UniProt Accession

 E3_VACCW; P21605; Q76ZW2 

Genbank Protein ID

 M36339; AY243312 

Genbank Nucleotide ID

 AAB59823.1; AAO89338.1 

Protein Name

 Protein E3 

Protein Synonyms/Alias

 p25 

Gene Name

 VACWR059; E3L 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Vaccinia virus (strain Western Reserve) (VACV) (Vaccinia virus (strai 

NCBI Taxa ID

 10254 

Lysine Modification

Position	Peptide	Type	References
40	TRQLNMEKREVNKAL	sumoylation	[1]
99	KSMREDHKSFDDVIP	sumoylation	[1]

Reference

 [1] Regulation of vaccinia virus E3 protein by small ubiquitin-like modifier proteins.
 González-Santamaría J, Campagna M, García MA, Marcos-Villar L, González D, Gallego P, Lopitz-Otsoa F, Guerra S, Rodríguez MS, Esteban M, Rivas C.
 J Virol. 2011 Dec;85(24):12890-900. [PMID: 21957283] 

Functional Description

 The C-terminus binds to and sequesters double-stranded RNA (dsRNA) synthesized during viral infection. This binding acts to 'mask' the dsRNA thereby preventing recognition and subsequent activation of EIF2AK2/PKR. The N-terminus is required for phosphorylation regulation of the translation initiation factor EIF2S1, but without affecting cytosolic protein translation. Blocks the phosphorylation and subsequent activation of IRF3 and IRF7 kinases, that are required for interferon-alpha (IFN-alpha) gene expression. Also inhibits NF-kappa-B activation and the ubiquitin-like protein G1P2/ISG15, which is an early antiviral protein (By similarity). 

Sequence Annotation

 REPEAT 5 73 DRADA.
 DOMAIN 117 184 DRBM.  

Keyword

 Alternative initiation; Complete proteome; Direct protein sequencing; Host-virus interaction; Inhibition of host IFN-mediated response initiation by virus; Inhibition of host innate immune response by virus; Inhibition of host interferon signaling pathway by virus; Inhibition of host IRF3 by virus; Inhibition of host IRF7 by virus; Inhibition of host ISG15 by virus; Inhibition of host PKR by virus; Reference proteome; RNA-binding; Viral immunoevasion. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 190 AA 

Protein Sequence

Gene Ontology

 GO:0003726; F:double-stranded RNA adenosine deaminase activity; IEA:InterPro.
 GO:0003725; F:double-stranded RNA binding; IEA:InterPro.
 GO:0039548; P:suppression by virus of host IRF3 activity; IEA:UniProtKB-KW.
 GO:0039557; P:suppression by virus of host IRF7 activity; IEA:UniProtKB-KW.
 GO:0039579; P:suppression by virus of host ISG15 activity; IEA:UniProtKB-KW.
 GO:0039580; P:suppression by virus of host PKR activity; IEA:UniProtKB-KW.
 GO:0039501; P:suppression by virus of host type I interferon production; IEA:UniProtKB-KW.
 GO:0039502; P:suppression by virus of host type I interferon-mediated signaling pathway; IEA:UniProtKB-KW. 

Interpro

 IPR001159; Ds-RNA-bd.
 IPR014720; dsRNA-bd-like_dom.
 IPR000607; dsRNA_A_deaminase.
 IPR009179; Interferon-R_VACE3L.
 IPR011991; WHTH_DNA-bd_dom. 

Pfam

 PF00035; dsrm
 PF02295; z-alpha 

SMART

 SM00358; DSRM
 SM00550; Zalpha 

PROSITE

 PS50139; DRADA_REPEAT
 PS50137; DS_RBD 

PRINTS