UniProt Accession

 AATC_RAT; P13221; Q64570; Q6P721 

Genbank Protein ID

 J04171; D00252; BC061877; J05263 

Genbank Nucleotide ID

 AAA40769.1; BAA00183.1; AAH61877.1; AAA40842.1 

Protein Name

 Aspartate aminotransferase, cytoplasmic 

Protein Synonyms/Alias

 cAspAT; Cysteine aminotransferase, cytoplasmic; Cysteine transaminase, cytoplasmic; cCAT; Glutamate oxaloacetate transaminase 1; Transaminase A 

Gene Name

 Got1 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Rattus norvegicus (Rat) 

NCBI Taxa ID

 10116 

Lysine Modification

Position	Peptide	Type	References
56	WVLPVVRKVEQKIAN	acetylation	[1]
60	VVRKVEQKIANDHSL	acetylation	[1]
154	VFSAAGFKDIRSYRY	acetylation	[1]
166	YRYWDAEKRGLDLQG	acetylation	[1]
214	KQIAAVMKRRFLFPF	acetylation	[1]
276	GNLTVVGKEHDSVLR	acetylation	[1]
290	RVLSQMEKIVRITWS	acetylation	[1]
290	RVLSQMEKIVRITWS	ubiquitination	[2]
318	LSNPELFKEWKGNVK	acetylation	[1]
318	LSNPELFKEWKGNVK	ubiquitination	[2]
321	PELFKEWKGNVKTMA	acetylation	[1]
325	KEWKGNVKTMADRIL	acetylation	[1]
378	VEYLVNEKHIYLMPS	acetylation	[1]
411	SINEAVTKFQ*****	acetylation	[1]

Reference

 [1] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns.
 Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV.
 Cell Rep. 2012 Aug 30;2(2):419-31. [PMID: 22902405]
 [2] Synaptic protein ubiquitination in rat brain revealed by antibody-based ubiquitome analysis.
 Na CH, Jones DR, Yang Y, Wang X, Xu Y, Peng J.
 J Proteome Res. 2012 Sep 7;11(9):4722-32. [PMID: 22871113] 

Functional Description

 Biosynthesis of L-glutamate from L-aspartate or L- cysteine. Important regulator of levels of glutamate, the major excitatory neurotransmitter of the vertebrate central nervous system. Acts as a scavenger of glutamate in brain neuroprotection. The aspartate aminotransferase activity is involved in hepatic glucose synthesis during development and in adipocyte glyceroneogenesis. Using L-cysteine as substrate, regulates levels of mercaptopyruvate, an important source of hydrogen sulfide. Mercaptopyruvate is converted into H(2)S via the action of 3- mercaptopyruvate sulfurtransferase (3MST). Hydrogen sulfide is an important synaptic modulator and neuroprotectant in the brain. 

Sequence Annotation

 BINDING 39 39 Aspartate; via amide nitrogen (By
 BINDING 141 141 Aspartate (By similarity).
 BINDING 195 195 Aspartate (By similarity).
 BINDING 387 387 Aspartate (By similarity).
 MOD_RES 66 66 Phosphoserine (By similarity).
 MOD_RES 71 71 Phosphotyrosine (By similarity).
 MOD_RES 259 259 N6-(pyridoxal phosphate)lysine.  

Keyword

 Amino-acid biosynthesis; Aminotransferase; Complete proteome; Cytoplasm; Direct protein sequencing; Phosphoprotein; Pyridoxal phosphate; Reference proteome; Transferase. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 413 AA 

Protein Sequence

Gene Ontology

 GO:0043679; C:axon terminus; IDA:RGD.
 GO:0005829; C:cytosol; TAS:RGD.
 GO:0005764; C:lysosome; IDA:RGD.
 GO:0031406; F:carboxylic acid binding; IDA:RGD.
 GO:0004069; F:L-aspartate:2-oxoglutarate aminotransferase activity; ISS:UniProtKB.
 GO:0047801; F:L-cysteine:2-oxoglutarate aminotransferase activity; IDA:UniProtKB.
 GO:0080130; F:L-phenylalanine:2-oxoglutarate aminotransferase activity; IEA:EC.
 GO:0004609; F:phosphatidylserine decarboxylase activity; IEA:Compara.
 GO:0030170; F:pyridoxal phosphate binding; IEA:InterPro.
 GO:0006103; P:2-oxoglutarate metabolic process; ISS:UniProtKB.
 GO:0006532; P:aspartate biosynthetic process; IEA:Compara.
 GO:0006533; P:aspartate catabolic process; IEA:Compara.
 GO:0006531; P:aspartate metabolic process; ISS:UniProtKB.
 GO:0032869; P:cellular response to insulin stimulus; IEA:Compara.
 GO:0055089; P:fatty acid homeostasis; IEA:Compara.
 GO:0019551; P:glutamate catabolic process to 2-oxoglutarate; IEA:Compara.
 GO:0019550; P:glutamate catabolic process to aspartate; IEA:Compara.
 GO:0006536; P:glutamate metabolic process; ISS:UniProtKB.
 GO:0006114; P:glycerol biosynthetic process; IDA:UniProtKB.
 GO:0006107; P:oxaloacetate metabolic process; IEA:Compara.
 GO:0051384; P:response to glucocorticoid stimulus; IEA:Compara. 

Interpro

 IPR004839; Aminotransferase_I/II.
 IPR000796; Asp_trans.
 IPR004838; NHTrfase_class1_PyrdxlP-BS.
 IPR015424; PyrdxlP-dep_Trfase.
 IPR015421; PyrdxlP-dep_Trfase_major_sub1. 

Pfam

 PF00155; Aminotran_1_2 

SMART

  

PROSITE

 PS00105; AA_TRANSFER_CLASS_1 

PRINTS

 PR00799; TRANSAMINASE.