UniProt Accession

 RPC8_HUMAN; Q9Y535; B0QYH9; Q5M7Y8; Q96AE3; Q9BY95 

Genbank Protein ID

 AY092087; AB051452; CR456459; AL023553; AL023553; CH471095; BC017248; BC088367 

Genbank Nucleotide ID

 AAM18217.1; BAB33335.1; CAG30345.1; CAB46023.1; CAQ10207.1; EAW60438.1; AAH17248.1; AAH88367.1 

Protein Name

 DNA-directed RNA polymerase III subunit RPC8 

Protein Synonyms/Alias

 RNA polymerase III subunit C8; DNA-directed RNA polymerase III subunit H; RNA polymerase III subunit 22.9 kDa subunit; RPC22.9 

Gene Name

 POLR3H 

Gene Synonyms/Alias

 KIAA1665; RPC8 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
21	PPWQFERKLNDSIAE	ubiquitination	[1]
32	SIAEELNKKLANKVV	ubiquitination	[1]
90	EILIGKIKGCSPEGV	ubiquitination	[1]
179	TSSEELPKKEAPYTL	ubiquitination	[2]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983] 

Functional Description

 DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific peripheric component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF- Kappa-B through the RIG-I pathway (By similarity). 

Sequence Annotation

  

Keyword

 Alternative splicing; Antiviral defense; Complete proteome; DNA-directed RNA polymerase; Immunity; Innate immunity; Nucleus; Reference proteome; Transcription. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 204 AA 

Protein Sequence

Gene Ontology

 GO:0005813; C:centrosome; IDA:HPA.
 GO:0005666; C:DNA-directed RNA polymerase III complex; IDA:UniProtKB.
 GO:0003677; F:DNA binding; IEA:InterPro.
 GO:0003899; F:DNA-directed RNA polymerase activity; IDA:UniProtKB.
 GO:0051607; P:defense response to virus; IEA:UniProtKB-KW.
 GO:0045087; P:innate immune response; IEA:UniProtKB-KW.
 GO:0006386; P:termination of RNA polymerase III transcription; TAS:Reactome.
 GO:0006385; P:transcription elongation from RNA polymerase III promoter; TAS:Reactome. 

Interpro

 IPR012340; NA-bd_OB-fold.
 IPR013238; RNA_pol_III_Rbc25.
 IPR005576; RNA_pol_Rpb7_N.
 IPR004519; RNAP_E/RPC8. 

Pfam

 PF08292; RNA_pol_Rbc25
 PF03876; SHS2_Rpb7-N 

SMART

  

PROSITE

  

PRINTS