UniProt Accession

 ARNA_ECOLI; P77398; Q56VX0 

Genbank Protein ID

 AY057445; U00096; AP009048 

Genbank Nucleotide ID

 AAL23678.1; AAC75315.1; BAA16078.1 

Protein Name

 Bifunctional polymyxin resistance protein ArnA 

Protein Synonyms/Alias

 Polymyxin resistance protein PmrI; UDP-4-amino-4-deoxy-L-arabinose formyltransferase; ArnAFT; UDP-L-Ara4N formyltransferase; UDP-glucuronic acid oxidase, UDP-4-keto-hexauronic acid decarboxylating; ArnADH; UDP-GlcUA decarboxylase; UDP-glucuronic acid dehydrogenase 

Gene Name

 arnA 

Gene Synonyms/Alias

 pmrI; yfbG; b2255; JW2249 

Created Date

 July 27, 2013 

Organism

 Escherichia coli (strain K12) 

NCBI Taxa ID

 83333 

Lysine Modification

Position	Peptide	Type	References
164	IAITLHHKLCHAARQ	acetylation	[1]
181	EQTLPAIKHGNILEI	acetylation	[1]
212	DSFLEWHKPASVLHN	acetylation	[1]
442	VYGMCSDKYFDEDHS	acetylation	[1]
458	LIVGPVNKPRWIYSV	acetylation	[1]
526	LVEGSPIKLIDGGKQ	acetylation	[1]
611	ESSSYYGKGYQDVEH	acetylation	[1]

Reference

 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618] 

Functional Description

 Bifunctional enzyme that catalyzes the oxidative decarboxylation of UDP-glucuronic acid (UDP-GlcUA) to UDP-4-keto- arabinose (UDP-Ara4O) and the addition of a formyl group to UDP-4- amino-4-deoxy-L-arabinose (UDP-L-Ara4N) to form UDP-L-4-formamido- arabinose (UDP-L-Ara4FN). The modified arabinose is attached to lipid A and is required for resistance to polymyxin and cationic antimicrobial peptides. 

Sequence Annotation

 NP_BIND 368 369 NAD binding.
 REGION 1 304 Formyltransferase ArnAFT.
 REGION 86 88 10-formyltetrahydrofolate binding.
 REGION 136 140 10-formyltetrahydrofolate binding.
 REGION 314 660 Dehydrogenase ArnADH.
 REGION 432 433 UDP-glucuronate binding.
 REGION 526 535 UDP-glucuronate binding.
 ACT_SITE 104 104 Proton donor; for formyltransferase
 ACT_SITE 434 434 Proton acceptor; for decarboxylase
 ACT_SITE 619 619 Proton donor; for decarboxylase activity.
 BINDING 114 114 10-formyltetrahydrofolate.
 BINDING 347 347 NAD.
 BINDING 393 393 UDP-glucuronate; via carbonyl oxygen.
 BINDING 398 398 UDP-glucuronate.
 BINDING 460 460 UDP-glucuronate.
 BINDING 492 492 UDP-glucuronate.
 BINDING 613 613 UDP-glucuronate.  

Keyword

 3D-structure; Antibiotic resistance; Complete proteome; Lipid A biosynthesis; Lipid biosynthesis; Lipid metabolism; Lipopolysaccharide biosynthesis; Multifunctional enzyme; NAD; Oxidoreductase; Reference proteome; Transferase. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 660 AA 

Protein Sequence

Gene Ontology

 GO:0050662; F:coenzyme binding; IEA:InterPro.
 GO:0016742; F:hydroxymethyl-, formyl- and related transferase activity; IDA:EcoCyc.
 GO:0016616; F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor; IDA:EcoCyc.
 GO:0009245; P:lipid A biosynthetic process; IDA:EcoCyc.
 GO:0009103; P:lipopolysaccharide biosynthetic process; IEA:UniProtKB-UniPathway.
 GO:0046677; P:response to antibiotic; IDA:EcoCyc. 

Interpro

 IPR021168; Bifun_polymyxin_resist_ArnA.
 IPR001509; Epimerase_deHydtase.
 IPR005793; Formyl_trans_C.
 IPR002376; Formyl_transf_N.
 IPR011034; Formyl_transferase_C-like.
 IPR016040; NAD(P)-bd_dom. 

Pfam

 PF01370; Epimerase
 PF02911; Formyl_trans_C
 PF00551; Formyl_trans_N 

SMART

  

PROSITE

  

PRINTS