UniProt Accession

 SBMC_ECOLI; P33012 

Genbank Protein ID

 X84885; U00009; U00096; AP009048 

Genbank Nucleotide ID

 CAA59312.1; AAA16415.1; AAC75070.1; BAA15830.1 

Protein Name

 DNA gyrase inhibitor 

Protein Synonyms/Alias

  

Gene Name

 sbmC 

Gene Synonyms/Alias

 gyrI; yeeB; b2009; JW1991 

Created Date

 July 27, 2013 

Organism

 Escherichia coli (strain K12) 

NCBI Taxa ID

 83333 

Lysine Modification

Position	Peptide	Type	References
27	GPWEQTVKKGFEQLM	acetylation	[1]
45	DSKNIVPKEWVAVYY	acetylation	[1]
62	PDETPAEKLRCDTVV	acetylation	[1]

Reference

 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618] 

Functional Description

 Inhibits the supercoiling activity of DNA gyrase. Acts by inhibiting DNA gyrase at an early step, prior to (or at the step of) binding of DNA by the gyrase. It protects cells against toxins that target DNA gyrase, by inhibiting activity of these toxins and reducing the formation of lethal double-strand breaks in the cell. Protects cells against the natural plasmid-encoded toxins microcin B17 (MccB17) and CcdB, and synthetic quinolones. Can also protect cells against alkylating agents that act independently of DNA gyrase, suggesting a more general role in protecting cells against DNA damage. 

Sequence Annotation

 REGION 89 96 Required for interaction with DNA gyrase  

Keyword

 3D-structure; Complete proteome; Cytoplasm; Direct protein sequencing; Reference proteome; Stress response. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 157 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0008657; F:DNA topoisomerase (ATP-hydrolyzing) inhibitor activity; IEA:InterPro.
 GO:2000372; P:negative regulation of DNA topoisomerase (ATP-hydrolyzing) activity; IEA:GOC.
 GO:2000104; P:negative regulation of DNA-dependent DNA replication; IDA:EcoCyc.
 GO:0006974; P:response to DNA damage stimulus; IEP:EcoliWiki. 

Interpro

 IPR010499; AraC_E-bd.
 IPR024911; DNA_gyrase_inhibitor_GyrI.
 IPR011256; Reg_factor_effector_bac. 

Pfam

 PF06445; GyrI-like 

SMART

 SM00871; AraC_E_bind 

PROSITE

  

PRINTS