CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-006166
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 V-type proton ATPase subunit a, vacuolar isoform 
Protein Synonyms/Alias
 V-ATPase a 1 subunit; V-ATPase 95 kDa subunit; Vacuolar pH protein 1; Vacuolar proton pump a subunit; Vacuolar proton translocating ATPase subunit a 1 
Gene Name
 VPH1 
Gene Synonyms/Alias
 YOR270C 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
135TDQIEVQKNDLEQYRacetylation[1]
135TDQIEVQKNDLEQYRubiquitination[2]
226EQPVYDVKTREYKHKubiquitination[3]
231DVKTREYKHKNAFIVubiquitination[3]
233KTREYKHKNAFIVFSubiquitination[3]
251LIIKRIRKIAESLDAubiquitination[2]
276GRSQQLAKVNKNLSDubiquitination[2]
279QQLAKVNKNLSDLYTubiquitination[2]
Reference
 [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919]
 [2] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]
 [3] Identification, analysis, and prediction of protein ubiquitination sites.
 Radivojac P, Vacic V, Haynes C, Cocklin RR, Mohan A, Heyen JW, Goebl MG, Iakoucheva LM.
 Proteins. 2010 Feb 1;78(2):365-80. [PMID: 19722269] 
Functional Description
 Subunit of the integral membrane V0 complex of vacuolar ATPase essential for assembly and catalytic activity. Is present only in vacuolar V-ATPase complexes. Enzymes containing this subunit have a 4-fold higher ratio of proton transport to ATP hydrolysis than complexes containing the Golgi/endosomal isoform and undergo reversible dissociation of V1 and V0 in response to glucose depletion. V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells. 
Sequence Annotation
 MOD_RES 2 2 N-acetylalanine.  
Keyword
 3D-structure; Acetylation; Coiled coil; Complete proteome; Direct protein sequencing; Glycoprotein; Hydrogen ion transport; Ion transport; Membrane; Reference proteome; Transmembrane; Transmembrane helix; Transport; Vacuole. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 840 AA 
Protein Sequence
MAEKEEAIFR SAEMALVQFY IPQEISRDSA YTLGQLGLVQ FRDLNSKVRA FQRTFVNEIR 60
RLDNVERQYR YFYSLLKKHD IKLYEGDTDK YLDGSGELYV PPSGSVIDDY VRNASYLEER 120
LIQMEDATDQ IEVQKNDLEQ YRFILQSGDE FFLKGDNTDS TSYMDEDMID ANGENIAAAI 180
GASVNYVTGV IARDKVATLE QILWRVLRGN LFFKTVEIEQ PVYDVKTREY KHKNAFIVFS 240
HGDLIIKRIR KIAESLDANL YDVDSSNEGR SQQLAKVNKN LSDLYTVLKT TSTTLESELY 300
AIAKELDSWF QDVTREKAIF EILNKSNYDT NRKILIAEGW IPRDELATLQ ARLGEMIARL 360
GIDVPSIIQV LDTNHTPPTF HRTNKFTAGF QSICDCYGIA QYREINAGLP TIVTFPFMFA 420
IMFGDMGHGF LMTLAALSLV LNEKKINKMK RGEIFDMAFT GRYIILLMGV FSMYTGFLYN 480
DIFSKTMTIF KSGWKWPDHW KKGESITATS VGTYPIGLDW AWHGTENALL FSNSYKMKLS 540
ILMGFIHMTY SYFFSLANHL YFNSMIDIIG NFIPGLLFMQ GIFGYLSVCI VYKWAVDWVK 600
DGKPAPGLLN MLINMFLSPG TIDDELYPHQ AKVQVFLLLM ALVCIPWLLL VKPLHFKFTH 660
KKKSHEPLPS TEADASSEDL EAQQLISAMD ADDAEEEEVG SGSHGEDFGD IMIHQVIHTI 720
EFCLNCVSHT ASYLRLWALS LAHAQLSSVL WTMTIQIAFG FRGFVGVFMT VALFAMWFAL 780
TCAVLVLMEG TSAMLHSLRL HWVESMSKFF VGEGLPYEPF AFEYKDMEVA VASASSSASS 840 
Gene Ontology
 GO:0000329; C:fungal-type vacuole membrane; IDA:UniProtKB.
 GO:0016021; C:integral to membrane; ISM:SGD.
 GO:0000220; C:vacuolar proton-transporting V-type ATPase, V0 domain; IPI:SGD.
 GO:0046961; F:proton-transporting ATPase activity, rotational mechanism; IMP:UniProtKB.
 GO:0015991; P:ATP hydrolysis coupled proton transport; IEA:InterPro.
 GO:0016049; P:cell growth; IGI:UniProtKB.
 GO:0006797; P:polyphosphate metabolic process; IMP:SGD.
 GO:0006461; P:protein complex assembly; IMP:SGD.
 GO:0007035; P:vacuolar acidification; IMP:SGD. 
Interpro
 IPR002490; V-ATPase_116kDa_su.
 IPR026028; V-type_ATPase_116kDa_su_euka. 
Pfam
 PF01496; V_ATPase_I 
SMART
  
PROSITE
  
PRINTS