CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-004476
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Elongation factor 3A 
Protein Synonyms/Alias
 EF-3; EF-3A; Eukaryotic elongation factor 3; eEF3; Translation elongation factor 3A; Yeast elongation factor 3 
Gene Name
 YEF3 
Gene Synonyms/Alias
 EFC1; TEF3; YEF3A; YLR249W; L9672.5 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
9SDSQQSIKVLEELFQacetylation[1]
142NAIVETNKWQEKIAIacetylation[1]
142NAIVETNKWQEKIAIubiquitination[2]
161SAMVDAAKDQVALRMubiquitination[2]
183SETMWDTKKEVKAAAacetylation[1]
183SETMWDTKKEVKAAAubiquitination[3]
187WDTKKEVKAAATAAMubiquitination[2]
196AATAAMTKATETVDNubiquitination[2]
204ATETVDNKDIERFIPacetylation[1]
204ATETVDNKDIERFIPubiquitination[2]
265RETGIKRKSAVIIDNubiquitination[2]
289VIAPFLGKLLPGLKSacetylation[1]
289VIAPFLGKLLPGLKSubiquitination[2, 3]
295GKLLPGLKSNFATIAacetylation[1]
295GKLLPGLKSNFATIAubiquitination[2]
350QVVNELLKDETVAPRubiquitination[2, 3]
401FLHEKKAKDILDEFRacetylation[1]
401FLHEKKAKDILDEFRubiquitination[2]
448GAKILLNKTQLRLKRacetylation[1]
448GAKILLNKTQLRLKRubiquitination[2]
469CGPNGCGKSTLMRAIubiquitination[2]
551LSGGWKMKLALARAVubiquitination[2]
622GLKLRKYKGNFTEFVubiquitination[2]
630GNFTEFVKKCPAAKAacetylation[1]
630GNFTEFVKKCPAAKAubiquitination[2, 3]
636VKKCPAAKAYEELSNubiquitination[2, 3]
660PGYLEGVKTKQKAIVacetylation[1]
660PGYLEGVKTKQKAIVubiquitination[2]
668TKQKAIVKVTNMEFQubiquitination[2]
681FQYPGTSKPQITDINubiquitination[2]
707IGPNGAGKSTLINVLubiquitination[2]
737NCRIAYIKQHAFAHIacetylation[1]
786NDAEAMNKIFKIEGTacetylation[1]
786NDAEAMNKIFKIEGTubiquitination[2]
789EAMNKIFKIEGTPRRacetylation[1]
789EAMNKIFKIEGTPRRubiquitination[2]
807IHSRRKFKNTYEYECubiquitination[2]
825LGENIGMKSERWVPMacetylation[1]
825LGENIGMKSERWVPMubiquitination[2]
859MVAEVDMKEALASGQacetylation[1]
859MVAEVDMKEALASGQubiquitination[2]
873QFRPLTRKEIEEHCSacetylation[1]
873QFRPLTRKEIEEHCSubiquitination[2]
902RGLSGGQKVKLVLAAubiquitination[2]
937DSLGALSKALKEFEGacetylation[1]
937DSLGALSKALKEFEGubiquitination[2]
967TEEVWAVKDGRMTPSacetylation[1]
967TEEVWAVKDGRMTPSubiquitination[2]
997KKEDEEDKFDAMGNKacetylation[1]
1004KFDAMGNKIAGGKKKacetylation[1]
1030KKERMKKKKELGDAYubiquitination[2]
Reference
 [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919]
 [2] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]
 [3] Sites of ubiquitin attachment in Saccharomyces cerevisiae.
 Starita LM, Lo RS, Eng JK, von Haller PD, Fields S.
 Proteomics. 2012 Jan;12(2):236-40. [PMID: 22106047] 
Functional Description
 Required for the ATP-dependent release of deacylated tRNA from the ribosomal E-site during protein biosynthesis. Stimulates the eEF1A-dependent binding of aminoacyl-tRNA to the ribosomal A-site, which has reduced affinity for tRNA as long as the E-site is occupied. 
Sequence Annotation
 REPEAT 5 42 HEAT 1.
 REPEAT 86 123 HEAT 2.
 REPEAT 125 162 HEAT 3.
 REPEAT 166 203 HEAT 4.
 REPEAT 205 241 HEAT 5.
 REPEAT 242 279 HEAT 6.
 REPEAT 285 323 HEAT 7.
 DOMAIN 426 641 ABC transporter 1.
 DOMAIN 667 993 ABC transporter 2.
 NP_BIND 463 470 ATP 1 (By similarity).
 NP_BIND 701 708 ATP 2 (By similarity).
 MOD_RES 2 2 N-acetylserine.
 MOD_RES 642 642 Phosphoserine.
 MOD_RES 644 644 Phosphothreonine.
 MOD_RES 972 972 Phosphothreonine.
 MOD_RES 974 974 Phosphoserine.
 MOD_RES 1015 1015 Phosphoserine.
 MOD_RES 1016 1016 Phosphoserine.
 MOD_RES 1037 1037 Phosphotyrosine.
 MOD_RES 1039 1039 Phosphoserine.
 MOD_RES 1040 1040 Phosphoserine.  
Keyword
 3D-structure; Acetylation; ATP-binding; Complete proteome; Cytoplasm; Direct protein sequencing; Elongation factor; Nucleotide-binding; Phosphoprotein; Protein biosynthesis; Reference proteome; Repeat; RNA-binding; rRNA-binding. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1044 AA 
Protein Sequence
MSDSQQSIKV LEELFQKLSV ATADNRHEIA SEVASFLNGN IIEHDVPEHF FGELAKGIKD 60
KKTAANAMQA VAHIANQSNL SPSVEPYIVQ LVPAICTNAG NKDKEIQSVA SETLISIVNA 120
VNPVAIKALL PHLTNAIVET NKWQEKIAIL AAISAMVDAA KDQVALRMPE LIPVLSETMW 180
DTKKEVKAAA TAAMTKATET VDNKDIERFI PSLIQCIADP TEVPETVHLL GATTFVAEVT 240
PATLSIMVPL LSRGLNERET GIKRKSAVII DNMCKLVEDP QVIAPFLGKL LPGLKSNFAT 300
IADPEAREVT LRALKTLRRV GNVGEDDAIP EVSHAGDVST TLQVVNELLK DETVAPRFKI 360
VVEYIAAIGA DLIDERIIDQ QAWFTHITPY MTIFLHEKKA KDILDEFRKR AVDNIPVGPN 420
FDDEEDEGED LCNCEFSLAY GAKILLNKTQ LRLKRARRYG ICGPNGCGKS TLMRAIANGQ 480
VDGFPTQEEC RTVYVEHDID GTHSDTSVLD FVFESGVGTK EAIKDKLIEF GFTDEMIAMP 540
ISALSGGWKM KLALARAVLR NADILLLDEP TNHLDTVNVA WLVNYLNTCG ITSITISHDS 600
VFLDNVCEYI INYEGLKLRK YKGNFTEFVK KCPAAKAYEE LSNTDLEFKF PEPGYLEGVK 660
TKQKAIVKVT NMEFQYPGTS KPQITDINFQ CSLSSRIAVI GPNGAGKSTL INVLTGELLP 720
TSGEVYTHEN CRIAYIKQHA FAHIESHLDK TPSEYIQWRF QTGEDRETMD RANRQINEND 780
AEAMNKIFKI EGTPRRIAGI HSRRKFKNTY EYECSFLLGE NIGMKSERWV PMMSVDNAWI 840
PRGELVESHS KMVAEVDMKE ALASGQFRPL TRKEIEEHCS MLGLDPEIVS HSRIRGLSGG 900
QKVKLVLAAG TWQRPHLIVL DEPTNYLDRD SLGALSKALK EFEGGVIIIT HSAEFTKNLT 960
EEVWAVKDGR MTPSGHNWVS GQGAGPRIEK KEDEEDKFDA MGNKIAGGKK KKKLSSAELR 1020
KKKKERMKKK KELGDAYVSS DEEF 1044 
Gene Ontology
 GO:0022626; C:cytosolic ribosome; IDA:SGD.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0016887; F:ATPase activity; IMP:SGD.
 GO:0019843; F:rRNA binding; IEA:UniProtKB-KW.
 GO:0003746; F:translation elongation factor activity; IDA:SGD.
 GO:0006415; P:translational termination; IDA:SGD. 
Interpro
 IPR003593; AAA+_ATPase.
 IPR003439; ABC_transporter-like.
 IPR017871; ABC_transporter_CS.
 IPR011989; ARM-like.
 IPR016024; ARM-type_fold.
 IPR015688; Elongation_fac_3.
 IPR021133; HEAT_type_2.
 IPR027417; P-loop_NTPase. 
Pfam
 PF00005; ABC_tran 
SMART
 SM00382; AAA 
PROSITE
 PS00211; ABC_TRANSPORTER_1
 PS50893; ABC_TRANSPORTER_2
 PS50077; HEAT_REPEAT 
PRINTS