| Tag | Content |
|---|
| CPLM ID | CPLM-004751 |
UniProt Accession | |
Genbank Protein ID | |
Genbank Nucleotide ID | |
Protein Name | Neurofilament heavy polypeptide |
Protein Synonyms/Alias | NF-H; 200 kDa neurofilament protein; Neurofilament triplet H protein |
Gene Name | Nefh |
Gene Synonyms/Alias | Kiaa0845; Nfh |
Created Date | July 27, 2013 |
Organism | Mus musculus (Mouse) |
NCBI Taxa ID | 10090 |
Lysine Modification | Position | Peptide | Type | References |
|---|
| 96 | AVAARSEKEQLQALN | ubiquitination | [1] | | 262 | AEARDALKCDVTSAL | ubiquitination | [1] | | 303 | DRLSEAAKVNTDAMR | ubiquitination | [1] | | 402 | IEIAAYRKLLEGEEC | ubiquitination | [1] | | 443 | VKSEEMIKVVEKSEK | ubiquitination | [1] |
|
Reference | [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues. Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C. Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [ PMID: 22790023] |
Functional Description | Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. NF-H has an important function in mature axons that is not subserved by the two smaller NF proteins. |
Sequence Annotation | REPEAT 522 527 1.
REPEAT 528 533 2.
REPEAT 534 539 3.
REPEAT 540 545 4.
REPEAT 546 551 5.
REPEAT 552 557 6.
REPEAT 558 563 7.
REPEAT 564 569 8.
REPEAT 570 575 9.
REPEAT 576 581 10.
REPEAT 582 587 11.
REPEAT 588 593 12.
REPEAT 594 599 13.
REPEAT 600 605 14.
REPEAT 606 611 15.
REPEAT 612 617 16.
REPEAT 618 623 17.
REPEAT 624 629 18.
REPEAT 630 635 19.
REPEAT 636 641 20.
REPEAT 642 647 21.
REPEAT 648 653 22.
REPEAT 654 659 23.
REPEAT 660 665 24.
REPEAT 666 671 25.
REPEAT 672 677 26.
REPEAT 678 683 27.
REPEAT 684 689 28.
REPEAT 690 695 29.
REPEAT 696 701 30.
REPEAT 702 707 31.
REPEAT 708 713 32.
REPEAT 714 719 33.
REPEAT 720 725 34.
REPEAT 726 731 35.
REPEAT 732 737 36.
REPEAT 738 743 37.
REPEAT 744 749 38.
REPEAT 750 755 39.
REPEAT 756 761 40.
REPEAT 762 767 41.
REPEAT 768 773 42.
REPEAT 774 779 43; approximate.
REPEAT 782 787 44.
REPEAT 788 793 45.
REPEAT 794 799 46.
REPEAT 808 813 47.
REPEAT 814 819 48.
REPEAT 833 838 49.
REPEAT 858 863 50.
REPEAT 866 871 51.
REPEAT 887 892 52.
REGION 2 98 Head.
REGION 99 411 Rod.
REGION 99 130 Coil 1A.
REGION 131 143 Linker 1.
REGION 144 242 Coil 1B.
REGION 243 264 Linker 12.
REGION 265 286 Coil 2A.
REGION 287 290 Linker 2.
REGION 291 411 Coil 2B.
REGION 412 1090 Tail.
REGION 522 892 52 X 6 AA approximate tandem repeats of
MOD_RES 61 61 Phosphoserine.
MOD_RES 416 416 Phosphoserine.
MOD_RES 499 499 Phosphothreonine.
MOD_RES 500 500 Phosphoserine.
MOD_RES 523 523 Phosphoserine.
MOD_RES 529 529 Phosphoserine.
MOD_RES 535 535 Phosphoserine.
MOD_RES 541 541 Phosphoserine.
MOD_RES 547 547 Phosphoserine.
MOD_RES 553 553 Phosphoserine.
MOD_RES 559 559 Phosphoserine.
MOD_RES 565 565 Phosphoserine.
MOD_RES 571 571 Phosphoserine.
MOD_RES 577 577 Phosphoserine.
MOD_RES 583 583 Phosphoserine.
MOD_RES 613 613 Phosphoserine.
MOD_RES 619 619 Phosphoserine.
MOD_RES 625 625 Phosphoserine.
MOD_RES 631 631 Phosphoserine.
MOD_RES 637 637 Phosphoserine.
MOD_RES 649 649 Phosphoserine.
MOD_RES 655 655 Phosphoserine.
MOD_RES 661 661 Phosphoserine.
MOD_RES 667 667 Phosphoserine.
MOD_RES 673 673 Phosphoserine.
MOD_RES 679 679 Phosphoserine.
MOD_RES 685 685 Phosphoserine.
MOD_RES 691 691 Phosphoserine.
MOD_RES 697 697 Phosphoserine.
MOD_RES 703 703 Phosphoserine.
MOD_RES 709 709 Phosphoserine.
MOD_RES 715 715 Phosphoserine.
MOD_RES 721 721 Phosphoserine.
MOD_RES 727 727 Phosphoserine.
MOD_RES 733 733 Phosphoserine.
MOD_RES 739 739 Phosphoserine.
MOD_RES 745 745 Phosphoserine.
MOD_RES 751 751 Phosphoserine.
MOD_RES 757 757 Phosphoserine.
MOD_RES 763 763 Phosphoserine.
MOD_RES 769 769 Phosphoserine.
MOD_RES 775 775 Phosphothreonine.
MOD_RES 783 783 Phosphoserine.
MOD_RES 789 789 Phosphoserine.
MOD_RES 815 815 Phosphoserine.
MOD_RES 834 834 Phosphoserine.
MOD_RES 839 839 Phosphothreonine.
MOD_RES 867 867 Phosphoserine.
MOD_RES 888 888 Phosphoserine. |
Keyword | Coiled coil; Complete proteome; Direct protein sequencing; Intermediate filament; Phosphoprotein; Reference proteome; Repeat. |
Sequence Source | UniProt (SWISSPROT/TrEMBL); GenBank; EMBL |
Protein Length | 1090 AA |
Protein Sequence | MMSFGSADAL LGAPFAPLHG GGSLHYSLSR KAGPGGTRSA AGSSSGFHSW ARTSVSSVSA 60
SPSRFRGAAS STDSLDTLSN GPEGCVVAAV AARSEKEQLQ ALNDRFAGYI DKVRQLEAHN 120
RSLEGEAAAL RQQQAGRAAM GELYEREVRE MRGAVLRLGA ARGQLRLEQE HLLEDIAHVR 180
QRLDEEARQR EEAEAAARAL ARFAQEAEAA RVELQKKAQA LQEECGYLRR HHQEEVGELL 240
GQIQGCGAAQ AQAQAEARDA LKCDVTSALR EIRAQLEGHA VQSTLQSEEW FRVRLDRLSE 300
AAKVNTDAMR SAQEEITEYR RQLQARTTEL EALKSTKESL ERQRSELEDR HQADIASYQD 360
AIQQLDSELR NTKWEMAAQL REYQDLLNVK MALDIEIAAY RKLLEGEECR IGFGPSPFSL 420
TEGLPKIPSI STHIKVKSEE MIKVVEKSEK ETVIVEGQTE EIRVTEGVTE EEDKEAQGQE 480
GEEAEEGEEK EEEEGAAATS PPAEEAASPE KETKSRVKEE AKSPGEAKSP GEAKSPAEAK 540
SPGEAKSPGE AKSPGEAKSP AEPKSPAEPK SPAEAKSPAE PKSPATVKSP GEAKSPSEAK 600
SPAEAKSPAE AKSPAEAKSP AEAKSPAEAK SPAEAKSPAT VKSPGEAKSP SEAKSPAEAK 660
SPAEAKSPAE AKSPAEVKSP GEAKSPAEPK SPAEAKSPAE VKSPAEAKSP AEVKSPGEAK 720
SPAAVKSPAE AKSPAAVKSP GEAKSPGEAK SPAEAKSPAE AKSPIEVKSP EKAKTPVKEG 780
AKSPAEAKSP EKAKSPVKED IKPPAEAKSP EKAKSPVKEG AKPPEKAKPL DVKSPEAQTP 840
VQEEAKHPTD IRPPEQVKSP AKEKAKSPEK EEAKTSEKVA PKKEEVKSPV KEEVKAKEPP 900
KKVEEEKTLP TPKTEAKESK KDEAPKEAPK PKVEEKKETP TEKPKDSTAE AKKEEAGEKK 960
KAVASEEETP AKLGVKEEAK PKEKTETTKT EAEDTKAKEP SKPTETEKPK KEEMPAAPEK 1020
KDTKEEKTTE SRKPEEKPKM EAKVKEDDKS LSKEPSKPKT EKAEKSSSTD QKESQPPEKT 1080
TEDKATKGEK 1090 |
Gene Ontology | GO:0005739; C:mitochondrion; IDA:MGI. GO:0005883; C:neurofilament; IDA:MGI. GO:0045735; F:nutrient reservoir activity; IEA:InterPro. GO:0045110; P:intermediate filament bundle assembly; IGI:MGI. GO:0000226; P:microtubule cytoskeleton organization; IGI:MGI. GO:0060052; P:neurofilament cytoskeleton organization; IMP:MGI. GO:0048936; P:peripheral nervous system neuron axonogenesis; IMP:MGI. |
Interpro | |
Pfam | |
SMART | |
PROSITE | |
PRINTS | |