UniProt Accession

 MAOX_MOUSE; P06801; Q9DBF9 

Genbank Protein ID

 J02652; M26756; AK004980; AK077968 

Genbank Nucleotide ID

 AAA39727.1; AAA39489.1; BAB23716.1; BAC37086.1 

Protein Name

 NADP-dependent malic enzyme 

Protein Synonyms/Alias

 NADP-ME; Malic enzyme 1 

Gene Name

 Me1 

Gene Synonyms/Alias

 Mod-1; Mod1 

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
26	TRDPHLNKDLAFTLE	ubiquitination	[1]
60	LQVLRIIKNFERLNS	ubiquitination	[1]
324	MEKEGLSKENARKKI	acetylation	[2]
330	SKENARKKIWLVDSK	acetylation	[3]
330	SKENARKKIWLVDSK	ubiquitination	[1]
337	KIWLVDSKGLIVKGR	acetylation	[4]
337	KIWLVDSKGLIVKGR	ubiquitination	[1]
351	RASLTEEKEVFAHEH	acetylation	[2]
362	AHEHEEMKNLEAIVQ	acetylation	[5]
362	AHEHEEMKNLEAIVQ	ubiquitination	[1]
422	CSAEQCYKVTKGRAI	acetylation	[2]
422	CSAEQCYKVTKGRAI	ubiquitination	[1]
476	GLRHIDDKVFLTTAE	ubiquitination	[1]
492	ISQQVSDKHLQEGRL	ubiquitination	[1]
516	VSLKIAVKIVQDAYK	ubiquitination	[1]
523	KIVQDAYKEKMATVY	acetylation	[2, 5]
523	KIVQDAYKEKMATVY	ubiquitination	[1]
525	VQDAYKEKMATVYPE	ubiquitination	[1]

Reference

 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [2] Calorie restriction and SIRT3 trigger global reprogramming of the mitochondrial protein acetylome.
 Hebert AS, Dittenhafer-Reed KE, Yu W, Bailey DJ, Selen ES, Boersma MD, Carson JJ, Tonelli M, Balloon AJ, Higbee AJ, Westphall MS, Pagliarini DJ, Prolla TA, Assadi-Porter F, Roy S, Denu JM, Coon JJ.
 Mol Cell. 2013 Jan 10;49(1):186-99. [PMID: 23201123]
 [3] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
 [4] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [5] Quantitative assessment of the impact of the gut microbiota on lysine epsilon-acetylation of host proteins using gnotobiotic mice.
 Simon GM, Cheng J, Gordon JI.
 Proc Natl Acad Sci U S A. 2012 Jul 10;109(28):11133-8. [PMID: 22733758] 

Functional Description

  

Sequence Annotation

 NP_BIND 301 318 NADP (By similarity).
 ACT_SITE 102 102 Proton donor (By similarity).
 ACT_SITE 173 173 Proton acceptor (By similarity).
 METAL 245 245 Divalent metal cation (By similarity).
 METAL 246 246 Divalent metal cation (By similarity).
 METAL 269 269 Divalent metal cation (By similarity).
 BINDING 155 155 NADP (By similarity).
 BINDING 269 269 NADP (By similarity).
 MOD_RES 1 1 N-acetylmethionine (By similarity).  

Keyword

 Acetylation; Complete proteome; Cytoplasm; Direct protein sequencing; Metal-binding; NADP; Oxidoreductase; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 572 AA 

Protein Sequence

Gene Ontology

 GO:0005829; C:cytosol; ISS:UniProtKB.
 GO:0005739; C:mitochondrion; IDA:MGI.
 GO:0004473; F:malate dehydrogenase (oxaloacetate-decarboxylating) (NADP+) activity; ISS:UniProtKB.
 GO:0016619; F:malate dehydrogenase (oxaloacetate-decarboxylating) activity; IEA:InterPro.
 GO:0030145; F:manganese ion binding; ISS:UniProtKB.
 GO:0051287; F:NAD binding; IEA:InterPro.
 GO:0006108; P:malate metabolic process; ISS:UniProtKB.
 GO:0051262; P:protein tetramerization; IDA:MGI.
 GO:0009725; P:response to hormone stimulus; ISS:UniProtKB. 

Interpro

 IPR015884; Malic_enzyme_CS.
 IPR012301; Malic_N.
 IPR012302; Malic_NAD-bd.
 IPR001891; Malic_OxRdtase.
 IPR016040; NAD(P)-bd_dom. 

Pfam

 PF00390; malic
 PF03949; Malic_M 

SMART

 SM00919; Malic_M 

PROSITE

 PS00331; MALIC_ENZYMES 

PRINTS

 PR00072; MALOXRDTASE.