-trihydroxy-5-beta-cholestanoyl-CoA 24-hydroxylase; 3-alpha,7-alpha,12-alpha-trihydroxy-5-beta-cholestanoyl-CoA oxidase; Trihydroxycoprostanoyl-CoA oxidase; THCA-CoA oxidase; THCCox 

Gene Name

 Acox2 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
66	RDPVFNLKHLYFMTR	acetylation	[1]
66	RDPVFNLKHLYFMTR	ubiquitination	[2]
137	GSDEQIAKWGQLGKN	ubiquitination	[2]
244	TVGDIGPKMGFENID	ubiquitination	[2]
303	TRVQLLYKGFLPTLQ	acetylation	[3]
303	TRVQLLYKGFLPTLQ	succinylation	[3]
303	TRVQLLYKGFLPTLQ	ubiquitination	[2]
453	QVARFLMKSYLQAQV	acetylation	[3]
453	QVARFLMKSYLQAQV	succinylation	[3]
556	FLTVRNFKEAVEKLD	acetylation	[3]
556	FLTVRNFKEAVEKLD	succinylation	[3]
556	FLTVRNFKEAVEKLD	ubiquitination	[2]
561	NFKEAVEKLDNEPEI	acetylation	[3]
561	NFKEAVEKLDNEPEI	succinylation	[3]
655	RLFEWAQKSPANTQE	acetylation	[1, 4]
667	TQENPAYKKYIRPLM	acetylation	[3, 5]
667	TQENPAYKKYIRPLM	succinylation	[3]
678	RPLMQSWKPKL****	acetylation	[1]
680	LMQSWKPKL******	acetylation	[1]

Reference

 [1] Calorie restriction and SIRT3 trigger global reprogramming of the mitochondrial protein acetylome.
 Hebert AS, Dittenhafer-Reed KE, Yu W, Bailey DJ, Selen ES, Boersma MD, Carson JJ, Tonelli M, Balloon AJ, Higbee AJ, Westphall MS, Pagliarini DJ, Prolla TA, Assadi-Porter F, Roy S, Denu JM, Coon JJ.
 Mol Cell. 2013 Jan 10;49(1):186-99. [PMID: 23201123]
 [2] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [3] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337]
 [4] Quantification of mitochondrial acetylation dynamics highlights prominent sites of metabolic regulation.
 Still AJ, Floyd BJ, Hebert AS, Bingman CA, Carson JJ, Gunderson DR, Dolan BK, Grimsrud PA, Dittenhafer-Reed KE, Stapleton DS, Keller MP, Westphall MS, Denu JM, Attie AD, Coon JJ, Pagliarini DJ.
 J Biol Chem. 2013 Jul 17;. [PMID: 23864654]
 [5] Substrate and functional diversity of lysine acetylation revealed by a proteomics survey.
 Kim SC, Sprung R, Chen Y, Xu Y, Ball H, Pei J, Cheng T, Kho Y, Xiao H, Xiao L, Grishin NV, White M, Yang XJ, Zhao Y.
 Mol Cell. 2006 Aug;23(4):607-18. [PMID: 16916647] 

Functional Description

 Oxidizes the CoA esters of the bile acid intermediates di- and tri-hydroxycoprostanic acids (By similarity). 

Sequence Annotation

 MOTIF 679 681 Microbody targeting signal (Potential).
 MOD_RES 667 667 N6-acetyllysine.  

Keyword

 Acetylation; Complete proteome; FAD; Fatty acid metabolism; Flavoprotein; Lipid metabolism; Oxidoreductase; Peroxisome; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 681 AA 

Protein Sequence

Gene Ontology

 GO:0005777; C:peroxisome; IEA:UniProtKB-SubCell.
 GO:0033791; F:3alpha,7alpha,12alpha-trihydroxy-5beta-cholestanoyl-CoA 24-hydroxylase activity; IEA:EC.
 GO:0003995; F:acyl-CoA dehydrogenase activity; IEA:InterPro.
 GO:0003997; F:acyl-CoA oxidase activity; IEA:InterPro.
 GO:0050660; F:flavin adenine dinucleotide binding; IEA:InterPro.
 GO:0006635; P:fatty acid beta-oxidation; IEA:InterPro. 

Interpro

 IPR006091; Acyl-CoA_Oxase/DH_cen-dom.
 IPR012258; Acyl-CoA_oxidase.
 IPR002655; Acyl-CoA_oxidase_C.
 IPR009075; AcylCo_DH/oxidase_C.
 IPR013786; AcylCoA_DH/ox_N.
 IPR009100; AcylCoA_DH/oxidase. 

Pfam

 PF01756; ACOX 

SMART

  

PROSITE

  

PRINTS