UniProt Accession

 ACADV_RAT; P45953 

Genbank Protein ID

 D30647 

Genbank Nucleotide ID

 BAA06331.1 

Protein Name

 Very long-chain specific acyl-CoA dehydrogenase, mitochondrial 

Protein Synonyms/Alias

 VLCAD 

Gene Name

 Acadvl 

Gene Synonyms/Alias

 Vlcad 

Created Date

 July 27, 2013 

Organism

 Rattus norvegicus (Rat) 

NCBI Taxa ID

 10116 

Lysine Modification

Position	Peptide	Type	References
71	KPARAESKSFAVGMF	acetylation	[1]
121	EEVNDPAKNDSLEKV	acetylation	[1]
127	AKNDSLEKVEEDTLQ	acetylation	[1]
195	GILLYGTKAQKEKYL	acetylation	[1]
200	GTKAQKEKYLPRVAS	acetylation	[1]
239	AVPSPCGKYYTLNGS	acetylation	[1]
268	VFAKTPIKDAATGAV	ubiquitination	[2]
276	DAATGAVKEKITAFV	acetylation	[1]
278	ATGAVKEKITAFVVE	acetylation	[1]
298	VTHGLPEKKMGIKAS	acetylation	[1]
316	EVYFDGVKVPAENVL	acetylation	[1]
331	GEVGDGFKVAVNILN	acetylation	[1]
358	TMKAIIAKAVDHATN	acetylation	[1]
372	NRTQFGDKIHNFGVI	acetylation	[1]
382	NFGVIQEKLARMAIL	acetylation	[1]
550	FATVVEAKLMKHKKG	acetylation	[1]
556	AKLMKHKKGIVNEQF	acetylation	[1]
639	RNFRSISKAMVENGG	acetylation	[1]

Reference

 [1] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns.
 Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV.
 Cell Rep. 2012 Aug 30;2(2):419-31. [PMID: 22902405]
 [2] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 

Functional Description

 Active toward esters of long-chain and very long chain fatty acids such as palmitoyl-CoA, mysritoyl-CoA and stearoyl-CoA. Can accommodate substrate acyl chain lengths as long as 24 carbons, but shows little activity for substrates of less than 12 carbons. 

Sequence Annotation

 NP_BIND 214 223 FAD (By similarity).
 NP_BIND 249 251 FAD (By similarity).
 NP_BIND 435 439 FAD (By similarity).
 NP_BIND 464 466 FAD (By similarity).
 REGION 41 482 Catalytic.
 REGION 338 341 Substrate binding (By similarity).
 REGION 462 463 Substrate binding (By similarity).
 REGION 483 516 Membrane-anchoring (Probable).
 ACT_SITE 462 462 Proton acceptor (By similarity).
 BINDING 223 223 Substrate; via carbonyl oxygen (By
 BINDING 366 366 FAD (By similarity).
 BINDING 463 463 Substrate; via amide nitrogen (By
 MOD_RES 237 237 S-nitrosocysteine (By similarity).
 MOD_RES 239 239 N6-acetyllysine (By similarity).
 MOD_RES 276 276 N6-acetyllysine (By similarity).
 MOD_RES 331 331 N6-acetyllysine; alternate (By
 CROSSLNK 331 331 Glycyl lysine isopeptide (Lys-Gly)  

Keyword

 Acetylation; Complete proteome; Direct protein sequencing; FAD; Fatty acid metabolism; Flavoprotein; Isopeptide bond; Lipid metabolism; Membrane; Mitochondrion; Mitochondrion inner membrane; Oxidoreductase; Reference proteome; S-nitrosylation; Transit peptide; Ubl conjugation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 655 AA 

Protein Sequence

Gene Ontology

 GO:0005743; C:mitochondrial inner membrane; IDA:RGD.
 GO:0000062; F:fatty-acyl-CoA binding; IDA:RGD.
 GO:0050660; F:flavin adenine dinucleotide binding; IDA:RGD.
 GO:0017099; F:very-long-chain-acyl-CoA dehydrogenase activity; IDA:RGD.
 GO:0033539; P:fatty acid beta-oxidation using acyl-CoA dehydrogenase; IMP:RGD.
 GO:0042760; P:very long-chain fatty acid catabolic process; IMP:RGD. 

Interpro

 IPR006089; Acyl-CoA_DH_CS.
 IPR006092; Acyl-CoA_DH_N.
 IPR006090; Acyl-CoA_Oxase/DH_1.
 IPR006091; Acyl-CoA_Oxase/DH_cen-dom.
 IPR009075; AcylCo_DH/oxidase_C.
 IPR013786; AcylCoA_DH/ox_N.
 IPR009100; AcylCoA_DH/oxidase. 

Pfam

 PF00441; Acyl-CoA_dh_1
 PF02770; Acyl-CoA_dh_M
 PF02771; Acyl-CoA_dh_N 

SMART

  

PROSITE

 PS00072; ACYL_COA_DH_1
 PS00073; ACYL_COA_DH_2 

PRINTS