CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-018444
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Solute carrier family 12 member 5 
Protein Synonyms/Alias
 Electroneutral potassium-chloride cotransporter 2; K-Cl cotransporter 2; mKCC2; Neuronal K-Cl cotransporter 
Gene Name
 Slc12a5 
Gene Synonyms/Alias
 Kcc2; Kiaa1176 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
59INSTDTEKGREYDGRubiquitination[1]
388LWSSYLTKGVIVERRubiquitination[1]
691EEGPPHTKNWRPQLLubiquitination[1]
895DDNSIQMKKDLTTFLubiquitination[1]
896DNSIQMKKDLTTFLYubiquitination[1]
940EQRSQILKQMHLTKNubiquitination[1]
1041KSVAEKNKGPSPVSSubiquitination[1]
1052PVSSEGIKDFFSMKPubiquitination[1]
1058IKDFFSMKPEWENLNubiquitination[1]
1085LNEVIVNKSRDAKLVubiquitination[1]
1090VNKSRDAKLVLLNMPubiquitination[1]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 
Functional Description
 Mediates electroneutral potassium-chloride cotransport in mature neurons. Transport occurs under isotonic conditions, but is activated 20-fold by cell swelling. Important for Cl(-) homeostasis in neurons. 
Sequence Annotation
 CARBOHYD 442 442 N-linked (GlcNAc...) (Potential).
 CARBOHYD 833 833 N-linked (GlcNAc...) (Potential).  
Keyword
 Alternative splicing; Cell membrane; Cell projection; Complete proteome; Direct protein sequencing; Glycoprotein; Ion transport; Membrane; Potassium; Potassium transport; Reference proteome; Symport; Transmembrane; Transmembrane helix; Transport. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1138 AA 
Protein Sequence
MSRRFTVTSL PPAASAASAD PESRRHSVAD PRRLPREDVK GDGNPKESSP FINSTDTEKG 60
REYDGRNMAL FEEEMDTSPM VSSLLSGLAN YTNLPQGSRE HEEAENNEGG KKKPVQAPRM 120
GTFMGVYLPC LQNIFGVILF LRLTWVVGIA GIMESFCMVF ICCSCTMLTA ISMSAIATNG 180
VVPAGGSYYM ISRSLGPEFG GAVGLCFYLG TTFAGAMYIL GTIEILLAYL FPAMAIFKAE 240
DASGEAAAML NNMRVYGTCV LTCMATVVFV GVKYVNKFAL VFLGCVILSI LAIYAGVIKS 300
AFDPPNFPIC LLGNRTLSRH GFDVCAKLAW EGNETVTTRL WGLFCSSRLL NATCDEYFTR 360
NNVTEIQGIP GAASGLIKEN LWSSYLTKGV IVERRGMPSV GLADGTPVDM DHPYVFSDMT 420
SYFTLLVGIY FPSVTGIMAG SNRSGDLRDA QKSIPTGTIL AIATTSAVYI SSVVLFGACI 480
EGVVLRDKFG EAVNGNLVVG TLAWPSPWVI VIGSFFSTCG AGLQSLTGAP RLLQAISRDG 540
IVPFLQVFGH GKANGEPTWA LLLTACICEI GILIASLDEV APILSMFFLM CYMFVNLACA 600
VQTLLRTPNW RPRFRYYHWT LSFLGMSLCL ALMFICSWYY ALVAMLIAGL IYKYIEYRGA 660
EKEWGDGIRG LSLSAARYAL LRLEEGPPHT KNWRPQLLVL VRVDQDQNVV HPQLLSLTSQ 720
LKAGKGLTIV GSVLEGTFLD NHPQAQRAEE SIRRLMEAEK VKGFCQVVIS SNLRDGVSHL 780
IQSGGLGGLQ HNTVLVGWPR NWRQKEDHQT WRNFIELVRE TTAGHLALLV TKNVSMFPGN 840
PERFSEGSID VWWIVHDGGM LMLLPFLLRH HKVWRKCKMR IFTVAQMDDN SIQMKKDLTT 900
FLYHLRITAE VEVVEMHESD ISAYTYEKTL VMEQRSQILK QMHLTKNERE REIQSITDES 960
RGSIRRKNPA NPRLRLNVPE ETACDNEEKP EEEVQLIHDQ SAPSCPSSSP SPGEEPEGER 1020
ETDPEVHLTW TKDKSVAEKN KGPSPVSSEG IKDFFSMKPE WENLNQSNVR RMHTAVRLNE 1080
VIVNKSRDAK LVLLNMPGPP RNRNGDENYM EFLEVLTEQL DRVMLVRGGG REVITIYS 1138 
Gene Ontology
 GO:0030425; C:dendrite; IEA:UniProtKB-SubCell.
 GO:0043198; C:dendritic shaft; IDA:MGI.
 GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
 GO:0043025; C:neuronal cell body; IDA:MGI.
 GO:0005886; C:plasma membrane; IDA:MGI.
 GO:0015379; F:potassium:chloride symporter activity; IEA:InterPro.
 GO:0007612; P:learning; IMP:MGI.
 GO:0035264; P:multicellular organism growth; IMP:MGI.
 GO:0006813; P:potassium ion transport; IEA:UniProtKB-KW.
 GO:0042493; P:response to drug; IMP:MGI.
 GO:0007268; P:synaptic transmission; IMP:MGI.
 GO:0040040; P:thermosensory behavior; IMP:MGI. 
Interpro
 IPR004841; AA-permease_dom.
 IPR000076; KCL_cotranspt.
 IPR004842; Na/K/Cl_cotransptS. 
Pfam
 PF00324; AA_permease 
SMART
  
PROSITE
  
PRINTS
 PR01081; KCLTRNSPORT.