CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-037251
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
  
Protein Name
 5'-nucleotidase domain-containing protein 2 
Protein Synonyms/Alias
  
Gene Name
 NT5DC2 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
139DILIEHYKYPEGIRKubiquitination[1, 2, 3, 4]
146KYPEGIRKYDYNPSFacetylation[5]
146KYPEGIRKYDYNPSFubiquitination[2]
354PFRKLDEKGSLQWDRubiquitination[2]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [4] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [5] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377
Functional Description
  
Sequence Annotation
 ACT_SITE 110 110 Nucleophile (By similarity).
 ACT_SITE 112 112 Proton donor (By similarity).
 METAL 110 110 Magnesium (By similarity).
 METAL 112 112 Magnesium; via carbonyl oxygen (By
 METAL 395 395 Magnesium (By similarity).  
Keyword
 Complete proteome; Magnesium; Metal-binding; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 557 AA 
Protein Sequence
MAGAGLRAAA RRWLLCGGHG GPRAASSSPS CPGCGPPGPG AHCPGVPRSA PAQAPTSGAD 60
LSAHLWARYQ DMRRLVHDLL PPEVCSLLNP AAIYANNEIS LRDVEVYGFD YDYTLAQYAD 120
ALHPEIFSTA RDILIEHYKY PEGIRKYDYN PSFAIRGLHY DIQKSLLMKI DAFHYVQLGT 180
AYRGLQPVPD EEVIELYGGT QHIPLYQMSG FYGKGPSIKQ FMDIFSLPEM ALLSCVVDYF 240
LGHSLEFDQA HLYKDVTDAI RDVHVKGLMY QWIEQDMEKY ILRGDETFAV LSRLVAHGKQ 300
LFLITNSPFS FVDKGMRHMV GPDWRQLFDV VIVQADKPSF FTDRRKPFRK LDEKGSLQWD 360
RITRLEKGKI YRQGNLFDFL RLTEWRGPRV LYFGDHLYSD LADLMLRHGW RTGAIIPELE 420
REIRIINTEQ YMHSLTWQQA LTGLLERMQT YQDAESRQVL AAWMKERQEL RCITKALFNA 480
QFGSIFRTFH NPTYFSRRLV RFSDLYMASL SCLLNYRVDF TFYPRRTPLQ HEAPLWMDQL 540
CTGCMKTPFL GDMAHIR 557 
Gene Ontology
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW. 
Interpro
 IPR023214; HAD-like_dom.
 IPR008380; HAD-SF_hydro_IG_5-nucl.
 IPR016695; Pur_nucleotidase. 
Pfam
 PF05761; 5_nucleotid 
SMART
  
PROSITE
  
PRINTS