UniProt Accession

 IF4A3_RAT; Q3B8Q2 

Genbank Protein ID

 BC105875; CH473948 

Genbank Nucleotide ID

 AAI05876.1; EDM06783.1 

Protein Name

 Eukaryotic initiation factor 4A-III 

Protein Synonyms/Alias

 eIF-4A-III; eIF4A-III; ATP-dependent RNA helicase DDX48; ATP-dependent RNA helicase eIF4A-3; DEAD box protein 48; Eukaryotic translation initiation factor 4A isoform 3; Eukaryotic initiation factor 4A-III, N-terminally processed 

Gene Name

 Eif4a3 

Gene Synonyms/Alias

 Ddx48 

Created Date

 July 27, 2013 

Organism

 Rattus norvegicus (Rat) 

NCBI Taxa ID

 10116 

Lysine Modification

Position	Peptide	Type	References
60	IYAYGFEKPSAIQQR	acetylation	[1]
60	IYAYGFEKPSAIQQR	ubiquitination	[2]
70	AIQQRAIKQIIKGRD	acetylation	[1]
321	KERESIMKEFRSGAS	acetylation	[1]

Reference

 [1] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns.
 Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV.
 Cell Rep. 2012 Aug 30;2(2):419-31. [PMID: 22902405]
 [2] Synaptic protein ubiquitination in rat brain revealed by antibody-based ubiquitome analysis.
 Na CH, Jones DR, Yang Y, Wang X, Xu Y, Peng J.
 J Proteome Res. 2012 Sep 7;11(9):4722-32. [PMID: 22871113] 

Functional Description

 ATP-dependent RNA helicase. Component of a splicing- dependent multiprotein exon junction complex (EJC) deposited at splice junction on mRNAs. The EJC is a dynamic structure consisting of a few core proteins and several more peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. Core components of the EJC, that remains bound to spliced mRNAs throughout all stages of mRNA metabolism, functions to mark the position of the exon-exon junction in the mature mRNA and thereby influences downstream processes of gene expression including mRNA splicing, nuclear mRNA export, subcellular mRNA localization, translation efficiency and nonsense-mediated mRNA decay (NMD). Constitutes at least part of the platform anchoring other EJC proteins to spliced mRNAs. Its RNA-dependent ATPase and RNA-helicase activities are induced by CASC3, but abolished in presence of the MAGOH/RBM8A heterodimer, thereby trapping the ATP- bound EJC core onto spliced mRNA in a stable conformation. The inhibition of ATPase activity by the MAGOH/RBM8A heterodimer increases the RNA-binding affinity of the EJC. Involved in translational enhancement of spliced mRNAs after formation of the 80S ribosome complex. Binds spliced mRNA in sequence-independent manner, 20-24 nucleotides upstream of mRNA exon-exon junctions. Shows higher affinity for single-stranded RNA in an ATP-bound core EJC complex than after the ATP is hydrolyzed (By similarity). 

Sequence Annotation

 DOMAIN 69 239 Helicase ATP-binding.
 DOMAIN 250 411 Helicase C-terminal.
 NP_BIND 85 90 ATP (By similarity).
 NP_BIND 367 371 ATP (By similarity).
 MOTIF 38 66 Q motif.
 MOTIF 187 190 DEAD box.
 BINDING 60 60 ATP; via carbonyl oxygen (By similarity).
 BINDING 65 65 ATP (By similarity).
 BINDING 342 342 ATP (By similarity).
 MOD_RES 1 1 N-acetylmethionine (By similarity).
 MOD_RES 2 2 N-acetylalanine; in Eukaryotic initiation
 MOD_RES 12 12 Phosphoserine (By similarity).
 MOD_RES 163 163 Phosphothreonine (By similarity).
 MOD_RES 296 296 N6-acetyllysine (By similarity).
 MOD_RES 321 321 N6-acetyllysine (By similarity).  

Keyword

 Acetylation; ATP-binding; Complete proteome; Cytoplasm; Helicase; Hydrolase; mRNA processing; mRNA splicing; mRNA transport; Nonsense-mediated mRNA decay; Nucleotide-binding; Nucleus; Phosphoprotein; Reference proteome; RNA-binding; rRNA processing; Spliceosome; Translation regulation; Transport. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 411 AA 

Protein Sequence

Gene Ontology

 GO:0071013; C:catalytic step 2 spliceosome; IEA:Compara.
 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0035145; C:exon-exon junction complex; IEA:Compara.
 GO:0016607; C:nuclear speck; IEA:UniProtKB-SubCell.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0004004; F:ATP-dependent RNA helicase activity; IEA:Compara.
 GO:0008143; F:poly(A) RNA binding; IEA:Compara.
 GO:0006397; P:mRNA processing; IEA:UniProtKB-KW.
 GO:0051028; P:mRNA transport; IEA:UniProtKB-KW.
 GO:0017148; P:negative regulation of translation; IEA:Compara.
 GO:0000184; P:nuclear-transcribed mRNA catabolic process, nonsense-mediated decay; IEA:UniProtKB-KW.
 GO:0045727; P:positive regulation of translation; IEA:Compara.
 GO:0008380; P:RNA splicing; IEA:UniProtKB-KW.
 GO:0006364; P:rRNA processing; IEA:UniProtKB-KW. 

Interpro

 IPR011545; DNA/RNA_helicase_DEAD/DEAH_N.
 IPR014001; Helicase_ATP-bd.
 IPR001650; Helicase_C.
 IPR027417; P-loop_NTPase.
 IPR000629; RNA-helicase_DEAD-box_CS.
 IPR014014; RNA_helicase_DEAD_Q_motif. 

Pfam

 PF00270; DEAD
 PF00271; Helicase_C 

SMART

 SM00487; DEXDc
 SM00490; HELICc 

PROSITE

 PS00039; DEAD_ATP_HELICASE
 PS51192; HELICASE_ATP_BIND_1
 PS51194; HELICASE_CTER
 PS51195; Q_MOTIF 

PRINTS