CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-016969
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Phospholipase D3 
Protein Synonyms/Alias
 PLD 3; Choline phosphatase 3; HindIII K4L homolog; Hu-K4; Phosphatidylcholine-hydrolyzing phospholipase D3 
Gene Name
 PLD3 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
4****MKPKLMYQELKubiquitination[1]
11KLMYQELKVPAEEPAubiquitination[1, 2, 3, 4, 5]
30MNEIEAWKAAEKKARubiquitination[1, 4]
157QLQTLAPKGVNVRIAubiquitination[2, 5, 6]
167NVRIAVSKPSGPQPQubiquitination[1, 4]
309SGRTPDLKALLNVVDubiquitination[1, 2, 3, 4, 5, 6]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
 Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
 J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572]
 [4] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [5] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [6] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961
Functional Description
  
Sequence Annotation
 DOMAIN 196 223 PLD phosphodiesterase 1.
 DOMAIN 411 437 PLD phosphodiesterase 2.
 ACT_SITE 201 201 Potential.
 ACT_SITE 203 203 Potential.
 ACT_SITE 208 208 Potential.
 CARBOHYD 97 97 N-linked (GlcNAc...).
 CARBOHYD 132 132 N-linked (GlcNAc...).  
Keyword
 Complete proteome; Endoplasmic reticulum; Glycoprotein; Hydrolase; Lipid degradation; Lipid metabolism; Membrane; Reference proteome; Repeat; Signal-anchor; Transmembrane; Transmembrane helix. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 490 AA 
Protein Sequence
MKPKLMYQEL KVPAEEPANE LPMNEIEAWK AAEKKARWVL LVLILAVVGF GALMTQLFLW 60
EYGDLHLFGP NQRPAPCYDP CEAVLVESIP EGLDFPNAST GNPSTSQAWL GLLAGAHSSL 120
DIASFYWTLT NNDTHTQEPS AQQGEEVLRQ LQTLAPKGVN VRIAVSKPSG PQPQADLQAL 180
LQSGAQVRMV DMQKLTHGVL HTKFWVVDQT HFYLGSANMD WRSLTQVKEL GVVMYNCSCL 240
ARDLTKIFEA YWFLGQAGSS IPSTWPRFYD TRYNQETPME ICLNGTPALA YLASAPPPLC 300
PSGRTPDLKA LLNVVDNARS FIYVAVMNYL PTLEFSHPHR FWPAIDDGLR RATYERGVKV 360
RLLISCWGHS EPSMRAFLLS LAALRDNHTH SDIQVKLFVV PADEAQARIP YARVNHNKYM 420
VTERATYIGT SNWSGNYFTE TAGTSLLVTQ NGRGGLRSQL EAIFLRDWDS PYSHDLDTSA 480
DSVGNACRLL 490 
Gene Ontology
 GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
 GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
 GO:0070290; F:NAPE-specific phospholipase D activity; IEA:EC.
 GO:0004630; F:phospholipase D activity; TAS:ProtInc.
 GO:0016042; P:lipid catabolic process; IEA:UniProtKB-KW.
 GO:0006655; P:phosphatidylglycerol biosynthetic process; TAS:Reactome.
 GO:0044281; P:small molecule metabolic process; TAS:Reactome. 
Interpro
 IPR001736; PLipase_D/transphosphatidylase. 
Pfam
  
SMART
 SM00155; PLDc 
PROSITE
 PS50035; PLD 
PRINTS