CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-029574
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 N-alpha-acetyltransferase 50 
Protein Synonyms/Alias
 cDNA FLJ51877, highly similar to Homo sapiens Mak3 homolog (MAK3), mRNA; cDNA, FLJ79525, highly similar to Homo sapiens Mak3 homolog (MAK3), mRNA 
Gene Name
 NAA50 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
17RRLGIGTKMLNHVLNubiquitination[1, 2, 3]
52SAIDFYRKFGFEIIEubiquitination[4]
61GFEIIETKKNYYKRIubiquitination[5]
78ADAHVLQKNLKVPSGubiquitination[3, 4, 6]
81HVLQKNLKVPSGQNAubiquitination[3]
92GQNADVQKTDN****ubiquitination[5, 6]
Reference
 [1] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [2] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
 Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
 J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931]
 [3] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [4] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [5] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [6] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 95 AA 
Protein Sequence
MTLGCLAPYR RLGIGTKMLN HVLNICEKDG TFDNIYLHVQ ISNESAIDFY RKFGFEIIET 60
KKNYYKRIEP ADAHVLQKNL KVPSGQNADV QKTDN 95 
Gene Ontology
 GO:0008080; F:N-acetyltransferase activity; IEA:InterPro. 
Interpro
 IPR016181; Acyl_CoA_acyltransferase.
 IPR000182; GNAT_dom. 
Pfam
 PF00583; Acetyltransf_1 
SMART
  
PROSITE
 PS51186; GNAT 
PRINTS