UniProt Accession

 CGL_RAT; P18757 

Genbank Protein ID

 D17370; X53460 

Genbank Nucleotide ID

 BAA04189.1; CAA37547.1 

Protein Name

 Cystathionine gamma-lyase 

Protein Synonyms/Alias

 Cysteine-protein sulfhydrase; Gamma-cystathionase; Probasin-related antigen; PRB-RA 

Gene Name

 Cth 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Rattus norvegicus (Rat) 

NCBI Taxa ID

 10116 

Lysine Modification

Position	Peptide	Type	References
140	FVDCSKTKLLEAAIT	acetylation	[1]
164	TPTNPTLKLADIKAC	acetylation	[1]
259	YLCCRGLKTLQIRME	acetylation	[1]
287	ESNPRVEKVIYPGLP	acetylation	[1]
303	HPQHELAKRQCTGCP	acetylation	[1]
329	QHAQVFLKNIKLFAL	acetylation	[1]
383	SVGLEDEKDLLEDLG	acetylation	[1]
394	EDLGQALKAAHP***	acetylation	[1]

Reference

 [1] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns.
 Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV.
 Cell Rep. 2012 Aug 30;2(2):419-31. [PMID: 22902405] 

Functional Description

 Catalyzes the last step in the trans-sulfuration pathway from methionine to cysteine. Has broad substrate specificity. Converts cystathionine to cysteine, ammonia and 2-oxobutanoate. Converts two cysteine molecules to lanthionine and hydrogen sulfide. Can also accept homocysteine as substrate. Specificity depends on the levels of the endogenous substrates. Generates the endogenous signaling molecule hydrogen sulfide (H2S), and so contributes to the regulation of blood pressure. Acts as a cysteine-protein sulfhydrase by mediating sulfhydration of target proteins: sulfhydration consists of converting -SH groups into -SSH on specific cysteine residues of target proteins such as GAPDH, PTPN1 and NF-kappa-B subunit RELA, thereby regulating their function (By similarity). 

Sequence Annotation

 BINDING 61 61 Substrate (By similarity).
 BINDING 113 113 Substrate (By similarity).
 BINDING 118 118 Substrate (By similarity).
 BINDING 338 338 Substrate (By similarity).
 MOD_RES 211 211 N6-(pyridoxal phosphate)lysine.
 MOD_RES 376 376 Phosphoserine (By similarity).  

Keyword

 Amino-acid biosynthesis; Calmodulin-binding; Complete proteome; Cysteine biosynthesis; Cytoplasm; Direct protein sequencing; Lyase; Phosphoprotein; Pyridoxal phosphate; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 398 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0004121; F:cystathionine beta-lyase activity; IDA:RGD.
 GO:0004123; F:cystathionine gamma-lyase activity; ISS:UniProtKB.
 GO:0080146; F:L-cysteine desulfhydrase activity; IEA:EC.
 GO:0044540; F:L-cystine L-cysteine-lyase (deaminating); ISS:UniProtKB.
 GO:0030170; F:pyridoxal phosphate binding; ISS:UniProtKB.
 GO:0019344; P:cysteine biosynthetic process; ISS:UniProtKB.
 GO:0006749; P:glutathione metabolic process; IMP:RGD.
 GO:0070814; P:hydrogen sulfide biosynthetic process; ISS:UniProtKB.
 GO:0043066; P:negative regulation of apoptotic process; ISS:UniProtKB.
 GO:0030308; P:negative regulation of cell growth; IMP:RGD.
 GO:0008285; P:negative regulation of cell proliferation; IMP:RGD.
 GO:0043123; P:positive regulation of I-kappaB kinase/NF-kappaB cascade; ISS:UniProtKB.
 GO:0051092; P:positive regulation of NF-kappaB transcription factor activity; ISS:UniProtKB.
 GO:0044524; P:protein sulfhydration; ISS:UniProtKB.
 GO:0018272; P:protein-pyridoxal-5-phosphate linkage via peptidyl-N6-pyridoxal phosphate-L-lysine; ISS:UniProtKB.
 GO:0019346; P:transsulfuration; TAS:RGD. 

Interpro

 IPR000277; Cys/Met-Metab_PyrdxlP-dep_enz.
 IPR015424; PyrdxlP-dep_Trfase.
 IPR015421; PyrdxlP-dep_Trfase_major_sub1.
 IPR015422; PyrdxlP-dep_Trfase_major_sub2. 

Pfam

 PF01053; Cys_Met_Meta_PP 

SMART

  

PROSITE

 PS00868; CYS_MET_METAB_PP 

PRINTS