CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-020187
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 tRNA-specific adenosine deaminase 1 
Protein Synonyms/Alias
 hADAT1; tRNA-specific adenosine-37 deaminase 
Gene Name
 ADAT1 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
69VSMGTGTKCIGQSKMubiquitination[1]
252RIAPGSAKVIDVYRTubiquitination[1]
365GFGVQELKILQSDLLubiquitination[1, 2, 3, 4, 5, 6]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [4] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [5] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [6] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302
Functional Description
 Specifically deaminates adenosine-37 to inosine in tRNA- Ala. 
Sequence Annotation
 DOMAIN 63 501 A to I editase.
 ACT_SITE 89 89 Proton donor (By similarity).
 METAL 87 87 Zinc (By similarity).
 METAL 142 142 Zinc (By similarity).
 METAL 299 299 Zinc (By similarity).
 BINDING 93 93 Inositol hexakisphosphate (By
 BINDING 94 94 Inositol hexakisphosphate (By
 BINDING 302 302 Inositol hexakisphosphate (By
 BINDING 305 305 Inositol hexakisphosphate (By
 BINDING 435 435 Inositol hexakisphosphate (By
 BINDING 470 470 Inositol hexakisphosphate (By  
Keyword
 Alternative splicing; Complete proteome; Hydrolase; Metal-binding; Polymorphism; Reference proteome; tRNA processing; Zinc. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 502 AA 
Protein Sequence
MWTADEIAQL CYEHYGIRLP KKGKPEPNHE WTLLAAVVKI QSPADKACDT PDKPVQVTKE 60
VVSMGTGTKC IGQSKMRKNG DILNDSHAEV IARRSFQRYL LHQLQLAATL KEDSIFVPGT 120
QKGVWKLRRD LIFVFFSSHT PCGDASIIPM LEFEDQPCCP VFRNWAHNSS VEASSNLEAP 180
GNERKCEDPD SPVTKKMRLE PGTAAREVTN GAAHHQSFGK QKSGPISPGI HSCDLTVEGL 240
ATVTRIAPGS AKVIDVYRTG AKCVPGEAGD SGKPGAAFHQ VGLLRVKPGR GDRTRSMSCS 300
DKMARWNVLG CQGALLMHLL EEPIYLSAVV IGKCPYSQEA MQRALIGRCQ NVSALPKGFG 360
VQELKILQSD LLFEQSRSAV QAKRADSPGR LVPCGAAISW SAVPEQPLDV TANGFPQGTT 420
KKTIGSLQAR SQISKVELFR SFQKLLSRIA RDKWPHSLRV QKLDTYQEYK EAASSYQEAW 480
STLRKQVFGS WIRNPPDYHQ FK 502 
Gene Ontology
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0003723; F:RNA binding; IMP:UniProtKB.
 GO:0008251; F:tRNA-specific adenosine deaminase activity; IMP:UniProtKB.
 GO:0008033; P:tRNA processing; IMP:UniProtKB. 
Interpro
 IPR002466; A_deamin. 
Pfam
 PF02137; A_deamin 
SMART
 SM00552; ADEAMc 
PROSITE
 PS50141; A_DEAMIN_EDITASE 
PRINTS