CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-022109
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Ras association domain-containing protein 1 
Protein Synonyms/Alias
  
Gene Name
 RASSF1 
Gene Synonyms/Alias
 RDA32 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
236VIEALLRKFLVVDDPubiquitination[1]
Reference
 [1] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789
Functional Description
 Potential tumor suppressor. Required for death receptor- dependent apoptosis. Mediates activation of STK3/MST2 and STK4/MST1 during Fas-induced apoptosis by preventing their dephosphorylation. When associated with MOAP1, promotes BAX conformational change and translocation to mitochondrial membranes in response to TNF and TNFSF10 stimulation. Isoform A interacts with CDC20, an activator of the anaphase-promoting complex, APC, resulting in the inhibition of APC activity and mitotic progression. Inhibits proliferation by negatively regulating cell cycle progression at the level of G1/S-phase transition by regulating accumulation of cyclin D1 protein. Isoform C has been shown not to perform these roles, no function has been identified for this isoform. Isoform A disrupts interactions among MDM2, DAXX and USP7, thus contributing to the efficient activation of TP53 by promoting MDM2 self-ubiquitination in cell-cycle checkpoint control in response to DNA damage. 
Sequence Annotation
 DOMAIN 198 292 Ras-associating.
 DOMAIN 294 341 SARAH.
 ZN_FING 51 105 Phorbol-ester/DAG-type.
 REGION 2 119 Mediates interaction with E4F1.
 REGION 315 318 MOAP1-binding.
 MOD_RES 2 2 N-acetylserine.
 MOD_RES 2 2 Phosphoserine.  
Keyword
 3D-structure; Acetylation; Alternative promoter usage; Alternative splicing; Cell cycle; Complete proteome; Cytoplasm; Cytoskeleton; Metal-binding; Microtubule; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Tumor suppressor; Zinc; Zinc-finger. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 344 AA 
Protein Sequence
MSGEPELIEL RELAPAGRAG KGRTRLERAN ALRIARGTAC NPTRQLVPGR GHRFQPAGPA 60
THTWCDLCGD FIWGVVRKGL QCAHCKFTCH YRCRALVCLD CCGPRDLGWE PAVERDTNVP 120
ILQDEPVEWE TPDLSQAEIE QKIKEYNAQI NSNLFMSLNK DGSYTGFIKV QLKLVRPVSV 180
PSSKKPPSLQ DARRGPGRGT SVRRRTSFYL PKDAVKHLHV LSRTRAREVI EALLRKFLVV 240
DDPRKFALFE RAERHGQVYL RKLLDDEQPL RLRLLAGPSD KALSFVLKEN DSGEVNWDAF 300
SMPELHNFLR ILQREEEEHL RQILQKYSYC RQKIQEALHA CPLG 344 
Gene Ontology
 GO:0005737; C:cytoplasm; IDA:HPA.
 GO:0005874; C:microtubule; IEA:UniProtKB-KW.
 GO:0015630; C:microtubule cytoskeleton; IDA:UniProtKB.
 GO:0005815; C:microtubule organizing center; IEA:UniProtKB-SubCell.
 GO:0005634; C:nucleus; IDA:UniProtKB.
 GO:0005886; C:plasma membrane; IDA:HPA.
 GO:0000922; C:spindle pole; IEA:UniProtKB-SubCell.
 GO:0008270; F:zinc ion binding; TAS:UniProtKB.
 GO:0007050; P:cell cycle arrest; IMP:UniProtKB.
 GO:0071157; P:negative regulation of cell cycle arrest; IDA:UniProtKB.
 GO:0031398; P:positive regulation of protein ubiquitination; IDA:UniProtKB.
 GO:0050821; P:protein stabilization; IDA:UniProtKB.
 GO:0007265; P:Ras protein signal transduction; IDA:UniProtKB.
 GO:0006974; P:response to DNA damage stimulus; IMP:UniProtKB. 
Interpro
 IPR002219; Prot_Kinase_C-like_PE/DAG-bd.
 IPR000159; Ras-assoc.
 IPR011524; SARAH_dom. 
Pfam
 PF00130; C1_1
 PF00788; RA 
SMART
 SM00109; C1
 SM00314; RA 
PROSITE
 PS50200; RA
 PS50951; SARAH
 PS00479; ZF_DAG_PE_1
 PS50081; ZF_DAG_PE_2 
PRINTS