CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-022851
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Glyoxylate reductase/hydroxypyruvate reductase 
Protein Synonyms/Alias
  
Gene Name
 GRHPR 
Gene Synonyms/Alias
 GLXR; MSTP035 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
173QAIARRLKPFGVQRFubiquitination[1]
235KDFFQKMKETAVFINubiquitination[1]
262YQALASGKIAAAGLDubiquitination[2, 3, 4]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [4] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661] 
Functional Description
 Enzyme with hydroxy-pyruvate reductase, glyoxylate reductase and D-glycerate dehydrogenase enzymatic activities. Reduces hydroxypyruvate to D-glycerate, glyoxylate to glycolate oxidizes D-glycerate to hydroxypyruvate. 
Sequence Annotation
 NP_BIND 162 164 NADP.
 NP_BIND 185 188 NADP.
 REGION 83 84 Substrate binding.
 REGION 293 296 Substrate binding.
 ACT_SITE 293 293 Proton donor.
 BINDING 217 217 NADP.
 BINDING 243 243 NADP; via carbonyl oxygen.
 BINDING 245 245 Substrate.
 BINDING 269 269 Substrate.
 BINDING 295 295 NADP; via amide nitrogen.  
Keyword
 3D-structure; Complete proteome; NADP; Oxidoreductase; Polymorphism; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 328 AA 
Protein Sequence
MRPVRLMKVF VTRRIPAEGR VALARAADCE VEQWDSDEPI PAKELERGVA GAHGLLCLLS 60
DHVDKRILDA AGANLKVIST MSVGIDHLAL DEIKKRGIRV GYTPDVLTDT TAELAVSLLL 120
TTCRRLPEAI EEVKNGGWTS WKPLWLCGYG LTQSTVGIIG LGRIGQAIAR RLKPFGVQRF 180
LYTGRQPRPE EAAEFQAEFV STPELAAQSD FIVVACSLTP ATEGLCNKDF FQKMKETAVF 240
INISRGDVVN QDDLYQALAS GKIAAAGLDV TSPEPLPTNH PLLTLKNCVI LPHIGSATHR 300
TRNTMSLLAA NNLLAGLRGE PMPSELKL 328 
Gene Ontology
 GO:0005782; C:peroxisomal matrix; TAS:Reactome.
 GO:0008465; F:glycerate dehydrogenase activity; IDA:UniProtKB.
 GO:0030267; F:glyoxylate reductase (NADP) activity; IDA:UniProtKB.
 GO:0016618; F:hydroxypyruvate reductase activity; IDA:UniProtKB.
 GO:0051287; F:NAD binding; TAS:UniProtKB.
 GO:0070402; F:NADPH binding; IDA:UniProtKB.
 GO:0042803; F:protein homodimerization activity; IDA:UniProtKB.
 GO:0034641; P:cellular nitrogen compound metabolic process; TAS:Reactome.
 GO:0007588; P:excretion; IMP:UniProtKB.
 GO:0046487; P:glyoxylate metabolic process; TAS:Reactome.
 GO:0051259; P:protein oligomerization; IDA:UniProtKB. 
Interpro
 IPR006139; D-isomer_2_OHA_DH_cat_dom.
 IPR006140; D-isomer_2_OHA_DH_NAD-bd.
 IPR016040; NAD(P)-bd_dom. 
Pfam
 PF00389; 2-Hacid_dh
 PF02826; 2-Hacid_dh_C 
SMART
  
PROSITE
 PS00065; D_2_HYDROXYACID_DH_1
 PS00670; D_2_HYDROXYACID_DH_2
 PS00671; D_2_HYDROXYACID_DH_3 
PRINTS