CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-018855
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 DNA repair protein RAD50 
Protein Synonyms/Alias
 hRAD50 
Gene Name
 RAD50 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
68GDFPPGTKGNTFVHDubiquitination[1]
181EGKALKQKFDEIFSAubiquitination[1]
193FSATRYIKALETLRQubiquitination[1]
217KEYQMELKYLKQYKEubiquitination[1]
301ELEEKMEKVFQGTDEubiquitination[1]
467QNELKNVKYELQQLEubiquitination[1]
851KLDTVSSKIELNRKLubiquitination[1]
1125RTTELVNKDLDIYYKubiquitination[1]
1140TLDQAIMKFHSMKMEubiquitination[1]
1291GRSEYVEKFYRIKKNubiquitination[1]
Reference
 [1] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 
Functional Description
 Component of the MRN complex, which plays a central role in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity and meiosis. The complex possesses single-strand endonuclease activity and double-strand- specific 3'-5' exonuclease activity, which are provided by MRE11A. RAD50 may be required to bind DNA ends and hold them in close proximity. This could facilitate searches for short or long regions of sequence homology in the recombining DNA templates, and may also stimulate the activity of DNA ligases and/or restrict the nuclease activity of MRE11A to prevent nucleolytic degradation past a given point. The complex may also be required for DNA damage signaling via activation of the ATM kinase. In telomeres the MRN complex may modulate t-loop formation. 
Sequence Annotation
 DOMAIN 635 734 Zinc-hook.
 NP_BIND 36 43 ATP (Potential).
 METAL 681 681 Zinc (By similarity).
 METAL 684 684 Zinc (By similarity).
 MOD_RES 237 237 Phosphoserine (By similarity).
 MOD_RES 635 635 Phosphoserine.
 MOD_RES 690 690 Phosphothreonine.
 MOD_RES 959 959 N6-acetyllysine.  
Keyword
 Acetylation; Alternative splicing; ATP-binding; Cell cycle; Chromosome; Coiled coil; Complete proteome; DNA damage; DNA repair; Hydrolase; Meiosis; Metal-binding; Nucleotide-binding; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Telomere; Zinc. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1312 AA 
Protein Sequence
MLIFSVRDMF AKMSILGVRS FGIEDKDKQI ITFFSPLTIL VGPNGAGKTT IIECLKYICT 60
GDFPPGTKGN TFVHDPKVAQ ETDVRAQIRL QFRDVNGELI AVQRSMVCTQ KSKKTEFKTL 120
EGVITRTKHG EKVSLSSKCA EIDREMISSL GVSKAVLNNV IFCHQEDSNW PLSEGKALKQ 180
KFDEIFSATR YIKALETLRQ VRQTQGQKVK EYQMELKYLK QYKEKACEIR DQITSKEAQL 240
TSSKEIVKSY ENELDPLKNR LKEIEHNLSK IMKLDNEIKA LDSRKKQMEK DNSELEEKME 300
KVFQGTDEQL NDLYHNHQRT VREKERKLVD CHRELEKLNK ESRLLNQEKS ELLVEQGRLQ 360
LQADRHQEHI RARDSLIQSL ATQLELDGFE RGPFSERQIK NFHKLVRERQ EGEAKTANQL 420
MNDFAEKETL KQKQIDEIRD KKTGLGRIIE LKSEILSKKQ NELKNVKYEL QQLEGSSDRI 480
LELDQELIKA ERELSKAEKN SNVETLKMEV ISLQNEKADL DRTLRKLDQE MEQLNHHTTT 540
RTQMEMLTKD KADKDEQIRK IKSRHSDELT SLLGYFPNKK QLEDWLHSKS KEINQTRDRL 600
AKLNKELASS EQNKNHINNE LKRKEEQLSS YEDKLFDVCG SQDFESDLDR LKEEIEKSSK 660
QRAMLAGATA VYSQFITQLT DENQSCCPVC QRVFQTEAEL QEVISDLQSK LRLAPDKLKS 720
TESELKKKEK RRDEMLGLVP MRQSIIDLKE KEIPELRNKL QNVNRDIQRL KNDIEEQETL 780
LGTIMPEEES AKVCLTDVTI MERFQMELKD VERKIAQQAA KLQGIDLDRT VQQVNQEKQE 840
KQHKLDTVSS KIELNRKLIQ DQQEQIQHLK STTNELKSEK LQISTNLQRR QQLEEQTVEL 900
STEVQSLYRE IKDAKEQVSP LETTLEKFQQ EKEELINKKN TSNKIAQDKL NDIKEKVKNI 960
HGYMKDIENY IQDGKDDYKK QKETELNKVI AQLSECEKHK EKINEDMRLM RQDIDTQKIQ 1020
ERWLQDNLTL RKRNEELKEV EEERKQHLKE MGQMQVLQMK SEHQKLEENI DNIKRNHNLA 1080
LGRQKGYEEE IIHFKKELRE PQFRDAEEKY REMMIVMRTT ELVNKDLDIY YKTLDQAIMK 1140
FHSMKMEEIN KIIRDLWRST YRGQDIEYIE IRSDADENVS ASDKRRNYNY RVVMLKGDTA 1200
LDMRGRCSAG QKVLASLIIR LALAETFCLN CGIIALDEPT TNLDRENIES LAHALVEIIK 1260
SRSQQRNFQL LVITHDEDFV ELLGRSEYVE KFYRIKKNID QCSEIVKCSV SSLGFNVH 1318 
Gene Ontology
 GO:0030870; C:Mre11 complex; IDA:UniProtKB.
 GO:0000784; C:nuclear chromosome, telomeric region; IDA:BHF-UCL.
 GO:0005654; C:nucleoplasm; TAS:Reactome.
 GO:0045120; C:pronucleus; IEA:Compara.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0003677; F:DNA binding; IDA:BHF-UCL.
 GO:0004518; F:nuclease activity; IEA:InterPro.
 GO:0030674; F:protein binding, bridging; IDA:UniProtKB.
 GO:0008270; F:zinc ion binding; IEA:InterPro.
 GO:0032508; P:DNA duplex unwinding; IMP:BHF-UCL.
 GO:0000724; P:double-strand break repair via homologous recombination; TAS:Reactome.
 GO:0090305; P:nucleic acid phosphodiester bond hydrolysis; IEA:GOC.
 GO:0033674; P:positive regulation of kinase activity; IDA:BHF-UCL.
 GO:0031954; P:positive regulation of protein autophosphorylation; IDA:BHF-UCL.
 GO:0007131; P:reciprocal meiotic recombination; TAS:ProtInc.
 GO:0000019; P:regulation of mitotic recombination; IDA:UniProtKB.
 GO:0007004; P:telomere maintenance via telomerase; IDA:UniProtKB. 
Interpro
 IPR027417; P-loop_NTPase.
 IPR004584; Rad50.
 IPR007517; Rad50_Zn_hook.
 IPR013134; Zn_hook_Rad50. 
Pfam
 PF04423; Rad50_zn_hook 
SMART
  
PROSITE
 PS51131; ZN_HOOK 
PRINTS