UniProt Accession

 GSTA_ECOLI; P0A9D2; P39100 

Genbank Protein ID

 D38497; U00096; AP009048 

Genbank Nucleotide ID

 BAA07509.1; AAC74707.1; BAA15396.1 

Protein Name

 Glutathione S-transferase GstA 

Protein Synonyms/Alias

 GST B1-1 

Gene Name

 gstA 

Gene Synonyms/Alias

 gst; b1635; JW1627 

Created Date

 July 27, 2013 

Organism

 Escherichia coli (strain K12) 

NCBI Taxa ID

 83333 

Lysine Modification

Position	Peptide	Type	References
2	******MKLFYKPGA	acetylation	[1]
6	**MKLFYKPGACSLA	acetylation	[1, 2]
23	ITLRESGKDFTLVSV	acetylation	[1]
34	LVSVDLMKKRLENGD	acetylation	[1]
122	PDTPEEYKPTVRAQL	acetylation	[1]
131	TVRAQLEKKLQYVNE	acetylation	[1]
132	VRAQLEKKLQYVNEA	acetylation	[1]
141	QYVNEALKDEHWICG	acetylation	[1]

Reference

 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618]
 [2] Comprehensive profiling of protein lysine acetylation in Escherichia coli.
 Zhang K, Zheng S, Yang JS, Chen Y, Cheng Z.
 J Proteome Res. 2013 Feb 1;12(2):844-51. [PMID: 23294111] 

Functional Description

 Catalyzes the conjugation of reduced glutathione (GSH) to a wide number of exogenous and endogenous hydrophobic electrophiles. Shows activity toward 1-chloro-2,4-dinitrobenzene (CDNB) and ethacrynic acid. Also possesses thiol:disulfide oxidoreductase activity, using GSH to reduce bis-(2-hydroxyethyl) disulfide (HEDS). Has a low level of glutathione-dependent peroxidase activity toward cumene hydroperoxide. Is important for defense against oxidative stress, probably via its peroxidase activity. 

Sequence Annotation

 DOMAIN 1 81 GST N-terminal.
 DOMAIN 87 201 GST C-terminal.
 REGION 65 66 Glutathione binding.
 REGION 103 106 Glutathione binding.
 BINDING 10 10 Glutathione.
 BINDING 35 35 Glutathione.
 BINDING 52 52 Glutathione; via amide nitrogen and
 BINDING 99 99 Glutathione.  

Keyword

 3D-structure; Complete proteome; Cytoplasm; Direct protein sequencing; Reference proteome; Transferase. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 201 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0004364; F:glutathione transferase activity; IDA:EcoCyc.
 GO:0042178; P:xenobiotic catabolic process; TAS:EcoCyc. 

Interpro

 IPR010987; Glutathione-S-Trfase_C-like.
 IPR004045; Glutathione_S-Trfase_N.
 IPR017933; Glutathione_S_Trfase/Cl_chnl_C.
 IPR004046; GST_C.
 IPR012336; Thioredoxin-like_fold. 

Pfam

 PF00043; GST_C 

SMART

  

PROSITE

 PS50405; GST_CTER
 PS50404; GST_NTER 

PRINTS