CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-023010
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 CCR4-NOT transcription complex subunit 7 
Protein Synonyms/Alias
 BTG1-binding factor 1; CCR4-associated factor 1; CAF-1; Caf1a 
Gene Name
 CNOT7 
Gene Synonyms/Alias
 CAF1 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
33KIRQVIRKYNYVAMDubiquitination[1]
206MKSCKNLKGGLQEVAubiquitination[1, 2, 3]
240LTGMAFFKMREMFFEubiquitination[1, 4]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [4] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094
Functional Description
 Has 3'-5' poly(A) exoribonuclease activity for synthetic poly(A) RNA substrate. Its function seems to be partially redundant with that of CNOT8. Catalytic component of the CCR4-NOT complex which is one of the major cellular mRNA deadenylases and is linked to various cellular processes including bulk mRNA degradation, miRNA-mediated repression, translational repression during translational initiation and general transcription regulation. During miRNA-mediated repression the complex seems also to act as translational repressor during translational initiation. Additional complex functions may be a consequence of its influence on mRNA expression. Associates with members of the BTG family such as TOB1 and BTG2 and is required for their anti- proliferative activity. 
Sequence Annotation
 METAL 40 40 Divalent metal cation 1; catalytic
 METAL 40 40 Divalent metal cation 2; catalytic
 METAL 42 42 Divalent metal cation 2; catalytic
 METAL 161 161 Divalent metal cation 1; catalytic
 METAL 230 230 Divalent metal cation 2; catalytic
 METAL 278 278 Divalent metal cation 1; catalytic  
Keyword
 3D-structure; Alternative splicing; Complete proteome; Cytoplasm; Exonuclease; Hydrolase; Magnesium; Metal-binding; Nuclease; Nucleus; Reference proteome; Repressor; RNA-binding; RNA-mediated gene silencing; Transcription; Transcription regulation; Translation regulation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 285 AA 
Protein Sequence
MPAATVDHSQ RICEVWACNL DEEMKKIRQV IRKYNYVAMD TEFPGVVARP IGEFRSNADY 60
QYQLLRCNVD LLKIIQLGLT FMNEQGEYPP GTSTWQFNFK FNLTEDMYAQ DSIELLTTSG 120
IQFKKHEEEG IETQYFAELL MTSGVVLCEG VKWLSFHSGY DFGYLIKILT NSNLPEEELD 180
FFEILRLFFP VIYDVKYLMK SCKNLKGGLQ EVAEQLELER IGPQHQAGSD SLLTGMAFFK 240
MREMFFEDHI DDAKYCGHLY GLGSGSSYVQ NGTGNAYEEE ANKQS 285 
Gene Ontology
 GO:0030014; C:CCR4-NOT complex; IDA:UniProtKB.
 GO:0000932; C:cytoplasmic mRNA processing body; ISS:UniProtKB.
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0005634; C:nucleus; IDA:UniProtKB.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0004535; F:poly(A)-specific ribonuclease activity; IDA:UniProtKB.
 GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
 GO:0003700; F:sequence-specific DNA binding transcription factor activity; TAS:ProtInc.
 GO:0004871; F:signal transducer activity; TAS:ProtInc.
 GO:0005975; P:carbohydrate metabolic process; TAS:ProtInc.
 GO:0033962; P:cytoplasmic mRNA processing body assembly; IEA:Compara.
 GO:0000290; P:deadenylation-dependent decapping of nuclear-transcribed mRNA; IEA:Compara.
 GO:0043928; P:exonucleolytic nuclear-transcribed mRNA catabolic process involved in deadenylation-dependent decay; IDA:UniProtKB.
 GO:0035195; P:gene silencing by miRNA; TAS:UniProtKB.
 GO:0008285; P:negative regulation of cell proliferation; IDA:UniProtKB.
 GO:0000289; P:nuclear-transcribed mRNA poly(A) tail shortening; TAS:Reactome.
 GO:0008284; P:positive regulation of cell proliferation; IMP:UniProtKB.
 GO:1900153; P:positive regulation of nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay; IMP:UniProtKB.
 GO:0060213; P:positive regulation of nuclear-transcribed mRNA poly(A) tail shortening; IMP:UniProtKB.
 GO:0045944; P:positive regulation of transcription from RNA polymerase II promoter; IEA:Compara.
 GO:0006417; P:regulation of translation; IEA:UniProtKB-KW.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 
Interpro
 IPR006941; RNase_CAF1.
 IPR012337; RNaseH-like_dom. 
Pfam
 PF04857; CAF1 
SMART
  
PROSITE
  
PRINTS