CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-015718
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Nuclear cap-binding protein subunit 1 
Protein Synonyms/Alias
 80 kDa nuclear cap-binding protein; CBP80; NCBP 80 kDa subunit 
Gene Name
 Cbp80 
Gene Synonyms/Alias
 CG7035 
Created Date
 July 27, 2013 
Organism
 Drosophila melanogaster (Fruit fly) 
NCBI Taxa ID
 7227 
Lysine Modification
Position
Peptide
Type
References
708VVERMEEKLEAANVNacetylation[1]
Reference
 [1] Proteome-wide mapping of the Drosophila acetylome demonstrates a high degree of conservation of lysine acetylation.
 Weinert BT, Wagner SA, Horn H, Henriksen P, Liu WR, Olsen JV, Jensen LJ, Choudhary C.
 Sci Signal. 2011 Jul 26;4(183):ra48. [PMID: 21791702] 
Functional Description
 Component of the cap-binding complex (CBC), which binds cotranscriptionally to the 5'-cap of pre-mRNAs and is involved in various processes such as pre-mRNA splicing and RNA-mediated gene silencing (RNAi). The CBC complex is involved in miRNA-mediated RNA interference via its interaction with Ars2 and is required for primary microRNAs (miRNAs) processing. Also involved in innate immunity via the short interfering RNAs (siRNAs) processing machinery by restricting the viral RNA production. In the CBC complex, Cbp80 does not bind directly capped RNAs (m7GpppG-capped RNA) but is required to stabilize the movement of the N-terminal loop of Cbp20 and lock the CBC into a high affinity cap-binding state with the cap structure. 
Sequence Annotation
 DOMAIN 31 243 MIF4G.
 MOD_RES 9 9 Phosphothreonine.  
Keyword
 Complete proteome; mRNA capping; mRNA processing; mRNA splicing; Nucleus; Phosphoprotein; Reference proteome; RNA-mediated gene silencing. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 800 AA 
Protein Sequence
MSRRRAHDTE DEGYDHRRNK RRRVSENQEI EDRLESLILR VGERSTSSVE SNLEGLVSVL 60
EADLGTFRLK ILRILSDCAV RMPEKCTVYT TLVGLLNAKN YKFGGEFVDH MVKTFKESLK 120
MCRWDAARYS LRFLADLVNC HVISATSLLQ LLDTMIDVSN EDTVPQVRRD WFVFAVLSTL 180
PWVGRDLYEK KESALESLLL RIEVYLNKRS KKHHNALRVW SSDAPHPQEE YLDCLWAQIR 240
KLRQDNWAEK HIPRPYLVFD SILCEALQHN LPTIVPPPHH DNFEYPMPWV VYRMFDYTDC 300
PDGPNLPGAH SIERFLIEEH LHHIIETYHH ERKDCAAQLL SFPYKHKIPL EYCIVEVVFA 360
ELFHMPTPRY LDICYGSILI ELCKLQPATL PQVLAQATEI LFMRIDSMNT SCFDRFVNWF 420
SYHLSNFKFT WSWDEWDSCL LLDGEHPRPK FIQEVLQKCL RLSYHQRITE MMPTTYAKLI 480
PLTPVPNYKY ANEEAANLPG TTVAHQLVVA IRQKCTPEEV VNILKDIPNS GYSGEEMSDG 540
SFNALKIDVF VQTLLNLGSK SFSHSFAAIS KFHSVFRALA ETEEAQICIL HNIFELWSSH 600
QQMMVVLIDK LLKLQIVDCS AVATWIFSKE MTGEFTKLYL WEILHLTIKK MNKHVIKLNT 660
ELSEAKEKLA KADSSSSDSE DDSSHKRKKP ITHADKPSEE VVERMEEKLE AANVNQKRLF 720
LIVFQRFIMI LSEHLLRSDT DGRDPDTDWY RWTIGRLQQV FLMHHEQVQK YSSTLETLLF 780
TSDLDTHILE VFQQFVALRA 800 
Gene Ontology
 GO:0071013; C:catalytic step 2 spliceosome; IDA:FlyBase.
 GO:0005846; C:nuclear cap binding complex; IEA:InterPro.
 GO:0071011; C:precatalytic spliceosome; IDA:FlyBase.
 GO:0003677; F:DNA binding; IEA:InterPro.
 GO:0000339; F:RNA cap binding; ISS:FlyBase.
 GO:0006370; P:7-methylguanosine mRNA capping; IEA:UniProtKB-KW.
 GO:0045292; P:mRNA cis splicing, via spliceosome; IEA:InterPro.
 GO:0000398; P:mRNA splicing, via spliceosome; ISS:FlyBase.
 GO:0051028; P:mRNA transport; IEA:InterPro.
 GO:0045071; P:negative regulation of viral genome replication; IMP:FlyBase.
 GO:0022008; P:neurogenesis; IMP:FlyBase.
 GO:0031053; P:primary miRNA processing; IMP:UniProtKB.
 GO:0030422; P:production of siRNA involved in RNA interference; IMP:FlyBase. 
Interpro
 IPR016024; ARM-type_fold.
 IPR027159; CBP80.
 IPR016021; MIF4-like_typ_1/2/3.
 IPR015172; MIF4G-like_typ-1.
 IPR015174; MIF4G-like_typ-2.
 IPR003890; MIF4G-like_typ-3. 
Pfam
 PF02854; MIF4G
 PF09088; MIF4G_like
 PF09090; MIF4G_like_2 
SMART
 SM00543; MIF4G 
PROSITE
  
PRINTS