CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-015949
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Wings apart-like protein homolog 
Protein Synonyms/Alias
 Friend of EBNA2 protein 
Gene Name
 WAPAL 
Gene Synonyms/Alias
 FOE; KIAA0261; WAPL 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
848SAKLLNEKDMNKIKEubiquitination[1]
1263AVGTTGQKSISRVIEubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 
Functional Description
 Regulator of sister chromatid cohesion in mitosis which negatively regulates cohesin association with chromatin. Involved in both sister chromatid cohesion during interphase and sister- chromatid resolution during early stages of mitosis. Couples DNA replication to sister chromatid cohesion. Cohesion ensures that chromosome partitioning is accurate in both meiotic and mitotic cells and plays an important role in DNA repair. 
Sequence Annotation
 DOMAIN 626 1169 WAPL.
 REGION 1 659 Mediates interaction with the cohesin
 MOTIF 73 75 FGF motif 1.
 MOTIF 429 431 FGF motif 2.
 MOTIF 453 455 FGF motif 3.
 MOD_RES 77 77 Phosphoserine.
 MOD_RES 168 168 N6-acetyllysine.
 MOD_RES 221 221 Phosphoserine.
 MOD_RES 223 223 Phosphoserine.
 MOD_RES 226 226 Phosphoserine.
 MOD_RES 380 380 Phosphoserine.
 MOD_RES 459 459 Phosphoserine.
 MOD_RES 461 461 Phosphoserine.
 MOD_RES 904 904 Phosphoserine.  
Keyword
 Acetylation; Alternative splicing; Cell cycle; Cell division; Chromosome; Coiled coil; Complete proteome; Cytoplasm; Host-virus interaction; Mitosis; Nucleus; Phosphoprotein; Polymorphism; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1190 AA 
Protein Sequence
MVQGPVQTPA LTIHRRKRRR SCPPNRASYL PKAESLASLG SHLPALLSRA RVPRPPAGRR 60
ERERRRRPVA KAPARLRGEY ETGVKMTSRF GKTYSRKGGN GSSKFDEVFS NKRTTLSTKW 120
GETTFMAKLG QKRPNFKPDI QEIPKKPKVE EESTGDPFGF DSDDESLPVS SKNLAQVKCS 180
SYSESSEAAQ LEEVTSVLEA NSKISHVVVE DTVVSDKCFP LEDTLLGKEK STNRIVEDDA 240
SISSCNKLIT SDKVENFHEE HEKNSHHIHK NADDSTKKPN AETTVASEIK ETNDTWNSQF 300
GKRPESPSEI SPIKGSVRTG LFEWDNDFED IRSEDCILSL DSDPLLEMKD DDFKNRLENL 360
NEAIEEDIVQ SVLRPTNCRT YCRANKTKSS QGASNFDKLM DGTSQALAKA NSESSKDGLN 420
QAKKGGVSCG TSFRGTVGRT RDYTVLHPSC LSVCNVTIQD TMERSMDEFT ASTPADLGEA 480
GRLRKKADIA TSKTTTRFRP SNTKSKKDVK LEFFGFEDHE TGGDEGGSGS SNYKIKYFGF 540
DDLSESEDDE DDDCQVERKT SKKRTKTAPS PSLQPPPESN DNSQDSQSGT NNAENLDFTE 600
DLPGVPESVK KPINKQGDKS KENTRKIFSG PKRSPTKAVY NARHWNHPDS EELPGPPVVK 660
PQSVTVRLSS KEPNQKDDGV FKAPAPPSKV IKTVTIPTQP YQDIVTALKC RREDKELYTV 720
VQHVKHFNDV VEFGENQEFT DDIEYLLSGL KSTQPLNTRC LSVISLATKC AMPSFRMHLR 780
AHGMVAMVFK TLDDSQHHQN LSLCTAALMY ILSRDRLNMD LDRASLDLMI RLLELEQDAS 840
SAKLLNEKDM NKIKEKIRRL CETVHNKHLD LENITTGHLA METLLSLTSK RAGDWFKEEL 900
RLLGGLDHIV DKVKECVDHL SRDEDEEKLV ASLWGAERCL RVLESVTVHN PENQSYLIAY 960
KDSQLIVSSA KALQHCEELI QQYNRAEDSI CLADSKPLPH QNVTNHVGKA VEDCMRAIIG 1020
VLLNLTNDNE WGSTKTGEQD GLIGTALNCV LQVPKYLPQE QRFDIRVLGL GLLINLVEYS 1080
ARNRHCLVNM ETSCSFDSSI CSGEGDDSLR IGGQVHAVQA LVQLFLERER AAQLAESKTD 1140
ELIKDAPTTQ HDKSGEWQET SGEIQWVSTE KTDGTEEKHK KEEEDEELDL NKALQHAGKH 1200
MEDCIVASYT ALLLGCLCQE SPINVTTVRE YLPEGDFSIM TEMLKKFLSF MNLTCAVGTT 1260
GQKSISRVIE YLEHC 1275 
Gene Ontology
 GO:0000785; C:chromatin; IDA:UniProtKB.
 GO:0000775; C:chromosome, centromeric region; TAS:Reactome.
 GO:0008278; C:cohesin complex; IDA:UniProtKB.
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0015630; C:microtubule cytoskeleton; IDA:HPA.
 GO:0005654; C:nucleoplasm; TAS:Reactome.
 GO:0000795; C:synaptonemal complex; IEA:Compara.
 GO:0000910; P:cytokinesis; TAS:Reactome.
 GO:0007067; P:mitosis; IEA:UniProtKB-KW.
 GO:0000278; P:mitotic cell cycle; TAS:Reactome.
 GO:0035562; P:negative regulation of chromatin binding; IMP:UniProtKB.
 GO:0008156; P:negative regulation of DNA replication; IMP:UniProtKB.
 GO:0045875; P:negative regulation of sister chromatid cohesion; IMP:UniProtKB.
 GO:0071168; P:protein localization to chromatin; IMP:UniProtKB.
 GO:0071922; P:regulation of cohesin localization to chromatin; IMP:UniProtKB.
 GO:0009636; P:response to toxic substance; IEA:Compara.
 GO:0019048; P:virus-host interaction; IEA:UniProtKB-KW. 
Interpro
 IPR016024; ARM-type_fold.
 IPR012502; WAPL_metazoan_plants.
 IPR022771; WAPL_prot. 
Pfam
 PF07814; WAPL 
SMART
  
PROSITE
 PS51271; WAPL 
PRINTS