UniProt Accession

 OPI1_YEAST; P21957; D3DKP9 

Genbank Protein ID

 M57383; U11582; AY558103; BK006934 

Genbank Nucleotide ID

 AAA34828.1; AAB65073.1; AAS56429.1; DAA06665.1 

Protein Name

 Transcriptional repressor OPI1 

Protein Synonyms/Alias

 Negative regulator of phospholipid biosynthesis; Overproducer of inositol protein 1 

Gene Name

 OPI1 

Gene Synonyms/Alias

 YHL020C 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
42	EDVSQADKMPASESS	acetylation	[1]
151	HLLKLANKQLSDKIS	ubiquitination	[2]
227	EVVGTVKKVYSLISK	ubiquitination	[2]
234	KVYSLISKFTANSLP	ubiquitination	[2]
314	QQQQKRNKDGDDSAS	ubiquitination	[2]
394	QKDGNYVKPSQDNVD	ubiquitination	[2]

Reference

 [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919]
 [2] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301] 

Functional Description

 Negative regulator of the transcriptional complex INO2- INO4 in response to phospholipid precursor availability. When precursors become limiting, OPI1 is retained at the endoplasmic reticulum (ER) and INO2-INO4 activates INO1 and other genes required for phospholipid biosynthesis, whereas abundant precursor availability results in targeting of OPI1 to the nucleus to repress transcription of these genes. Binds directly to phosphatidic acid, which is required for ER targeting and may act as sensing mechanism for precursor availability, as phosphatidic acid becomes rapidly depleted upon phospholipid biosynthesis. 

Sequence Annotation

 REGION 109 138 Basic motif.
 REGION 139 160 Leucine-zipper.
 MOTIF 200 206 FFAT.
 MOD_RES 10 10 Phosphoserine.  

Keyword

 Complete proteome; DNA-binding; Endoplasmic reticulum; Lipid biosynthesis; Lipid metabolism; Nucleus; Phospholipid biosynthesis; Phospholipid metabolism; Phosphoprotein; Reference proteome; Repressor; Transcription; Transcription regulation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 404 AA 

Protein Sequence

Gene Ontology

 GO:0005783; C:endoplasmic reticulum; IDA:SGD.
 GO:0031965; C:nuclear membrane; IDA:SGD.
 GO:0005654; C:nucleoplasm; IDA:SGD.
 GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
 GO:0003714; F:transcription corepressor activity; IPI:SGD.
 GO:0030968; P:endoplasmic reticulum unfolded protein response; IMP:SGD.
 GO:0000122; P:negative regulation of transcription from RNA polymerase II promoter; IMP:SGD.
 GO:0008654; P:phospholipid biosynthetic process; IMP:SGD.
 GO:0045944; P:positive regulation of transcription from RNA polymerase II promoter; IMP:SGD.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 

Interpro

 IPR013927; TF_Opi1. 

Pfam

 PF08618; Opi1 

SMART

  

PROSITE

  

PRINTS