UniProt Accession

 PROF2_HUMAN; P35080; B2R4C8; D3DNI4; Q4VBQ4; Q8WVF9; Q9HBK2 

Genbank Protein ID

 L10678; AF228738; AK311780; AK311782; CH471052; BC002964; CH471052; BC018049; BC043646; BC095444 

Genbank Nucleotide ID

 AAA03022.1; AAG24949.1; BAG34723.1; BAG34725.1; EAW78850.1; EAW78852.1; AAH18049.1; AAH43646.1; AAH95444.1 

Protein Name

 Profilin-2 

Protein Synonyms/Alias

 Profilin II 

Gene Name

 PFN2 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
91	CTMDIRTKSQGGEPT	ubiquitination	[1, 2, 3]
127	HGGGLNKKAYSMAKY	ubiquitination	[4, 5]

Reference

 [1] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
 Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
 J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572]
 [2] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [4] Mass spectrometric analysis of lysine ubiquitylation reveals promiscuity at site level.
 Danielsen JM, Sylvestersen KB, Bekker-Jensen S, Szklarczyk D, Poulsen JW, Horn H, Jensen LJ, Mailand N, Nielsen ML.
 Mol Cell Proteomics. 2011 Mar;10(3):M110.003590. [PMID: 21139048]
 [5] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473] 

Functional Description

 Binds to actin and affects the structure of the cytoskeleton. At high concentrations, profilin prevents the polymerization of actin, whereas it enhances it at low concentrations. By binding to PIP2, it inhibits the formation of IP3 and DG. 

Sequence Annotation

 MOD_RES 99 99 Phosphotyrosine (By similarity).  

Keyword

 3D-structure; Actin-binding; Alternative splicing; Complete proteome; Cytoplasm; Cytoskeleton; Direct protein sequencing; Phosphoprotein; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 140 AA 

Protein Sequence

Gene Ontology

 GO:0015629; C:actin cytoskeleton; NAS:UniProtKB.
 GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
 GO:0043195; C:terminal bouton; IEA:Compara.
 GO:0003779; F:actin binding; NAS:UniProtKB.
 GO:0005546; F:phosphatidylinositol-4,5-bisphosphate binding; NAS:UniProtKB.
 GO:0030036; P:actin cytoskeleton organization; IEA:InterPro.
 GO:0030837; P:negative regulation of actin filament polymerization; IEA:Compara.
 GO:0030838; P:positive regulation of actin filament polymerization; IEA:Compara.
 GO:0008064; P:regulation of actin polymerization or depolymerization; NAS:UniProtKB.
 GO:2000300; P:regulation of synaptic vesicle exocytosis; IEA:Compara. 

Interpro

 IPR005454; Profilin_chordates.
 IPR027310; Profilin_CS.
 IPR005455; Profilin_eukaryotes/bac. 

Pfam

 PF00235; Profilin 

SMART

 SM00392; PROF 

PROSITE

 PS00414; PROFILIN 

PRINTS

 PR00392; PROFILIN.
 PR01639; PROFILINMAML.