UniProt Accession

 TBB5_MOUSE; P99024; B1B178; P05218; Q3TFB6; Q3THH9; Q3TIL1; Q3UAV4; Q3UF52; Q8WUC1; Q9CY33 

Genbank Protein ID

 X04663; AK010960; AK011263; AK051164; AK051393; AK083327; AK146567; AK148978; AK151215; AK151670; AK160225; AK161575; AK165249; AK167691; AK167779; AK167808; AK167926; AK168271; AK169123; AK169210; AK169295; CR974451; BC003825; M28732 

Genbank Nucleotide ID

 CAA28369.1; BAB27292.1; BAB27504.1; BAC34541.1; BAC34623.1; BAC38866.1; BAE27265.1; BAE28709.1; BAE30210.1; BAE30596.1; BAE35700.1; BAE36471.1; BAE38103.1; BAE39738.1; BAE39811.1; BAE39835.1; BAE39931.1; BAE40217.1; BAE40903.1; BAE40982.1; BAE41051.1; CAO77890.1; AAH03825.1; AAA40510.1 

Protein Name

 Tubulin beta-5 chain 

Protein Synonyms/Alias

  

Gene Name

 Tubb5 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
58	YNEATGGKYVPRAIL	acetylation	[1, 2, 3]
58	YNEATGGKYVPRAIL	succinylation	[3]
58	YNEATGGKYVPRAIL	ubiquitination	[4]
297	TQQVFDAKNMMAACD	acetylation	[3]
297	TQQVFDAKNMMAACD	succinylation	[3]

Reference

 [1] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
 [2] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [3] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337]
 [4] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 

Functional Description

 Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. 

Sequence Annotation

 NP_BIND 140 146 GTP (Potential).
 MOD_RES 36 36 Phosphotyrosine (By similarity).
 MOD_RES 58 58 N6-acetyllysine; alternate (By
 MOD_RES 172 172 Phosphoserine; by CDK1 (By similarity).
 MOD_RES 318 318 Omega-N-methylarginine (By similarity).
 MOD_RES 340 340 Phosphotyrosine.
 MOD_RES 382 382 Phosphoserine.
 CROSSLNK 58 58 Glycyl lysine isopeptide (Lys-Gly)
 CROSSLNK 324 324 Glycyl lysine isopeptide (Lys-Gly)  

Keyword

 Acetylation; Complete proteome; Cytoplasm; Cytoskeleton; Direct protein sequencing; GTP-binding; Isopeptide bond; Methylation; Microtubule; Nucleotide-binding; Phosphoprotein; Reference proteome; Ubl conjugation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 444 AA 

Protein Sequence

Gene Ontology

 GO:0044297; C:cell body; IEA:Compara.
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0070062; C:extracellular vesicular exosome; IEA:Compara.
 GO:0005874; C:microtubule; IDA:MGI.
 GO:0005641; C:nuclear envelope lumen; IEA:Compara.
 GO:0005634; C:nucleus; IDA:MGI.
 GO:0005886; C:plasma membrane; IEA:Compara.
 GO:0045298; C:tubulin complex; NAS:UniProtKB.
 GO:0005525; F:GTP binding; NAS:UniProtKB.
 GO:0003924; F:GTPase activity; IEA:InterPro.
 GO:0005200; F:structural constituent of cytoskeleton; IDA:MGI.
 GO:0006457; P:protein folding; TAS:Reactome.
 GO:0051258; P:protein polymerization; IEA:InterPro.
 GO:0051225; P:spindle assembly; IDA:MGI. 

Interpro

 IPR013838; Beta-tubulin_BS.
 IPR002453; Beta_tubulin.
 IPR008280; Tub_FtsZ_C.
 IPR000217; Tubulin.
 IPR018316; Tubulin/FtsZ_2-layer-sand-dom.
 IPR023123; Tubulin_C.
 IPR017975; Tubulin_CS.
 IPR003008; Tubulin_FtsZ_GTPase. 

Pfam

 PF00091; Tubulin
 PF03953; Tubulin_C 

SMART

 SM00864; Tubulin
 SM00865; Tubulin_C 

PROSITE

 PS00227; TUBULIN
 PS00228; TUBULIN_B_AUTOREG 

PRINTS

 PR01163; BETATUBULIN.
 PR01161; TUBULIN.