CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-002523
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Beta-hexosaminidase subunit beta 
Protein Synonyms/Alias
 Beta-N-acetylhexosaminidase subunit beta; Hexosaminidase subunit B; Cervical cancer proto-oncogene 7 protein; HCC-7; N-acetyl-beta-glucosaminidase subunit beta; Beta-hexosaminidase subunit beta chain B; Beta-hexosaminidase subunit beta chain A 
Gene Name
 HEXB 
Gene Synonyms/Alias
 HCC7 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
51RAPSVSAKPGPALWPubiquitination[1]
161KEPVAVLKANRVWGAubiquitination[1]
300GHTLSWGKGQKDLLTubiquitination[2]
465QDWRKYYKVEPLDFGubiquitination[3, 4]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [4] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 
Functional Description
 Responsible for the degradation of GM2 gangliosides, and a variety of other molecules containing terminal N-acetyl hexosamines, in the brain and other tissues. 
Sequence Annotation
 ACT_SITE 355 355 Proton donor (By similarity).
 CARBOHYD 84 84 N-linked (GlcNAc...).
 CARBOHYD 142 142 N-linked (GlcNAc...).
 CARBOHYD 190 190 N-linked (GlcNAc...).
 CARBOHYD 323 323 N-linked (GlcNAc...).
 CARBOHYD 327 327 N-linked (GlcNAc...).
 DISULFID 91 137
 DISULFID 309 360
 DISULFID 534 551  
Keyword
 3D-structure; Complete proteome; Direct protein sequencing; Disease mutation; Disulfide bond; Gangliosidosis; Glycoprotein; Glycosidase; Hydrolase; Lysosome; Neurodegeneration; Polymorphism; Reference proteome; Signal; Zymogen. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 556 AA 
Protein Sequence
MELCGLGLPR PPMLLALLLA TLLAAMLALL TQVALVVQVA EAARAPSVSA KPGPALWPLP 60
LSVKMTPNLL HLAPENFYIS HSPNSTAGPS CTLLEEAFRR YHGYIFGFYK WHHEPAEFQA 120
KTQVQQLLVS ITLQSECDAF PNISSDESYT LLVKEPVAVL KANRVWGALR GLETFSQLVY 180
QDSYGTFTIN ESTIIDSPRF SHRGILIDTS RHYLPVKIIL KTLDAMAFNK FNVLHWHIVD 240
DQSFPYQSIT FPELSNKGSY SLSHVYTPND VRMVIEYARL RGIRVLPEFD TPGHTLSWGK 300
GQKDLLTPCY SRQNKLDSFG PINPTLNTTY SFLTTFFKEI SEVFPDQFIH LGGDEVEFKC 360
WESNPKIQDF MRQKGFGTDF KKLESFYIQK VLDIIATINK GSIVWQEVFD DKAKLAPGTI 420
VEVWKDSAYP EELSRVTASG FPVILSAPWY LDLISYGQDW RKYYKVEPLD FGGTQKQKQL 480
FIGGEACLWG EYVDATNLTP RLWPRASAVG ERLWSSKDVR DMDDAYDRLT RHRCRMVERG 540
IAAQPLYAGY CNHENM 556 
Gene Ontology
 GO:0001669; C:acrosomal vesicle; IEA:Compara.
 GO:0043202; C:lysosomal lumen; TAS:Reactome.
 GO:0016020; C:membrane; IEA:Compara.
 GO:0004563; F:beta-N-acetylhexosaminidase activity; TAS:ProtInc.
 GO:0046982; F:protein heterodimerization activity; IDA:MGI.
 GO:0042803; F:protein homodimerization activity; IDA:MGI.
 GO:0043615; P:astrocyte cell migration; IEA:Compara.
 GO:0005975; P:carbohydrate metabolic process; TAS:Reactome.
 GO:0008219; P:cell death; IEA:UniProtKB-KW.
 GO:0006874; P:cellular calcium ion homeostasis; IEA:Compara.
 GO:0044267; P:cellular protein metabolic process; IEA:Compara.
 GO:0030207; P:chondroitin sulfate catabolic process; TAS:Reactome.
 GO:0006689; P:ganglioside catabolic process; IEA:Compara.
 GO:0006687; P:glycosphingolipid metabolic process; TAS:Reactome.
 GO:0030214; P:hyaluronan catabolic process; TAS:Reactome.
 GO:0042340; P:keratan sulfate catabolic process; TAS:Reactome.
 GO:0019915; P:lipid storage; IEA:Compara.
 GO:0007626; P:locomotory behavior; IEA:Compara.
 GO:0007040; P:lysosome organization; IEA:Compara.
 GO:0008049; P:male courtship behavior; IEA:Compara.
 GO:0042552; P:myelination; IEA:Compara.
 GO:0050885; P:neuromuscular process controlling balance; IEA:Compara.
 GO:0009313; P:oligosaccharide catabolic process; IEA:Compara.
 GO:0048477; P:oogenesis; IEA:Compara.
 GO:0007341; P:penetration of zona pellucida; IEA:Compara.
 GO:0008654; P:phospholipid biosynthetic process; IEA:Compara.
 GO:0045944; P:positive regulation of transcription from RNA polymerase II promoter; IEA:Compara.
 GO:0008360; P:regulation of cell shape; IEA:Compara.
 GO:0007605; P:sensory perception of sound; IEA:Compara.
 GO:0001501; P:skeletal system development; IEA:Compara. 
Interpro
 IPR025705; Beta_hexosaminidase_sua/sub.
 IPR015883; Glyco_hydro_20_cat-core.
 IPR015882; Glyco_hydro_20b.
 IPR013781; Glyco_hydro_catalytic_dom.
 IPR017853; Glycoside_hydrolase_SF. 
Pfam
 PF00728; Glyco_hydro_20
 PF02838; Glyco_hydro_20b 
SMART
  
PROSITE
  
PRINTS
 PR00738; GLHYDRLASE20.