CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-004960
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Multidrug resistance protein 1A 
Protein Synonyms/Alias
 ATP-binding cassette sub-family B member 1A; MDR1A; Multidrug resistance protein 3; P-glycoprotein 3 
Gene Name
 Abcb1a 
Gene Synonyms/Alias
 Abcb4; Mdr1a; Mdr3; Pgy-3; Pgy3 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
363GAAYEVFKIIDNKPSubiquitination[1]
407RKEVQILKGLNLKVKacetylation[2]
412ILKGLNLKVKSGQTVacetylation[2]
501DEIEKAVKEANAYDFubiquitination[1]
511NAYDFIMKLPHQFDTubiquitination[1]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [2] Calorie restriction and SIRT3 trigger global reprogramming of the mitochondrial protein acetylome.
 Hebert AS, Dittenhafer-Reed KE, Yu W, Bailey DJ, Selen ES, Boersma MD, Carson JJ, Tonelli M, Balloon AJ, Higbee AJ, Westphall MS, Pagliarini DJ, Prolla TA, Assadi-Porter F, Roy S, Denu JM, Coon JJ.
 Mol Cell. 2013 Jan 10;49(1):186-99. [PMID: 23201123] 
Functional Description
 Energy-dependent efflux pump responsible for decreased drug accumulation in multidrug-resistant cells. 
Sequence Annotation
 DOMAIN 50 353 ABC transmembrane type-1 1.
 DOMAIN 388 624 ABC transporter 1.
 DOMAIN 707 996 ABC transmembrane type-1 2.
 DOMAIN 1031 1269 ABC transporter 2.
 NP_BIND 423 430 ATP 1 (By similarity).
 NP_BIND 1066 1073 ATP 2 (By similarity).
 MOD_RES 667 667 Phosphoserine.
 CARBOHYD 83 83 N-linked (GlcNAc...) (Potential).
 CARBOHYD 87 87 N-linked (GlcNAc...) (Potential).
 CARBOHYD 90 90 N-linked (GlcNAc...) (Potential).  
Keyword
 3D-structure; ATP-binding; Cell membrane; Complete proteome; Direct protein sequencing; Glycoprotein; Hydrolase; Membrane; Nucleotide-binding; Phosphoprotein; Reference proteome; Repeat; Transmembrane; Transmembrane helix; Transport. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1276 AA 
Protein Sequence
MELEEDLKGR ADKNFSKMGK KSKKEKKEKK PAVSVLTMFR YAGWLDRLYM LVGTLAAIIH 60
GVALPLMMLI FGDMTDSFAS VGNVSKNSTN MSEADKRAMF AKLEEEMTTY AYYYTGIGAG 120
VLIVAYIQVS FWCLAAGRQI HKIRQKFFHA IMNQEIGWFD VHDVGELNTR LTDDVSKINE 180
GIGDKIGMFF QAMATFFGGF IIGFTRGWKL TLVILAISPV LGLSAGIWAK ILSSFTDKEL 240
HAYAKAGAVA EEVLAAIRTV IAFGGQKKEL ERYNNNLEEA KRLGIKKAIT ANISMGAAFL 300
LIYASYALAF WYGTSLVISK EYSIGQVLTV FFSVLIGAFS VGQASPNIEA FANARGAAYE 360
VFKIIDNKPS IDSFSKSGHK PDNIQGNLEF KNIHFSYPSR KEVQILKGLN LKVKSGQTVA 420
LVGNSGCGKS TTVQLMQRLY DPLDGMVSID GQDIRTINVR YLREIIGVVS QEPVLFATTI 480
AENIRYGRED VTMDEIEKAV KEANAYDFIM KLPHQFDTLV GERGAQLSGG QKQRIAIARA 540
LVRNPKILLL DEATSALDTE SEAVVQAALD KAREGRTTIV IAHRLSTVRN ADVIAGFDGG 600
VIVEQGNHDE LMREKGIYFK LVMTQTAGNE IELGNEACKS KDEIDNLDMS SKDSGSSLIR 660
RRSTRKSICG PHDQDRKLST KEALDEDVPP ASFWRILKLN STEWPYFVVG IFCAIINGGL 720
QPAFSVIFSK VVGVFTNGGP PETQRQNSNL FSLLFLILGI ISFITFFLQG FTFGKAGEIL 780
TKRLRYMVFK SMLRQDVSWF DDPKNTTGAL TTRLANDAAQ VKGATGSRLA VIFQNIANLG 840
TGIIISLIYG WQLTLLLLAI VPIIAIAGVV EMKMLSGQAL KDKKELEGSG KIATEAIENF 900
RTVVSLTREQ KFETMYAQSL QIPYRNAMKK AHVFGITFSF TQAMMYFSYA ACFRFGAYLV 960
TQQLMTFENV LLVFSAIVFG AMAVGQVSSF APDYAKATVS ASHIIRIIEK TPEIDSYSTQ 1020
GLKPNMLEGN VQFSGVVFNY PTRPSIPVLQ GLSLEVKKGQ TLALVGSSGC GKSTVVQLLE 1080
RFYDPMAGSV FLDGKEIKQL NVQWLRAQLG IVSQEPILFD CSIAENIAYG DNSRVVSYEE 1140
IVRAAKEANI HQFIDSLPDK YNTRVGDKGT QLSGGQKQRI AIARALVRQP HILLLDEATS 1200
ALDTESEKVV QEALDKAREG RTCIVIAHRL STIQNADLIV VIQNGKVKEH GTHQQLLAQK 1260
GIYFSMVSVQ AGAKRS 1276 
Gene Ontology
 GO:0016324; C:apical plasma membrane; IBA:RefGenome.
 GO:0000139; C:Golgi membrane; IBA:RefGenome.
 GO:0016021; C:integral to membrane; IBA:RefGenome.
 GO:0046581; C:intercellular canaliculus; IDA:MGI.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0042626; F:ATPase activity, coupled to transmembrane movement of substances; IBA:RefGenome.
 GO:0008559; F:xenobiotic-transporting ATPase activity; IEA:EC.
 GO:0007623; P:circadian rhythm; IEA:Compara.
 GO:0015893; P:drug transport; IDA:MGI.
 GO:0007595; P:lactation; IEA:Compara.
 GO:0060548; P:negative regulation of cell death; IEA:Compara.
 GO:0046686; P:response to cadmium ion; IEA:Compara.
 GO:0001666; P:response to hypoxia; IEA:Compara.
 GO:0033280; P:response to vitamin D; IEA:Compara. 
Interpro
 IPR003593; AAA+_ATPase.
 IPR003439; ABC_transporter-like.
 IPR017871; ABC_transporter_CS.
 IPR017940; ABC_transporter_type1.
 IPR001140; ABC_transptr_TM_dom.
 IPR011527; ABC_transptrTM_dom_typ1.
 IPR027417; P-loop_NTPase. 
Pfam
 PF00664; ABC_membrane
 PF00005; ABC_tran 
SMART
 SM00382; AAA 
PROSITE
 PS50929; ABC_TM1F
 PS00211; ABC_TRANSPORTER_1
 PS50893; ABC_TRANSPORTER_2 
PRINTS