CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-023861
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Ribosomal biogenesis protein LAS1L 
Protein Synonyms/Alias
 Protein LAS1 homolog 
Gene Name
 LAS1L 
Gene Synonyms/Alias
 MSTP060 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
31YGKCVKGKGSLPLSAubiquitination[1, 2]
64YLFCDDHKLQRYALNubiquitination[1, 3]
98TADLIRCKLLDVTGGubiquitination[1, 3, 4]
135ERKTKFAKVPLKCLAubiquitination[1, 2]
139KFAKVPLKCLAQEVNubiquitination[1, 2, 3, 5, 6]
162RHELTHKKMPHINDCubiquitination[1, 2]
215EEDQEEDKNIVVDDIubiquitination[5]
226VDDITEQKPEPQDDGubiquitination[5]
241KSTESDVKADGDSKGubiquitination[5]
257EEVDSHCKKALSHKEubiquitination[5]
258EVDSHCKKALSHKELubiquitination[1]
263CKKALSHKELYERARubiquitination[1, 2, 3, 5, 6, 7, 8]
286EQFTVLEKFRYLPKAubiquitination[3, 5]
292EKFRYLPKAIKAWNNubiquitination[2]
295RYLPKAIKAWNNPSPubiquitination[1, 2, 3, 4, 5]
312ECVLAELKGVTCENRubiquitination[1, 2, 3, 4, 5, 6]
365GTDPKSHKNVDLNDVubiquitination[1, 7, 8]
376LNDVLVPKPFSQFWQubiquitination[1]
432ELIVANTKTGRNARRubiquitination[5]
486QLLRIIFKAMGQGLPubiquitination[1, 2, 3, 4, 5, 6, 7, 8, 9]
499LPDEEQEKLLRICSIubiquitination[1, 2, 3, 4, 5, 7, 8]
540DSLYWSVKPASSSFGubiquitination[1, 2, 3, 4, 5, 6, 9]
565QGSVNDVKEEEKEEKubiquitination[5]
691RLEPSTCKTDTLGLSubiquitination[1, 3]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [3] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [4] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [5] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [6] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
 Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
 J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572]
 [7] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [8] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [9] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661] 
Functional Description
 Involved in the biogenesis of the 60S ribosomal subunit. Required for maturation of the 28S rRNA. Functions as a component of the Five Friends of Methylated CHTOP (5FMC) complex; the 5FMC complex is recruited to ZNF148 by methylated CHTOP, leading to desumoylation of ZNF148 and subsequent transactivation of ZNF148 target genes. 
Sequence Annotation
 MOD_RES 523 523 Phosphoserine.
 MOD_RES 560 560 Phosphoserine.
 MOD_RES 617 617 Phosphoserine.  
Keyword
 Alternative splicing; Complete proteome; Cytoplasm; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; rRNA processing. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 734 AA 
Protein Sequence
MSWESGAGPG LGSQGMDLVW SAWYGKCVKG KGSLPLSAHG IVVAWLSRAE WDQVTVYLFC 60
DDHKLQRYAL NRITVWRSRS GNELPLAVAS TADLIRCKLL DVTGGLGTDE LRLLYGMALV 120
RFVNLISERK TKFAKVPLKC LAQEVNIPDW IVDLRHELTH KKMPHINDCR RGCYFVLDWL 180
QKTYWCRQLE NSLRETWELE EFREGIEEED QEEDKNIVVD DITEQKPEPQ DDGKSTESDV 240
KADGDSKGSE EVDSHCKKAL SHKELYERAR ELLVSYEEEQ FTVLEKFRYL PKAIKAWNNP 300
SPRVECVLAE LKGVTCENRE AVLDAFLDDG FLVPTFEQLA ALQIEYEDGQ TEVQRGEGTD 360
PKSHKNVDLN DVLVPKPFSQ FWQPLLRGLH SQNFTQALLE RMLSELPALG ISGIRPTYIL 420
RWTVELIVAN TKTGRNARRF SAGQWEARRG WRLFNCSASL DWPRMVESCL GSPCWASPQL 480
LRIIFKAMGQ GLPDEEQEKL LRICSIYTQS GENSLVQEGS EASPIGKSPY TLDSLYWSVK 540
PASSSFGSEA KAQQQEEQGS VNDVKEEEKE EKEVLPDQVE EEEENDDQEE EEEDEDDEDD 600
EEEDRMEVGP FSTGQESPTA ENARLLAQKR GALQGSAWQV SSEDVRWDTF PLGRMPGQTE 660
DPAELMLENY DTMYLLDQPV LEQRLEPSTC KTDTLGLSCG VGSGNCSNSS SSNFEGLLWS 720
QGQLHGLKTG LQLF 734 
Gene Ontology
 GO:0005737; C:cytoplasm; IDA:HPA.
 GO:0071339; C:MLL1 complex; IDA:UniProtKB.
 GO:0006364; P:rRNA processing; IEA:UniProtKB-KW. 
Interpro
 IPR007174; Las1. 
Pfam
 PF04031; Las1 
SMART
  
PROSITE
  
PRINTS