UniProt Accession

 M1IP1_HUMAN; Q9NPA3; D3DWB2 

Genbank Protein ID

 AJ272057; AK001248; CR457220; CH471141; CH471141; BC008908; BC019332; BK001260 

Genbank Nucleotide ID

 CAB89113.1; BAA91580.1; CAG33501.1; EAW59435.1; EAW59436.1; AAH08908.1; AAH19332.1; DAA01482.1 

Protein Name

 Mid1-interacting protein 1 

Protein Synonyms/Alias

 Gastrulation-specific G12-like protein; Mid1-interacting G12-like protein; Protein STRAIT11499; Spot 14-related protein; S14R; Spot 14-R 

Gene Name

 MID1IP1 

Gene Synonyms/Alias

 MIG12 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
11	ICDTYNQKHSLFNAM	ubiquitination	[1, 2, 3]
95	YSHYVLLKSIRNDIE	ubiquitination	[2, 4]
122	SEEGSAWKSKDILVD	ubiquitination	[2, 4, 5, 6]
124	EGSAWKSKDILVDLG	ubiquitination	[2, 4, 5]
160	GLHTVLSKLTRKANI	ubiquitination	[2]
164	VLSKLTRKANILTNR	ubiquitination	[2, 3]
173	NILTNRYKQEIGFGN	ubiquitination	[1, 2, 3]

Reference

 [1] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [4] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [5] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [6] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094] 

Functional Description

 Plays a role in the regulation of lipogenesis in liver. Up-regulates ACACA enzyme activity. Required for efficient lipid biosynthesis, including triacylglycerol, diacylglycerol and phospholipid. Involved in stabilization of microtubules (By similarity). 

Sequence Annotation

 MOD_RES 1 1 N-acetylmethionine.
 MOD_RES 75 75 Phosphoserine (By similarity).  

Keyword

 Acetylation; Complete proteome; Cytoplasm; Cytoskeleton; Lipid biosynthesis; Lipid metabolism; Microtubule; Nucleus; Phosphoprotein; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 183 AA 

Protein Sequence

Gene Ontology

 GO:0005829; C:cytosol; ISS:UniProtKB.
 GO:0005874; C:microtubule; IEA:UniProtKB-KW.
 GO:0015630; C:microtubule cytoskeleton; ISS:UniProtKB.
 GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
 GO:0006629; P:lipid metabolic process; IEA:UniProtKB-KW.
 GO:0007026; P:negative regulation of microtubule depolymerization; ISS:UniProtKB.
 GO:0045723; P:positive regulation of fatty acid biosynthetic process; ISS:UniProtKB.
 GO:0051351; P:positive regulation of ligase activity; ISS:UniProtKB.
 GO:0051258; P:protein polymerization; ISS:UniProtKB. 

Interpro

 IPR009786; Spot_14. 

Pfam

 PF07084; Spot_14 

SMART

  

PROSITE

  

PRINTS