CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-022010
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Histidine triad nucleotide-binding protein 3 
Protein Synonyms/Alias
 HINT-3 
Gene Name
 HINT3 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
78LICFKDIKPAATHHYubiquitination[1, 2, 3, 4, 5]
115ENMVTVGKTILERNNubiquitination[1, 2, 4, 6, 7]
179TADHLIEKLRT****ubiquitination[1, 3, 4, 5, 7, 8, 9, 10]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [4] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [5] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [6] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
 Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
 J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931]
 [7] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [8] Mass spectrometric analysis of lysine ubiquitylation reveals promiscuity at site level.
 Danielsen JM, Sylvestersen KB, Bekker-Jensen S, Szklarczyk D, Poulsen JW, Horn H, Jensen LJ, Mailand N, Nielsen ML.
 Mol Cell Proteomics. 2011 Mar;10(3):M110.003590. [PMID: 21139048]
 [9] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [10] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965
Functional Description
 Hydrolyzes phosphoramidate and acyl-adenylate substrates. 
Sequence Annotation
 DOMAIN 49 160 HIT.
 NP_BIND 76 77 Purine nucleotide phosphoramidate (By
 NP_BIND 145 147 Purine nucleotide phosphoramidate (By
 MOTIF 143 147 Histidine triad motif.
 ACT_SITE 145 145 Tele-AMP-histidine intermediate.
 MOD_RES 2 2 N-acetylalanine.  
Keyword
 Acetylation; Complete proteome; Cytoplasm; Hydrolase; Nucleotide-binding; Nucleus; Polymorphism; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 182 AA 
Protein Sequence
MAEEQVNRSA GLAPDCEASA TAETTVSSVG TCEAAGKSPE PKDYDSTCVF CRIAGRQDPG 60
TELLHCENED LICFKDIKPA ATHHYLVVPK KHIGNCRTLR KDQVELVENM VTVGKTILER 120
NNFTDFTNVR MGFHMPPFCS ISHLHLHVLA PVDQLGFLSK LVYRVNSYWF ITADHLIEKL 180
RT 182 
Gene Ontology
 GO:0005739; C:mitochondrion; IDA:HPA.
 GO:0005730; C:nucleolus; IDA:HPA.
 GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
 GO:0000166; F:nucleotide binding; IEA:UniProtKB-KW.
 GO:0008152; P:metabolic process; IEA:GOC. 
Interpro
 IPR001310; Histidine_triad_HIT.
 IPR011146; HIT-like. 
Pfam
  
SMART
  
PROSITE
 PS00892; HIT_1
 PS51084; HIT_2 
PRINTS