CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-023058
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Anaphase-promoting complex subunit 4 
Protein Synonyms/Alias
 APC4; Cyclosome subunit 4 
Gene Name
 ANAPC4 
Gene Synonyms/Alias
 APC4 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
468DLYNRKGKYFNVERVubiquitination[1, 2]
772SNKPVKIKEEVLSESsumoylation[3]
798LAPEIVIKVEKLDPEsumoylation[3]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [3] Site-specific identification of SUMO-2 targets in cells reveals an inverted SUMOylation motif and a hydrophobic cluster SUMOylation motif.
 Matic I, Schimmel J, Hendriks IA, van Santen MA, van de Rijke F, van Dam H, Gnad F, Mann M, Vertegaal AC.
 Mol Cell. 2010 Aug 27;39(4):641-52. [PMID: 20797634] 
Functional Description
 Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. 
Sequence Annotation
 MOD_RES 469 469 Phosphotyrosine.
 MOD_RES 757 757 Phosphoserine.
 MOD_RES 758 758 Phosphoserine.
 MOD_RES 777 777 Phosphoserine.
 MOD_RES 779 779 Phosphoserine.  
Keyword
 Alternative splicing; Cell cycle; Cell division; Complete proteome; Mitosis; Phosphoprotein; Polymorphism; Reference proteome; Ubl conjugation pathway. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 808 AA 
Protein Sequence
MLRFPTCFPS FRVVGEKQLP QEIIFLVWSP KRDLIALANT AGEVLLHRLA SFHRVWSFPP 60
NENTGKEVTC LAWRPDGKLL AFALADTKKI VLCDVEKPES LHSFSVEAPV SCMHWMEVTV 120
ESSVLTSFYN AEDESNLLLP KLPTLPKNYS NTSKIFSEEN SDEIIKLLGD VRLNILVLGG 180
SSGFIELYAY GMFKIARVTG IAGTCLALCL SSDLKSLSVV TEVSTNGASE VSYFQLETNL 240
LYSFLPEVTR MARKFTHISA LLQYINLSLT CMCEAWEEIL MQMDSRLTKF VQEKNTTTSV 300
QDEFMHLLLW GKASAELQTL LMNQLTVKGL KKLGQSIESS YSSIQKLVIS HLQSGSESLL 360
YHLSELKGMA SWKQKYEPLG LDAAGIEEAI TAVGSFILKA NELLQVIDSS MKNFKAFFRW 420
LYVAMLRMTE DHVLPELNKM TQKDITFVAE FLTEHFNEAP DLYNRKGKYF NVERVGQYLK 480
DEDDDLVSPP NTEGNQWYDF LQNSSHLKES PLLFPYYPRK SLHFVKRRME NIIDQCLQKP 540
ADVIGKSMNQ AICIPLYRDT RSEDSTRRLF KFPFLWNNKT SNLHYLLFTI LEDSLYKMCI 600
LRRHTDISQS VSNGLIAIKF GSFTYATTEK VRRSIYSCLD AQFYDDETVT VVLKDTVGRE 660
GRDRLLVQLP LSLVYNSEDS AEYQFTGTYS TRLDEQCSAI PTRTMHFEKH WRLLESMKAQ 720
YVAGNGFRKV SCVLSSNLRH VRVFEMDIDD EWELDESSDE EEEASNKPVK IKEEVLSESE 780
AENQQAGAAA LAPEIVIKVE KLDPELDS 808 
Gene Ontology
 GO:0005680; C:anaphase-promoting complex; IDA:UniProtKB.
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0005654; C:nucleoplasm; TAS:Reactome.
 GO:0004842; F:ubiquitin-protein ligase activity; TAS:ProtInc.
 GO:0031145; P:anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; TAS:Reactome.
 GO:0051301; P:cell division; IEA:UniProtKB-KW.
 GO:0000086; P:G2/M transition of mitotic cell cycle; TAS:ProtInc.
 GO:0000090; P:mitotic anaphase; TAS:ProtInc.
 GO:0007094; P:mitotic spindle assembly checkpoint; TAS:Reactome.
 GO:0051436; P:negative regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle; TAS:Reactome.
 GO:0051437; P:positive regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle; TAS:Reactome.
 GO:0070979; P:protein K11-linked ubiquitination; IDA:UniProtKB. 
Interpro
 IPR024789; APC4.
 IPR024790; APC4_long_dom.
 IPR017169; APC4_metazoa.
 IPR024977; Apc4_WD40_dom.
 IPR015943; WD40/YVTN_repeat-like_dom.
 IPR017986; WD40_repeat_dom. 
Pfam
 PF12896; Apc4
 PF12894; Apc4_WD40 
SMART
  
PROSITE
  
PRINTS