CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-002467
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Histidine--tRNA ligase, mitochondrial 
Protein Synonyms/Alias
 Histidyl-tRNA synthetase; HisRS 
Gene Name
 HTS1 
Gene Synonyms/Alias
 YPR033C; YP9367.13C 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
105LREILAGKYGEDSKLacetylation[1]
472KAKANPRKQFDAAEKubiquitination[2]
527IVPIVQEKLSQIHEDubiquitination[2]
Reference
 [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919]
 [2] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301] 
Functional Description
 Catalyzes the aminoacylation of histidyl-tRNA in both the cytoplasm and the mitochondrion. 
Sequence Annotation
  
Keyword
 Alternative initiation; Aminoacyl-tRNA synthetase; ATP-binding; Complete proteome; Cytoplasm; Ligase; Mitochondrion; Nucleotide-binding; Protein biosynthesis; Reference proteome; Transit peptide. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 546 AA 
Protein Sequence
MLSRSLNKVV TSIKSSSIIR MSSATAAATS APTANAANAL KASKAPKKGK LQVSLKTPKG 60
TKDWADSDMV IREAIFSTLS GLFKKHGGVT IDTPVFELRE ILAGKYGEDS KLIYNLEDQG 120
GELCSLRYDL TVPFARYVAM NNIQSIKRYH IAKVYRRDQP AMTKGRMREF YQCDFDVAGT 180
FESMVPDSEC LSILVEGLTS LGIKDFKIKL NHRKILDGIF QIAGVKDEDV RKISSAVDKL 240
DKSPWEAVKK EMTEEKGQSE ETADKIGEYV KLNGSLKEIH AVLSADANIT SNEKAKQGLD 300
DIATLMKYTE AFDIDSFISF DLSLARGLDY YTGLIYEVVT SASAPPENAS ELKKKAKSAE 360
DASEFVGVGS IAAGGRYDNL VNMFSEASGK KSTQIPCVGI SFGVERIFSL IKQRINSSTT 420
IKPTATQVFV MAFGGGKDWT GYLPERMKVT KQLWDAGIEA EYVYKAKANP RKQFDAAEKA 480
GCHIAVILGK EEYLEGKLRV KRLGQEFADD DGELVSAADI VPIVQEKLSQ IHEDGLNEVT 540
RLIKGL 546 
Gene Ontology
 GO:0005739; C:mitochondrion; IMP:SGD.
 GO:0004812; F:aminoacyl-tRNA ligase activity; IDA:SGD.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0004821; F:histidine-tRNA ligase activity; IEA:EC.
 GO:0006427; P:histidyl-tRNA aminoacylation; IDA:SGD.
 GO:0032543; P:mitochondrial translation; IGI:SGD. 
Interpro
 IPR002314; aa-tRNA-synt_IIb_cons-dom.
 IPR006195; aa-tRNA-synth_II.
 IPR004154; Anticodon-bd.
 IPR015807; His-tRNA-ligase.
 IPR004516; HisRS/HisZ. 
Pfam
 PF03129; HGTP_anticodon
 PF00587; tRNA-synt_2b 
SMART
  
PROSITE
 PS50862; AA_TRNA_LIGASE_II 
PRINTS