UniProt Accession

 VAMP4_HUMAN; O75379; A2IDD8; Q96IY9; Q96J20; Q9UEL7 

Genbank Protein ID

 AF044310; AL035296; Z98751; CH471067; BC005974; BC007019; BC031264 

Genbank Nucleotide ID

 AAC24032.1; CAA22896.1; CAM28272.1; EAW90904.1; AAH05974.1; AAH07019.1; AAH31264.1 

Protein Name

 Vesicle-associated membrane protein 4 

Protein Synonyms/Alias

 VAMP-4 

Gene Name

 VAMP4 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
19	DDVTGSVKSERRNLL	ubiquitination	[1, 2, 3, 4, 5]
87	RLDELQDKSESLSDN	ubiquitination	[1, 4, 5]
115	QMWWRGCKIKAIMAL	ubiquitination	[4]
117	WWRGCKIKAIMALVA	ubiquitination	[4]
138	IIILIVMKYRT****	ubiquitination	[4]

Reference

 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [4] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [5] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302] 

Functional Description

 Involved in the pathway that functions to remove an inhibitor (probably synaptotagmin-4) of calcium-triggered exocytosis during the maturation of secretory granules. May be a marker for this sorting pathway that is critical for remodeling the secretory response of granule. 

Sequence Annotation

 DOMAIN 52 112 v-SNARE coiled-coil homology.
 MOD_RES 17 17 Phosphoserine (By similarity).
 MOD_RES 30 30 Phosphoserine.  

Keyword

 Alternative splicing; Coiled coil; Complete proteome; Golgi apparatus; Membrane; Phosphoprotein; Reference proteome; Transmembrane; Transmembrane helix. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 141 AA 

Protein Sequence

Gene Ontology

 GO:0005768; C:endosome; TAS:ProtInc.
 GO:0000139; C:Golgi membrane; TAS:ProtInc.
 GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
 GO:0005764; C:lysosome; TAS:ProtInc.
 GO:0031201; C:SNARE complex; IEA:Compara.
 GO:0005802; C:trans-Golgi network; IEA:Compara.
 GO:0043001; P:Golgi to plasma membrane protein transport; IEA:Compara.
 GO:0042996; P:regulation of Golgi to plasma membrane protein transport; IEA:Compara. 

Interpro

 IPR001388; Synaptobrevin.
 IPR016444; Synaptobrevin_met/fun. 

Pfam

 PF00957; Synaptobrevin 

SMART

  

PROSITE

 PS00417; SYNAPTOBREVIN
 PS50892; V_SNARE 

PRINTS

 PR00219; SYNAPTOBREVN.