CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-009734
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 SUMO-conjugating enzyme UBC9 
Protein Synonyms/Alias
 SUMO-protein ligase; Ubiquitin carrier protein 9; mUBC9; Ubiquitin carrier protein I; Ubiquitin-conjugating enzyme E2 I; Ubiquitin-protein ligase I 
Gene Name
 Ube2i 
Gene Synonyms/Alias
 Ubc9; Ubce2i; Ubce9 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
18QERKAWRKDHPFGFVubiquitination[1]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 
Functional Description
 Accepts the ubiquitin-like proteins SUMO1, SUMO2 and SUMO3 from the UBLE1A-UBLE1B E1 complex and catalyzes their covalent attachment to other proteins with the help of an E3 ligase such as RANBP2 or CBX4. Can catalyze the formation of poly- SUMO chains. Essential for nuclear architecture, chromosome segregation and embryonic viability. Necessary for sumoylation of FOXL2 and KAT5 (By similarity). Sumoylates p53/TP53 at 'Lys-386'. 
Sequence Annotation
 REGION 13 18 Interaction with SUMO1 (By similarity).
 ACT_SITE 93 93 Glycyl thioester intermediate.
 MOD_RES 2 2 N-acetylserine (By similarity).
 MOD_RES 65 65 N6-acetyllysine (By similarity).
 MOD_RES 71 71 Phosphoserine; by CDK1 (By similarity).
 CROSSLNK 18 18 Glycyl lysine isopeptide (Lys-Gly)  
Keyword
 3D-structure; Acetylation; ATP-binding; Cell cycle; Cell division; Chromosome partition; Complete proteome; Cytoplasm; Developmental protein; Host-virus interaction; Isopeptide bond; Ligase; Mitosis; Nucleotide-binding; Nucleus; Phosphoprotein; Reference proteome; Ubl conjugation; Ubl conjugation pathway. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 158 AA 
Protein Sequence
MSGIALSRLA QERKAWRKDH PFGFVAVPTK NPDGTMNLMN WECAIPGKKG TPWEGGLFKL 60
RMLFKDDYPS SPPKCKFEPP LFHPNVYPSG TVCLSILEED KDWRPAITIK QILLGIQELL 120
NEPNIQDPAQ AEAYTIYCQN RVEYEKRVRA QAKKFAPS 158 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0030425; C:dendrite; IEA:Compara.
 GO:0001650; C:fibrillar center; IEA:Compara.
 GO:0016604; C:nuclear body; IDA:UniProtKB.
 GO:0016605; C:PML body; IEA:Compara.
 GO:0045202; C:synapse; IEA:Compara.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0019789; F:SUMO ligase activity; IEA:Compara.
 GO:0004842; F:ubiquitin-protein ligase activity; IEA:Compara.
 GO:0051301; P:cell division; IEA:UniProtKB-KW.
 GO:0006464; P:cellular protein modification process; TAS:ProtInc.
 GO:0007059; P:chromosome segregation; IEA:UniProtKB-KW.
 GO:0007067; P:mitosis; IEA:UniProtKB-KW.
 GO:0007275; P:multicellular organismal development; IEA:UniProtKB-KW.
 GO:0000122; P:negative regulation of transcription from RNA polymerase II promoter; IMP:BHF-UCL.
 GO:0033145; P:positive regulation of intracellular steroid hormone receptor signaling pathway; IEA:Compara.
 GO:0051091; P:positive regulation of sequence-specific DNA binding transcription factor activity; IEA:Compara.
 GO:0043161; P:proteasomal ubiquitin-dependent protein catabolic process; IEA:Compara.
 GO:0016925; P:protein sumoylation; IEA:UniProtKB-UniPathway.
 GO:0010469; P:regulation of receptor activity; IEA:Compara.
 GO:0019048; P:virus-host interaction; IEA:UniProtKB-KW. 
Interpro
 IPR027230; Ubc9.
 IPR000608; UBQ-conjugat_E2.
 IPR023313; UBQ-conjugating_AS.
 IPR016135; UBQ-conjugating_enzyme/RWD. 
Pfam
 PF00179; UQ_con 
SMART
  
PROSITE
 PS00183; UBIQUITIN_CONJUGAT_1
 PS50127; UBIQUITIN_CONJUGAT_2 
PRINTS