CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-019297
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Centromere protein N 
Protein Synonyms/Alias
 CENP-N; Interphase centromere complex protein 32 
Gene Name
 CENPN 
Gene Synonyms/Alias
 C16orf60; ICEN32; BM-309 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
10ETVAEFIKRTILKIPubiquitination[1]
15FIKRTILKIPMNELTubiquitination[1, 2]
90WEVFQMSKGPGEDVDubiquitination[2]
102DVDLFDMKQFKNSFKubiquitination[1, 2]
117KILQRALKNVTVSFRubiquitination[1, 2]
202SRYLDSLKAIVFKQYubiquitination[1, 2, 3]
207SLKAIVFKQYNQTFEubiquitination[4, 5]
245IHENIVEKERVQRITubiquitination[1, 2, 6]
270QLEFAQYKLETKFKSubiquitination[2, 3]
274AQYKLETKFKSGLNGubiquitination[1]
276YKLETKFKSGLNGSIubiquitination[1, 4, 5]
296EPLRCLIKFSSPHLLubiquitination[1]
329LLTCIPNKRMNYFKIubiquitination[1, 6]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [4] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [5] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [6] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302
Functional Description
 Component of the CENPA-NAC (nucleosome-associated) complex, a complex that plays a central role in assembly of kinetochore proteins, mitotic progression and chromosome segregation. The CENPA-NAC complex recruits the CENPA-CAD (nucleosome distal) complex and may be involved in incorporation of newly synthesized CENPA into centromeres. CENPN is the first protein to bind specifically to CENPA nucleosomes and the direct binding of CENPA nucleosomes by CENPN is required for centromere assembly. Required for chromosome congression and efficiently align the chromosomes on a metaphase plate. 
Sequence Annotation
 MOD_RES 226 226 Phosphoserine.
 MOD_RES 282 282 Phosphoserine.  
Keyword
 Alternative splicing; Centromere; Chromosome; Complete proteome; Kinetochore; Nucleus; Phosphoprotein; Polymorphism; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 339 AA 
Protein Sequence
MDETVAEFIK RTILKIPMNE LTTILKAWDF LSENQLQTVN FRQRKESVVQ HLIHLCEEKR 60
ASISDAALLD IIYMQFHQHQ KVWEVFQMSK GPGEDVDLFD MKQFKNSFKK ILQRALKNVT 120
VSFRETEENA VWIRIAWGTQ YTKPNQYKPT YVVYYSQTPY AFTSSSMLRR NTPLLGQALT 180
IASKHHQIVK MDLRSRYLDS LKAIVFKQYN QTFETHNSTT PLQERSLGLD INMDSRIIHE 240
NIVEKERVQR ITQETFGDYP QPQLEFAQYK LETKFKSGLN GSILAEREEP LRCLIKFSSP 300
HLLEALKSLA PAGIADAPLS PLLTCIPNKR MNYFKIRDK 339 
Gene Ontology
 GO:0000777; C:condensed chromosome kinetochore; IEA:UniProtKB-SubCell.
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0005654; C:nucleoplasm; TAS:Reactome.
 GO:0034080; P:CENP-A containing nucleosome assembly at centromere; TAS:Reactome.
 GO:0000278; P:mitotic cell cycle; TAS:Reactome. 
Interpro
 IPR007902; CHL4. 
Pfam
 PF05238; CENP-N 
SMART
  
PROSITE
  
PRINTS