UniProt Accession

 RAB35_HUMAN; Q15286; B2R6E0; B4E390 

Genbank Protein ID

 X79781; AF498960; BT020024; CR536486; CR541683; AK304620; AK312538; AC004812; CH471054; CH471054; BC015931 

Genbank Nucleotide ID

 CAA56177.1; AAM21108.1; AAV38827.1; CAG38725.1; CAG46484.1; BAG65402.1; BAG35437.1; AAC83182.1; EAW98163.1; EAW98164.1; AAH15931.1 

Protein Name

 Ras-related protein Rab-35 

Protein Synonyms/Alias

 GTP-binding protein RAY; Ras-related protein Rab-1C 

Gene Name

 RAB35 

Gene Synonyms/Alias

 RAB1C; RAY 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
21	IGDSGVGKSSLLLRF	ubiquitination	[1]
58	EINGEKVKLQIWDTA	ubiquitination	[2]
121	CRILVGNKNDDPERK	ubiquitination	[3]
128	KNDDPERKVVETEDA	ubiquitination	[3]
178	AKKDNLAKQQQQQQN	ubiquitination	[1, 2, 3, 4, 5, 6]
189	QQQNDVVKLTKNSKR	ubiquitination	[1, 2, 3, 5, 6]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [4] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
 Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
 J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572]
 [5] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [6] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661] 

Functional Description

 In the process of endocytosis, essential rate-limiting regulator of a fast recycling pathway back to the plasma membrane. During cytokinesis, required for the postfurrowing terminal steps, namely for intercellular bridge stability and abscission, possibly by controlling phosphatidylinositol 4,5-bis phosphate (PIP2) and SEPT2 localization at the intercellular bridge. 

Sequence Annotation

 NP_BIND 15 22 GTP (By similarity).
 NP_BIND 63 67 GTP (By similarity).
 NP_BIND 120 123 GTP (By similarity).
 MOTIF 37 45 Effector region (By similarity).
 MOD_RES 75 75 O-(2-cholinephosphoryl)serine; by
 MOD_RES 77 77 O-AMP-tyrosine; by Legionella DrrA
 LIPID 200 200 S-geranylgeranyl cysteine (By
 LIPID 201 201 S-geranylgeranyl cysteine (By  

Keyword

 3D-structure; Alternative splicing; Cell membrane; Coated pit; Complete proteome; Cytoplasmic vesicle; Endosome; GTP-binding; Lipoprotein; Membrane; Nucleotide-binding; Phosphoprotein; Prenylation; Protein transport; Reference proteome; Transport. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 201 AA 

Protein Sequence

Gene Ontology

 GO:0031253; C:cell projection membrane; IDA:UniProtKB.
 GO:0045334; C:clathrin-coated endocytic vesicle; IDA:UniProtKB.
 GO:0005905; C:coated pit; IDA:UniProtKB.
 GO:0005768; C:endosome; IEA:UniProtKB-SubCell.
 GO:0045171; C:intercellular bridge; IDA:UniProtKB.
 GO:0042470; C:melanosome; IEA:UniProtKB-SubCell.
 GO:0005739; C:mitochondrion; IEA:Compara.
 GO:0019003; F:GDP binding; IDA:UniProtKB.
 GO:0005525; F:GTP binding; IDA:UniProtKB.
 GO:0003924; F:GTPase activity; TAS:ProtInc.
 GO:0005546; F:phosphatidylinositol-4,5-bisphosphate binding; IDA:UniProtKB.
 GO:0000910; P:cytokinesis; IMP:UniProtKB.
 GO:0016197; P:endosomal transport; IMP:UniProtKB.
 GO:0008104; P:protein localization; IMP:UniProtKB.
 GO:0015031; P:protein transport; IEA:UniProtKB-KW.
 GO:0007264; P:small GTPase mediated signal transduction; IEA:InterPro. 

Interpro

 IPR027417; P-loop_NTPase.
 IPR005225; Small_GTP-bd_dom.
 IPR001806; Small_GTPase.
 IPR003579; Small_GTPase_Rab_type. 

Pfam

 PF00071; Ras 

SMART

 SM00175; RAB 

PROSITE

 PS51419; RAB 

PRINTS

 PR00449; RASTRNSFRMNG.