CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-016705
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Thimet oligopeptidase 
Protein Synonyms/Alias
  
Gene Name
 Thop1 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
2******MKPPAACAGacetylation[1]
45TQLIEQTKCVYDRVGacetylation[1]
92PQHVSPCKDIRAASTubiquitination[2]
128RVVWLQEKTPKNSLKacetylation[1]
147RYLERLIKLGRRNGLacetylation[1]
220GKLKVTLKYPHYFPLacetylation[1]
229PHYFPLLKKCHVPETacetylation[1]
257EENCAILKELVSLRAacetylation[1]
303DELAQKLKPLGEQERacetylation[1]
421DLYPREGKYGHAACFacetylation[1]
538APQDLLEKLIKSRQAubiquitination[2]
628DMFHTRFKQEGVLSPacetylation[1]
636QEGVLSPKVGMDYRTubiquitination[2]
667QFLGRDPKQDAFLLSacetylation[1]
667QFLGRDPKQDAFLLSubiquitination[2]
Reference
 [1] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
 [2] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 
Functional Description
 Involved in the metabolism of neuropeptides under 20 amino acid residues long. Involved in cytoplasmic peptide degradation. Able to degrade the beta-amyloid precursor protein and generate amyloidogenic fragments (By similarity). 
Sequence Annotation
 ACT_SITE 474 474 By similarity.
 METAL 473 473 Zinc; catalytic (By similarity).
 METAL 477 477 Zinc; catalytic (By similarity).
 METAL 480 480 Zinc; catalytic (By similarity).
 MOD_RES 257 257 N6-acetyllysine (By similarity).
 MOD_RES 278 278 Phosphotyrosine.
 MOD_RES 538 538 N6-acetyllysine (By similarity).  
Keyword
 Acetylation; Complete proteome; Cytoplasm; Hydrolase; Metal-binding; Metalloprotease; Phosphoprotein; Protease; Reference proteome; Zinc. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 687 AA 
Protein Sequence
MKPPAACAGD VVDAASPAST VNHLRWDLSA QQIRALTTQL IEQTKCVYDR VGAQNFEDVS 60
YESTLKALAD VEVTYTVQRN ILDFPQHVSP CKDIRAASTE ADKKLSEFDV EMSMRQDVYQ 120
RVVWLQEKTP KNSLKPEAAR YLERLIKLGR RNGLHLPQDT QEKIKNIKKR LSLLCIDFNK 180
NLNEDTTFLP FTREELGGLP EDFLSSLEKA EDGKLKVTLK YPHYFPLLKK CHVPETRRLL 240
EEAFNCRCKE ENCAILKELV SLRAQKSSLL GFHTHADYVL EMNMAKTSQT VATFLDELAQ 300
KLKPLGEQER AVILELKEAE CAKRGLPFDG RIHAWDMRYY MNQVEETRYR VDQNLLKEYF 360
PMQVVTRGLL AIYQELLGLT FTLEEGAAAW HEDVRLYSVR DAASGEEIGK FYLDLYPREG 420
KYGHAACFGL QPGCLRQDGS RQLAVAAMVA NFTKPTPDAP SLLQHDEVET YFHEFGHVMH 480
QLCSQAEFAM FSGTHVERDF VEAPSQMLEN WVWEKEPLMR MSQHYRTGSE APQDLLEKLI 540
KSRQANAGLF NLRQIVLAKV DQVLHTQTDA DPAEEYARLC QEILGVPATP GTNMPATFGH 600
LAGGYDAQYY GYLWSEVYSM DMFHTRFKQE GVLSPKVGMD YRTSILRPGG SEDASAMLKQ 660
FLGRDPKQDA FLLSKGLQVE GSEAPAC 687 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0004222; F:metalloendopeptidase activity; IDA:MGI.
 GO:0042277; F:peptide binding; IEA:Compara.
 GO:0007243; P:intracellular protein kinase cascade; IDA:MGI.
 GO:0006518; P:peptide metabolic process; IDA:MGI.
 GO:0006508; P:proteolysis; IEA:UniProtKB-KW. 
Interpro
 IPR024079; MetalloPept_cat_dom.
 IPR024077; Neurolysin/TOP_dom2.
 IPR024080; Neurolysin/TOP_N.
 IPR001567; Pept_M3A_M3B. 
Pfam
 PF01432; Peptidase_M3 
SMART
  
PROSITE
 PS00142; ZINC_PROTEASE 
PRINTS