CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-005439
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 DnaJ homolog subfamily B member 2 
Protein Synonyms/Alias
 DnaJ protein homolog 1; Heat shock 40 kDa protein 3; Heat shock protein J1; HSJ-1 
Gene Name
 DNAJB2 
Gene Synonyms/Alias
 HSJ1; HSPF3 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
20SASADDIKKAYRRKAubiquitination[1]
21ASADDIKKAYRRKALubiquitination[2]
34ALQWHPDKNPDNKEFubiquitination[1, 2, 3, 4]
39PDKNPDNKEFAEKKFubiquitination[3, 4]
47EFAEKKFKEVAEAYEubiquitination[2]
59AYEVLSDKHKREIYDubiquitination[2, 3, 4]
61EVLSDKHKREIYDRYubiquitination[1, 2]
288HRRQGRPKAQHQDPGubiquitination[2, 5]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [4] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [5] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302] 
Functional Description
  
Sequence Annotation
 DOMAIN 1 71 J.
 REPEAT 210 226 UIM 1.
 REPEAT 250 269 UIM 2.  
Keyword
 3D-structure; Alternative splicing; Chaperone; Complete proteome; Neurodegeneration; Polymorphism; Reference proteome; Repeat. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 324 AA 
Protein Sequence
MASYYEILDV PRSASADDIK KAYRRKALQW HPDKNPDNKE FAEKKFKEVA EAYEVLSDKH 60
KREIYDRYGR EGLTGTGTGP SRAEAGSGGP GFTFTFRSPE EVFREFFGSG DPFAELFDDL 120
GPFSELQNRG SRHSGPFFTF SSSFPGHSDF SSSSFSFSPG AGAFRSVSTS TTFVQGRRIT 180
TRRIMENGQE RVEVEEDGQL KSVTINGVPD DLALGLELSR REQQPSVTSR SGGTQVQQTP 240
ASCPLDSDLS EDEDLQLAMA YSLSEMEAAG KKPAGGREAQ HRRQGRPKAQ HQDPGLGGTQ 300
EGARGEATKR SPSPEEKASR CLIL 324 
Gene Ontology
 GO:0016234; C:inclusion body; IDA:BHF-UCL.
 GO:0031593; F:polyubiquitin binding; IDA:BHF-UCL.
 GO:0070628; F:proteasome binding; IDA:BHF-UCL.
 GO:0051082; F:unfolded protein binding; TAS:ProtInc.
 GO:0008219; P:cell death; IEA:UniProtKB-KW.
 GO:0030433; P:ER-associated protein catabolic process; IDA:BHF-UCL.
 GO:0030308; P:negative regulation of cell growth; IGI:UniProtKB.
 GO:0008285; P:negative regulation of cell proliferation; IGI:UniProtKB.
 GO:0090084; P:negative regulation of inclusion body assembly; IDA:BHF-UCL.
 GO:0090086; P:negative regulation of protein deubiquitination; IDA:BHF-UCL.
 GO:0032436; P:positive regulation of proteasomal ubiquitin-dependent protein catabolic process; IDA:BHF-UCL.
 GO:0031398; P:positive regulation of protein ubiquitination; IDA:BHF-UCL.
 GO:0006457; P:protein folding; TAS:ProtInc.
 GO:0006986; P:response to unfolded protein; TAS:ProtInc. 
Interpro
 IPR001623; DnaJ_domain.
 IPR018253; DnaJ_domain_CS.
 IPR003903; Ubiquitin-int_motif. 
Pfam
 PF00226; DnaJ 
SMART
 SM00271; DnaJ
 SM00726; UIM 
PROSITE
 PS00636; DNAJ_1
 PS50076; DNAJ_2
 PS50330; UIM 
PRINTS
 PR00625; JDOMAIN.