CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-000093
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Myc box-dependent-interacting protein 1 
Protein Synonyms/Alias
 Amphiphysin II; Amphiphysin-like protein; Box-dependent myc-interacting protein 1; Bridging integrator 1 
Gene Name
 BIN1 
Gene Synonyms/Alias
 AMPHL 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
146RIAKRGRKLVDYDSAubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 
Functional Description
 May be involved in regulation of synaptic vesicle endocytosis. May act as a tumor suppressor and inhibits malignant cell transformation. 
Sequence Annotation
 DOMAIN 29 276 BAR.
 DOMAIN 520 592 SH3.
 REGION 1 122 Interaction with BIN2.
 REGION 378 421 Clathrin-binding.
 MOD_RES 296 296 Phosphoserine.
 MOD_RES 298 298 Phosphoserine.
 MOD_RES 303 303 Phosphoserine.
 MOD_RES 307 307 Phosphothreonine.
 MOD_RES 323 323 Phosphothreonine.
 MOD_RES 331 331 Phosphoserine.  
Keyword
 3D-structure; Alternative splicing; Coiled coil; Complete proteome; Cytoplasm; Developmental protein; Differentiation; Disease mutation; Endocytosis; Host-virus interaction; Nucleus; Phosphoprotein; Reference proteome; SH3 domain; Tumor suppressor. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 593 AA 
Protein Sequence
MAEMGSKGVT AGKIASNVQK KLTRAQEKVL QKLGKADETK DEQFEQCVQN FNKQLTEGTR 60
LQKDLRTYLA SVKAMHEASK KLNECLQEVY EPDWPGRDEA NKIAENNDLL WMDYHQKLVD 120
QALLTMDTYL GQFPDIKSRI AKRGRKLVDY DSARHHYESL QTAKKKDEAK IAKPVSLLEK 180
AAPQWCQGKL QAHLVAQTNL LRNQAEEELI KAQKVFEEMN VDLQEELPSL WNSRVGFYVN 240
TFQSIAGLEE NFHKEMSKLN QNLNDVLVGL EKQHGSNTFT VKAQPSDNAP AKGNKSPSPP 300
DGSPAATPEI RVNHEPEPAG GATPGATLPK SPSQLRKGPP VPPPPKHTPS KEVKQEQILS 360
LFEDTFVPEI SVTTPSQFEA PGPFSEQASL LDLDFDPLPP VTSPVKAPTP SGQSIPWDLW 420
EPTESPAGSL PSGEPSAAEG TFAVSWPSQT AEPGPAQPAE ASEVAGGTQP AAGAQEPGET 480
AASEAASSSL PAVVVETFPA TVNGTVEGGS GAGRLDLPPG FMFKVQAQHD YTATDTDELQ 540
LKAGDVVLVI PFQNPEEQDE GWLMGVKESD WNQHKELEKC RGVFPENFTE RVP 593 
Gene Ontology
 GO:0015629; C:actin cytoskeleton; TAS:ProtInc.
 GO:0043679; C:axon terminus; IEA:Compara.
 GO:0044300; C:cerebellar mossy fiber; IEA:Compara.
 GO:0005737; C:cytoplasm; IDA:LIFEdb.
 GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
 GO:0008021; C:synaptic vesicle; IEA:Compara.
 GO:0043196; C:varicosity; IEA:Compara.
 GO:0008283; P:cell proliferation; TAS:ProtInc.
 GO:0006897; P:endocytosis; IEA:UniProtKB-KW.
 GO:0007275; P:multicellular organismal development; IEA:UniProtKB-KW.
 GO:0042692; P:muscle cell differentiation; IEA:Compara.
 GO:0045807; P:positive regulation of endocytosis; IEA:Compara.
 GO:0043547; P:positive regulation of GTPase activity; IEA:Compara.
 GO:0019048; P:virus-host interaction; IEA:UniProtKB-KW. 
Interpro
 IPR027267; AH/BAR-dom.
 IPR003005; Amphiphysin.
 IPR003023; Amphiphysin_2.
 IPR004148; BAR_dom.
 IPR011511; SH3_2.
 IPR001452; SH3_domain. 
Pfam
 PF03114; BAR
 PF07653; SH3_2 
SMART
 SM00721; BAR
 SM00326; SH3 
PROSITE
 PS51021; BAR
 PS50002; SH3 
PRINTS
 PR01251; AMPHIPHYSIN.
 PR01253; AMPHIPHYSIN2.
 PR00452; SH3DOMAIN.