UniProt Accession

 NUB1_HUMAN; Q9Y5A7; O95422; Q75MR9; Q8IX22; Q9BXR2 

Genbank Protein ID

 AF155099; AF300717; AF459743; AY129295; AC005486; BC046354; AF108083 

Genbank Nucleotide ID

 AAD42865.1; AAK21001.1; AAO14547.1; AAN01355.1; AAS02030.1; AAH46354.1; AAC82474.2 

Protein Name

 NEDD8 ultimate buster 1 

Protein Synonyms/Alias

 Negative regulator of ubiquitin-like proteins 1; Renal carcinoma antigen NY-REN-18 

Gene Name

 NUB1 

Gene Synonyms/Alias

 NYREN18 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
10	QKKYLQAKLTQFLRE	ubiquitination	[1]
167	QSEEDARKNFQLEEE	ubiquitination	[2]
315	NLAQKCFKNCYGENH	ubiquitination	[3]
371	NKARQLFKELYIDPS	ubiquitination	[1, 2, 3]

Reference

 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965] 

Functional Description

 Specific down-regulator of the NEDD8 conjugation system. Recruits NEDD8, UBD, and their conjugates to the proteasome for degradation. Isoform 1 promotes the degradation of NEDD8 more efficiently than isoform 2. 

Sequence Annotation

 DOMAIN 374 413 UBA 1.
 DOMAIN 424 470 UBA 2.
 DOMAIN 489 529 UBA 3.
 REGION 427 474 NEDD8-binding 1.
 REGION 550 598 NEDD8-binding 2.
 MOTIF 414 431 Nuclear localization signal (Probable).  

Keyword

 3D-structure; Alternative splicing; Coiled coil; Complete proteome; Nucleus; Polymorphism; Reference proteome; Repeat. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 615 AA 

Protein Sequence

Gene Ontology

 GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
 GO:0032436; P:positive regulation of proteasomal ubiquitin-dependent protein catabolic process; IMP:UniProtKB.
 GO:0016567; P:protein ubiquitination; IMP:UniProtKB.
 GO:0034341; P:response to interferon-gamma; IEP:UniProtKB.
 GO:0034612; P:response to tumor necrosis factor; IEP:UniProtKB.
 GO:0006511; P:ubiquitin-dependent protein catabolic process; IMP:UniProtKB. 

Interpro

 IPR009060; UBA-like.
 IPR000449; UBA/transl_elong_EF1B_N.
 IPR015940; UBA/transl_elong_EF1B_N_euk. 

Pfam

 PF00627; UBA 

SMART

 SM00165; UBA 

PROSITE

 PS50030; UBA 

PRINTS