UniProt Accession

 P53_MOUSE; P02340; Q9QUP3 

Genbank Protein ID

 X00876; X00877; X00878; X00879; X00880; X00881; X00882; X00883; X00884; X00885; X01237; X00741; M13872; M13873; M13874; AB021961; AF151353; AB017815; AB017816; AB020317; BC005448; S77930 

Genbank Nucleotide ID

 CAA25420.1; CAA25420.1; CAA25420.1; CAA25420.1; CAA25420.1; CAA25420.1; CAA25420.1; CAA25420.1; CAA25420.1; CAA25420.1; CAA25625.1; CAA25323.1; AAA39881.1; AAA39882.1; AAA39883.1; BAA82344.1; AAD39535.1; BAA82339.1; BAA82340.1; BAA82343.1; AAH05448.1; AAB21108.2 

Protein Name

 Cellular tumor antigen p53 

Protein Synonyms/Alias

 Tumor suppressor p53 

Gene Name

 Tp53 

Gene Synonyms/Alias

 P53; Trp53 

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
314	SASPPQKKKPLDGEY	acetylation	[1]
364	RAHSSYLKTKKGQST	acetylation	[2, 3]
366	HSSYLKTKKGQSTSR	acetylation	[2]
367	SSYLKTKKGQSTSRH	acetylation	[2]
375	GQSTSRHKKTMVKKV	acetylation	[2, 3]
376	QSTSRHKKTMVKKVG	acetylation	[2, 3]
380	RHKKTMVKKVGPDSD	acetylation	[2]

Reference

 [1] Acetylation of mouse p53 at lysine 317 negatively regulates p53 apoptotic activities after DNA damage.
 Chao C, Wu Z, Mazur SJ, Borges H, Rossi M, Lin T, Wang JY, Anderson CW, Appella E, Xu Y.
 Mol Cell Biol. 2006 Sep;26(18):6859-69. [PMID: 16943427]
 [2] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
 [3] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337] 

Functional Description

 Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression. In cooperation with mitochondrial PPIF is involved in activating oxidative stress-induced necrosis; the function is largely independent of transcription. Prevents CDK7 kinase activity when associated to CAK complex in response to DNA damage, thus stopping cell cycle progression (By similarity). Induces the transcription of long intergenic non-coding RNA p21 (lincRNA-p21) and lincRNA-Mkln1. LincRNA-p21 participates in TP53-dependent transcriptional repression leading to apoptosis, but seems to have to effect on cell-cycle regulation. 

Sequence Annotation

 DNA_BIND 96 286 By similarity.
 REGION 1 42 Transcription activation (acidic).
 REGION 60 104 Interaction with WWOX (By similarity).
 REGION 94 364 Interaction with HIPK1.
 REGION 94 294 Required for interaction with ZNF385A (By
 REGION 107 230 Required for interaction with FBXO42 (By
 REGION 110 286 Interaction with AXIN1.
 REGION 253 291 Interaction with E4F1 (By similarity).
 REGION 267 274 Interaction with DNA.
 REGION 313 354 Interaction with HIPK2 (By similarity).
 REGION 319 350 Oligomerization.
 REGION 353 357 Interaction with USP7 (By similarity).
 REGION 362 381 Basic (repression of DNA-binding).
 MOTIF 299 315 Bipartite nuclear localization signal (By
 MOTIF 333 344 Nuclear export signal (By similarity).
 MOTIF 364 366 [KR]-[STA]-K motif.
 METAL 170 170 Zinc.
 METAL 173 173 Zinc.
 METAL 232 232 Zinc.
 METAL 236 236 Zinc.
 MOD_RES 9 9 Phosphoserine; by HIPK4.
 MOD_RES 15 15 Phosphoserine; by CDK5, PRPK, AMPK, NUAK1
 MOD_RES 18 18 Phosphothreonine; by CK1, VRK1 and VRK2
 MOD_RES 20 20 Phosphoserine; by CHEK2, CK1 and PLK3 (By
 MOD_RES 34 34 Phosphoserine; by MAPKAPK5.
 MOD_RES 114 114 N6-acetyllysine; by KAT6A (By
 MOD_RES 177 177 Phosphoserine; by AURKB (By similarity).
 MOD_RES 263 263 Phosphoserine; by AURKB (By similarity).
 MOD_RES 278 278 Phosphothreonine; by AURKB (By
 MOD_RES 299 299 N6-acetyllysine (By similarity).
 MOD_RES 309 309 Phosphoserine; by AURKA, CDK1 and CDK2
 MOD_RES 364 364 N6,N6-dimethyllysine; alternate (By
 MOD_RES 364 364 N6-methyllysine; by SMYD2; alternate (By
 MOD_RES 366 366 N6-methyllysine; by SETD7 (By
 MOD_RES 367 367 N6,N6-dimethyllysine; by EHMT1 and EHMT2;
 MOD_RES 367 367 N6-acetyllysine; alternate (By
 MOD_RES 375 375 N6-acetyllysine (By similarity).
 MOD_RES 376 376 N6,N6-dimethyllysine; alternate (By
 MOD_RES 376 376 N6-acetyllysine; by KAT6A; alternate (By
 MOD_RES 376 376 N6-methyllysine; by SETD8; alternate (By
 MOD_RES 386 386 Phosphoserine; by CK2, CDK2 and NUAK1 (By
 CROSSLNK 285 285 Glycyl lysine isopeptide (Lys-Gly)
 CROSSLNK 286 286 Glycyl lysine isopeptide (Lys-Gly)
 CROSSLNK 380 380 Glycyl lysine isopeptide (Lys-Gly)  

Keyword

 3D-structure; Acetylation; Activator; Apoptosis; Cell cycle; Complete proteome; Cytoplasm; Disease mutation; DNA-binding; Endoplasmic reticulum; Isopeptide bond; Metal-binding; Methylation; Mitochondrion; Necrosis; Nucleus; Phosphoprotein; Reference proteome; Transcription; Transcription regulation; Tumor suppressor; Ubl conjugation; Zinc. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 387 AA 

Protein Sequence

Gene Ontology

 GO:0000785; C:chromatin; IBA:RefGenome.
 GO:0005829; C:cytosol; IDA:MGI.
 GO:0005783; C:endoplasmic reticulum; IEA:UniProtKB-SubCell.
 GO:0005759; C:mitochondrial matrix; IEA:UniProtKB-SubCell.
 GO:0005739; C:mitochondrion; IDA:UniProtKB.
 GO:0005730; C:nucleolus; ISS:UniProtKB.
 GO:0005657; C:replication fork; IDA:MGI.
 GO:0005667; C:transcription factor complex; IBA:RefGenome.
 GO:0005524; F:ATP binding; ISS:UniProtKB.
 GO:0003682; F:chromatin binding; IDA:MGI.
 GO:0005507; F:copper ion binding; ISS:UniProtKB.
 GO:0003684; F:damaged DNA binding; IBA:RefGenome.
 GO:0003690; F:double-stranded DNA binding; IBA:RefGenome.
 GO:0035033; F:histone deacetylase regulator activity; IDA:MGI.
 GO:0002039; F:p53 binding; IBA:RefGenome.
 GO:0001077; F:RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription; IDA:MGI.
 GO:0043565; F:sequence-specific DNA binding; IBA:RefGenome.
 GO:0044212; F:transcription regulatory region DNA binding; IEA:InterPro.
 GO:0002326; P:B cell lineage commitment; IMP:MGI.
 GO:0007569; P:cell aging; ISS:UniProtKB.
 GO:0071479; P:cellular response to ionizing radiation; IGI:MGI.
 GO:0034644; P:cellular response to UV; IGI:MGI.
 GO:0007417; P:central nervous system development; IGI:MGI.
 GO:0051276; P:chromosome organization; IGI:MGI.
 GO:0008340; P:determination of adult lifespan; IMP:BHF-UCL.
 GO:0006977; P:DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; IGI:MGI.
 GO:0006978; P:DNA damage response, signal transduction by p53 class mediator resulting in transcription of p21 class mediator; IBA:RefGenome.
 GO:0000733; P:DNA strand renaturation; ISS:UniProtKB.
 GO:0006302; P:double-strand break repair; IMP:MGI.
 GO:0048568; P:embryonic organ development; IGI:MGI.
 GO:0007369; P:gastrulation; IGI:MGI.
 GO:0001701; P:in utero embryonic development; IGI:MGI.
 GO:0006917; P:induction of apoptosis; IDA:MGI.
 GO:0042771; P:intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator; IMP:BHF-UCL.
 GO:0071850; P:mitotic cell cycle arrest; IDA:MGI.
 GO:0035264; P:multicellular organism growth; IGI:MGI.
 GO:0043066; P:negative regulation of apoptotic process; IMP:MGI.
 GO:0030308; P:negative regulation of cell growth; ISS:UniProtKB.
 GO:0008156; P:negative regulation of DNA replication; IDA:MGI.
 GO:0048147; P:negative regulation of fibroblast proliferation; IMP:MGI.
 GO:1901525; P:negative regulation of macromitophagy; IMP:MGI.
 GO:0007406; P:negative regulation of neuroblast proliferation; IGI:MGI.
 GO:2000378; P:negative regulation of reactive oxygen species metabolic process; IMP:MGI.
 GO:0000122; P:negative regulation of transcription from RNA polymerase II promoter; IMP:BHF-UCL.
 GO:0030512; P:negative regulation of transforming growth factor beta receptor signaling pathway; IMP:MGI.
 GO:0051402; P:neuron apoptotic process; IMP:MGI.
 GO:0006289; P:nucleotide-excision repair; ISS:UniProtKB.
 GO:0097252; P:oligodendrocyte apoptotic process; ISS:UniProtKB.
 GO:0010666; P:positive regulation of cardiac muscle cell apoptotic process; IMP:MGI.
 GO:0090343; P:positive regulation of cell aging; IMP:BHF-UCL.
 GO:0031065; P:positive regulation of histone deacetylation; IDA:MGI.
 GO:0043525; P:positive regulation of neuron apoptotic process; IDA:MGI.
 GO:0050731; P:positive regulation of peptidyl-tyrosine phosphorylation; IMP:BHF-UCL.
 GO:0090200; P:positive regulation of release of cytochrome c from mitochondria; ISS:UniProtKB.
 GO:0070245; P:positive regulation of thymocyte apoptotic process; IMP:BHF-UCL.
 GO:0000060; P:protein import into nucleus, translocation; IDA:MGI.
 GO:0051262; P:protein tetramerization; IEA:InterPro.
 GO:1902108; P:regulation of mitochondrial membrane permeability involved in apoptotic process; IGI:MGI.
 GO:0034103; P:regulation of tissue remodeling; IMP:MGI.
 GO:0001836; P:release of cytochrome c from mitochondria; IDA:MGI.
 GO:0042493; P:response to drug; IDA:MGI.
 GO:0010332; P:response to gamma radiation; IDA:MGI.
 GO:0002931; P:response to ischemia; IMP:MGI.
 GO:0006979; P:response to oxidative stress; IMP:MGI.
 GO:0009651; P:response to salt stress; IGI:MGI.
 GO:0010165; P:response to X-ray; IDA:MGI.
 GO:0009303; P:rRNA transcription; IGI:MGI.
 GO:0001756; P:somitogenesis; IGI:MGI.
 GO:0033077; P:T cell differentiation in thymus; IGI:MGI.
 GO:0002360; P:T cell lineage commitment; IMP:MGI.
 GO:0002309; P:T cell proliferation involved in immune response; IGI:MGI.
 GO:0007179; P:transforming growth factor beta receptor signaling pathway; IGI:MGI. 

Interpro

 IPR008967; p53-like_TF_DNA-bd.
 IPR012346; p53/RUNT-type_TF_DNA-bd.
 IPR011615; p53_DNA-bd.
 IPR010991; p53_tetrameristn.
 IPR013872; p53_transactivation_domain.
 IPR002117; p53_tumour_suppressor. 

Pfam

 PF00870; P53
 PF08563; P53_TAD
 PF07710; P53_tetramer 

SMART

  

PROSITE

 PS00348; P53 

PRINTS

 PR00386; P53SUPPRESSR.