UniProt Accession

 SHS1_YEAST; Q07657; D6VRD0 

Genbank Protein ID

 Z74273; BK006938 

Genbank Nucleotide ID

 CAA98804.1; DAA11640.1 

Protein Name

 Seventh homolog of septin 1 

Protein Synonyms/Alias

 Septation protein 7 

Gene Name

 SHS1 

Gene Synonyms/Alias

 SEP7; YDL225W 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
57	PHKYQYGKSNASISS	acetylation	[1]
57	PHKYQYGKSNASISS	ubiquitination	[2]
82	KVVSFNSKNGIPSYV	acetylation	[1]
204	EVDVELMKSISKYTN	acetylation	[1]
321	GSHLQEFKDTTQNLL	ubiquitination	[2]
352	EIGPNSTKRQSNAPS	acetylation	[1]
352	EIGPNSTKRQSNAPS	ubiquitination	[2]
426	RQLGREIKQENENLI	sumoylation	[3, 4]
426	RQLGREIKQENENLI	ubiquitination	[2, 4]
437	ENLIRSIKTESSPKF	sumoylation	[3, 4]
437	ENLIRSIKTESSPKF	ubiquitination	[4]
443	IKTESSPKFLNSPDL	acetylation	[1]
478	RILARQQKLEELEAQ	acetylation	[1]
478	RILARQQKLEELEAQ	ubiquitination	[2]
488	ELEAQSAKELQKRIQ	acetylation	[1, 5]
488	ELEAQSAKELQKRIQ	ubiquitination	[2]
536	STRSQIKKNDTYTDL	acetylation	[1]
536	STRSQIKKNDTYTDL	ubiquitination	[2]

Reference

 [1] Regulation of septin dynamics by the Saccharomyces cerevisiae lysine acetyltransferase NuA4.
 Mitchell L, Lau A, Lambert JP, Zhou H, Fong Y, Couture JF, Figeys D, Baetz K.
 PLoS One. 2011;6(10):e25336. [PMID: 21984913]
 [2] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]
 [3] Sumoylation of topoisomerase I is involved in its partitioning between nucleoli and nucleoplasm and its clearing from nucleoli in response to camptothecin.
 Rallabhandi P, Hashimoto K, Mo YY, Beck WT, Moitra PK, D'Arpa P.
 J Biol Chem. 2002 Oct 18;277(42):40020-6. [PMID: 12149243]
 [4] Cytoplasmic sumoylation by PIAS-type Siz1-SUMO ligase.
 Takahashi Y, Iwase M, Strunnikov AV, Kikuchi Y.
 Cell Cycle. 2008 Jun 15;7(12):1738-44. [PMID: 18583943]
 [5] mChIP-KAT-MS, a method to map protein interactions and acetylation sites for lysine acetyltransferases.
 Mitchell L, Huard S, Cotrut M, Pourhanifeh-Lemeri R, Steunou AL, Hamza A, Lambert JP, Zhou H, Ning Z, Basu A, Côté J, Figeys DA, Baetz K.
 Proc Natl Acad Sci U S A. 2013 Apr 23;110(17):E1641-50. [PMID: 23572591] 

Functional Description

 Septins are GTPases involved in cytokinesis that assemble early in the cell cycle as a patch at the incipient bud site and form a ring approximate 15 min before bud emergence, which transforms into an hour-glass shaped collar of cortical filaments that spans both sides of the mother-bud neck. This collar persists until just before cytokinesis, when it splits into two rings that occupy opposite sides of the neck. The septins at the bud neck serve as a structural scaffold that recruits different components involved in diverse processes at specific stages during the cell cycle. Many proteins bind asymmetrically to the septin collar. The septin assembly is regulated by protein kinases GIN4 and/or CLA4. May act by recruiting MYO1 and HOF1, a protein involved in septation, to the site of cleavage. Septins are also involved in cell morphogenesis, bud site selection, chitin deposition, cell cycle regulation, cell compartmentalization and spore wall formation. 

Sequence Annotation

 NP_BIND 30 37 GTP (By similarity).
 NP_BIND 218 226 GTP (By similarity).
 BINDING 138 138 GTP; via amide nitrogen (By similarity).
 BINDING 288 288 GTP (By similarity).
 MOD_RES 400 400 Phosphotyrosine.
 MOD_RES 408 408 Phosphoserine.
 MOD_RES 416 416 Phosphoserine.
 MOD_RES 447 447 Phosphoserine.
 MOD_RES 460 460 Phosphoserine.
 MOD_RES 519 519 Phosphoserine.
 MOD_RES 520 520 Phosphoserine.
 MOD_RES 522 522 Phosphoserine.
 MOD_RES 525 525 Phosphoserine.
 MOD_RES 539 539 Phosphothreonine.
 MOD_RES 545 545 Phosphoserine.
 MOD_RES 548 548 Phosphoserine.
 CROSSLNK 426 426 Glycyl lysine isopeptide (Lys-Gly)
 CROSSLNK 437 437 Glycyl lysine isopeptide (Lys-Gly)  

Keyword

 Cell cycle; Cell division; Coiled coil; Complete proteome; GTP-binding; Isopeptide bond; Membrane; Nucleotide-binding; Phosphoprotein; Reference proteome; Ubl conjugation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 551 AA 

Protein Sequence

Gene Ontology

 GO:0000144; C:cellular bud neck septin ring; IDA:SGD.
 GO:0043332; C:mating projection tip; IDA:SGD.
 GO:0016020; C:membrane; IEA:UniProtKB-SubCell.
 GO:0031105; C:septin complex; IEA:InterPro.
 GO:0005525; F:GTP binding; IDA:SGD.
 GO:0000917; P:barrier septum assembly; IMP:SGD.
 GO:0032186; P:cellular bud neck septin ring organization; IMP:SGD.
 GO:0000915; P:cytokinesis, actomyosin contractile ring assembly; IMP:SGD.
 GO:0010458; P:exit from mitosis; IMP:SGD.
 GO:0000921; P:septin ring assembly; IGI:SGD. 

Interpro

 IPR000038; Cell_div_GTP-bd.
 IPR027417; P-loop_NTPase.
 IPR016491; Septin. 

Pfam

 PF00735; Septin 

SMART

  

PROSITE

  

PRINTS