CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-031093
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Makorin, ring finger protein, 2, isoform CRA_b 
Protein Synonyms/Alias
 Probable E3 ubiquitin-protein ligase makorin-2; cDNA FLJ52346, highly similar to Makorin-2 
Gene Name
 MKRN2 
Gene Synonyms/Alias
 hCG_28319 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
4****MSTKQITCRYDubiquitination[1, 2, 3]
100PQPSPEMKPHSYLDAubiquitination[2]
171EQRKAHEKICMLTFEubiquitination[2]
235IRQWRCAKQFENPIIubiquitination[2]
243QFENPIIKSCPECRVubiquitination[3]
267YWVEDQNKKNELIEAubiquitination[1]
268WVEDQNKKNELIEAFubiquitination[2, 3]
276NELIEAFKQGMGKKAubiquitination[2]
285GMGKKACKYFEQGKGubiquitination[2]
291CKYFEQGKGTCPFGSubiquitination[1, 2, 3]
299GTCPFGSKCLYRHAYubiquitination[2, 3]
316GRLAEPEKPRKQLSSubiquitination[2, 3]
319AEPEKPRKQLSSQGTubiquitination[2]
Reference
 [1] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302] 
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Metal-binding; Reference proteome; Zinc; Zinc-finger. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 373 AA 
Protein Sequence
MSTKQITCRY DHTRPSAAAG GAVGTMAHSV PSPAFHSPHP PSEVTASIVK TNSHEPGKRE 60
KRTLVLRDRN LSGMAERKTQ PSMVSNPGSC SDPQPSPEMK PHSYLDAIRS GLDDVEASSS 120
YSNEQQLCPY AAAGECRFGD ACVYLHGEVC EICRLQVLHP FDPEQRKAHE KICMLTFEHE 180
MEKAFAFQAS QDKVCSICME VILEKASASE RRFGILSNCN HTYCLSCIRQ WRCAKQFENP 240
IIKSCPECRV ISEFVIPSVY WVEDQNKKNE LIEAFKQGMG KKACKYFEQG KGTCPFGSKC 300
LYRHAYPDGR LAEPEKPRKQ LSSQGTVRFF NSVRLWDFIE NRESRHVPNN EDVDMTELGD 360
LFMHLSGVES SEP 373 
Gene Ontology
 GO:0003676; F:nucleic acid binding; IEA:InterPro.
 GO:0008270; F:zinc ion binding; IEA:InterPro. 
Interpro
 IPR026290; Makorin-related.
 IPR026293; Makorin_2.
 IPR000571; Znf_CCCH.
 IPR001841; Znf_RING.
 IPR013083; Znf_RING/FYVE/PHD.
 IPR017907; Znf_RING_CS. 
Pfam
 PF00642; zf-CCCH
 PF13639; zf-RING_2 
SMART
 SM00184; RING
 SM00356; ZnF_C3H1 
PROSITE
 PS50103; ZF_C3H1
 PS00518; ZF_RING_1
 PS50089; ZF_RING_2 
PRINTS