CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-000984
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Leupaxin 
Protein Synonyms/Alias
  
Gene Name
 LPXN 
Gene Synonyms/Alias
 LDLP 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
86KESPPPSKTSAAAQLubiquitination[1]
106HLTEMQAKVAVRADAubiquitination[1]
157GHCASCQKPIAGKVIubiquitination[2]
260DKKPYCRKDFLAMFSubiquitination[1, 3]
364FCLTQLSKGIFREQNubiquitination[1]
373IFREQNDKTYCQPCFubiquitination[4]
382YCQPCFNKLFPL***ubiquitination[4]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [4] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724] 
Functional Description
 Transcriptional coactivator for androgen receptor (AR) and serum response factor (SRF). Contributes to the regulation of cell adhesion, spreading and cell migration and acts as a negative regulator in integrin-mediated cell adhesion events. Suppresses the integrin-induced tyrosine phosphorylation of paxillin (PXN). May play a critical role as an adapter protein in the formation of the adhesion zone in osteoclasts. Negatively regulates B-cell antigen receptor (BCR) signaling. 
Sequence Annotation
 DOMAIN 150 208 LIM zinc-binding 1.
 DOMAIN 209 267 LIM zinc-binding 2.
 DOMAIN 268 326 LIM zinc-binding 3.
 DOMAIN 327 386 LIM zinc-binding 4.
 MOTIF 3 15 LD motif 1.
 MOTIF 70 82 LD motif 2.
 MOTIF 92 103 LD motif 3.
 MOD_RES 19 19 Phosphoserine.
 MOD_RES 22 22 Phosphotyrosine.
 MOD_RES 62 62 Phosphotyrosine.
 MOD_RES 72 72 Phosphotyrosine; by LYN.  
Keyword
 3D-structure; Activator; Alternative splicing; Cell adhesion; Cell junction; Cell membrane; Cell projection; Complete proteome; Cytoplasm; LIM domain; Membrane; Metal-binding; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Repeat; Transcription; Transcription regulation; Zinc. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 386 AA 
Protein Sequence
MEELDALLEE LERSTLQDSD EYSNPAPLPL DQHSRKETNL DETSEILSIQ DNTSPLPAQL 60
VYTTNIQELN VYSEAQEPKE SPPPSKTSAA AQLDELMAHL TEMQAKVAVR ADAGKKHLPD 120
KQDHKASLDS MLGGLEQELQ DLGIATVPKG HCASCQKPIA GKVIHALGQS WHPEHFVCTH 180
CKEEIGSSPF FERSGLAYCP NDYHQLFSPR CAYCAAPILD KVLTAMNQTW HPEHFFCSHC 240
GEVFGAEGFH EKDKKPYCRK DFLAMFSPKC GGCNRPVLEN YLSAMDTVWH PECFVCGDCF 300
TSFSTGSFFE LDGRPFCELH YHHRRGTLCH GCGQPITGRC ISAMGYKFHP EHFVCAFCLT 360
QLSKGIFREQ NDKTYCQPCF NKLFPL 386 
Gene Ontology
 GO:0042995; C:cell projection; IEA:UniProtKB-KW.
 GO:0005737; C:cytoplasm; IDA:UniProtKB.
 GO:0005925; C:focal adhesion; IDA:UniProtKB.
 GO:0005634; C:nucleus; IDA:UniProtKB.
 GO:0048471; C:perinuclear region of cytoplasm; IEA:UniProtKB-SubCell.
 GO:0005886; C:plasma membrane; IDA:UniProtKB.
 GO:0002102; C:podosome; IDA:UniProtKB.
 GO:0003712; F:transcription cofactor activity; IDA:UniProtKB.
 GO:0008270; F:zinc ion binding; IEA:InterPro.
 GO:0007155; P:cell adhesion; NAS:ProtInc.
 GO:0050859; P:negative regulation of B cell receptor signaling pathway; IDA:UniProtKB.
 GO:0007162; P:negative regulation of cell adhesion; IDA:UniProtKB.
 GO:0006461; P:protein complex assembly; TAS:ProtInc.
 GO:0033628; P:regulation of cell adhesion mediated by integrin; IEA:Compara.
 GO:0006355; P:regulation of transcription, DNA-dependent; IEA:UniProtKB-KW.
 GO:0007165; P:signal transduction; TAS:ProtInc.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 
Interpro
 IPR017305; Leupaxin.
 IPR001781; Znf_LIM. 
Pfam
 PF00412; LIM 
SMART
 SM00132; LIM 
PROSITE
 PS00478; LIM_DOMAIN_1
 PS50023; LIM_DOMAIN_2 
PRINTS