UniProt Accession

 PDXJ_ECOLI; P0A794; P24223; Q2MAG6 

Genbank Protein ID

 M76470; M74526; D64044; U36841; U00096; AP009048 

Genbank Nucleotide ID

 AAA21845.1; AAA24315.1; AAA79826.1; AAC75617.1; BAE76740.1 

Protein Name

 Pyridoxine 5'-phosphate synthase 

Protein Synonyms/Alias

 PNP synthase 

Gene Name

 pdxJ 

Gene Synonyms/Alias

 b2564; JW2548 

Created Date

 July 27, 2013 

Organism

 Escherichia coli (strain K12) 

NCBI Taxa ID

 83333 

Lysine Modification

Position	Peptide	Type	References
97	FCCLVPEKRQEVTTE	acetylation	[1]

Reference

 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618] 

Functional Description

 Catalyzes the complicated ring closure reaction between the two acyclic compounds 1-deoxy-D-xylulose-5-phosphate (DXP) and 3-amino-2-oxopropyl phosphate (1-amino-acetone-3-phosphate or AAP) to form pyridoxine 5'-phosphate (PNP) and inorganic phosphate. 

Sequence Annotation

 REGION 11 12 1-deoxy-D-xylulose 5-phosphate binding.
 REGION 215 216 3-amino-2-oxopropyl phosphate binding.
 ACT_SITE 45 45 Proton acceptor.
 ACT_SITE 72 72 Proton acceptor.
 ACT_SITE 193 193 Proton donor.
 BINDING 9 9 3-amino-2-oxopropyl phosphate.
 BINDING 20 20 3-amino-2-oxopropyl phosphate.
 BINDING 47 47 1-deoxy-D-xylulose 5-phosphate.
 BINDING 52 52 1-deoxy-D-xylulose 5-phosphate.
 BINDING 102 102 1-deoxy-D-xylulose 5-phosphate.
 BINDING 194 194 3-amino-2-oxopropyl phosphate; via amide  

Keyword

 3D-structure; Complete proteome; Cytoplasm; Direct protein sequencing; Pyridoxine biosynthesis; Reference proteome; Transferase. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 243 AA 

Protein Sequence

Gene Ontology

 GO:0005829; C:cytosol; IDA:UniProtKB.
 GO:0033856; F:pyridoxine 5'-phosphate synthase activity; IDA:EcoCyc.
 GO:0008615; P:pyridoxine biosynthetic process; IMP:EcoliWiki. 

Interpro

 IPR013785; Aldolase_TIM.
 IPR004569; PyrdxlP_synth_PdxJ. 

Pfam

 PF03740; PdxJ 

SMART

  

PROSITE

  

PRINTS