CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-014937
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 GRAM domain-containing protein 4 
Protein Synonyms/Alias
 Death-inducing protein 
Gene Name
 GRAMD4 
Gene Synonyms/Alias
 DIP; KIAA0767 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
127RRMELEQKVQEVLKAubiquitination[1]
133QKVQEVLKARTEEQMubiquitination[1]
168GLSSRLQKWFYERFGubiquitination[2, 3]
385RCPRLRAKYDTPYIIubiquitination[1]
403LPTDPQLKERSSAAVubiquitination[1]
432SAPAGLGKEEDAGRFubiquitination[1, 4]
523ITDIQKYKVLSVLPGubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [4] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302] 
Functional Description
 Plays a role as a mediator of E2F1-induced apoptosis in the absence of p53/TP53. 
Sequence Annotation
 DOMAIN 445 523 GRAM.
 MOD_RES 24 24 Phosphoserine.
 MOD_RES 28 28 Phosphoserine.  
Keyword
 Alternative splicing; Apoptosis; Coiled coil; Complete proteome; Membrane; Mitochondrion; Phosphoprotein; Polymorphism; Reference proteome; Transmembrane; Transmembrane helix. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 578 AA 
Protein Sequence
MLRRLDKIRF RGHKRDDFLD LAESPNASDT ECSDEIPLKV PRTSPRDSEE LRDPAGPGTL 60
IMATGVQDFN RTEFDRLNEI KGHLEIALLE KHFLQEELRK LREETNAEML RQELDRERQR 120
RMELEQKVQE VLKARTEEQM AQQPPKGQAQ ASNGAERRSQ GLSSRLQKWF YERFGEYVED 180
FRFQPEENTV ETEEPLSARR LTENMRRLKR GAKPVTNFVK NLSALSDWYS VYTSAIAFTV 240
YMNAVWHGWA IPLFLFLAIL RLSLNYLIAR GWRIQWSIVP EVSEPVEPPK EDLTVSEKFQ 300
LVLDVAQKAQ NLFGKMADIL EKIKNLFMWV QPEITQKLYV ALWAAFLASC FFPYRLVGLA 360
VGLYAGIKFF LIDFIFKRCP RLRAKYDTPY IIWRSLPTDP QLKERSSAAV SRRLQTTSSR 420
SYVPSAPAGL GKEEDAGRFH STKKGNFHEI FNLTENERPL AVCENGWRCC LINRDRKMPT 480
DYIRNGVLYV TENYLCFESS KSGSSKRNKV IKLVDITDIQ KYKVLSVLPG SGMGIAVSTP 540
STQKPLVFGA MVHRDEAFET ILSQYIKITS AAASGGDS 578 
Gene Ontology
 GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
 GO:0031966; C:mitochondrial membrane; IEA:UniProtKB-SubCell.
 GO:0006915; P:apoptotic process; IEA:UniProtKB-KW. 
Interpro
 IPR004182; GRAM. 
Pfam
 PF02893; GRAM 
SMART
 SM00568; GRAM 
PROSITE
  
PRINTS