CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-038756
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
  
Protein Name
 Nesprin-2 
Protein Synonyms/Alias
  
Gene Name
 Syne2 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
1590DKINQICKNLQYHLNubiquitination[1]
1609FEDPPFEKEANAIVDubiquitination[1]
2979QLGNLRDKLAAIRGSacetylation[2]
5417DILPRALKDIKGLQSacetylation[3]
6186TQLELINKQYRRLARacetylation[4]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [2] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns.
 Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV.
 Cell Rep. 2012 Aug 30;2(2):419-31. [PMID: 22902405]
 [3] Quantification of mitochondrial acetylation dynamics highlights prominent sites of metabolic regulation.
 Still AJ, Floyd BJ, Hebert AS, Bingman CA, Carson JJ, Gunderson DR, Dolan BK, Grimsrud PA, Dittenhafer-Reed KE, Stapleton DS, Keller MP, Westphall MS, Denu JM, Attie AD, Coon JJ, Pagliarini DJ.
 J Biol Chem. 2013 Jul 17;. [PMID: 23864654]
 [4] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337
Functional Description
  
Sequence Annotation
  
Keyword
 Actin-binding; Complete proteome; Reference proteome; Repeat. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 6870 AA 
Protein Sequence
MAASPVLPTE DGEGFLGIDD LHFSLQAEQE DTQKKTFTCW INSQLAKHTP PSVVSDLFAD 60
IKKGHVLLDL LEVLSGQQLP RDKGSNTFQC RINIEHALTF LKNRSIKLIN IHVADIVEGN 120
PSIILGLIWT IILHFHIEKL AQTLSCDYNQ PSPEVVSVAA SSPTSSPPTK KCSKAQAQAR 180
WQWSAKKALL QWAQEQCARS ESVNVTDFKS SWRNGMAFLA VIHALRPDLI DMDSMRHRSN 240
KDNLKEAFRI AEHELKIPKL LEPEDVDVVN PDEKSIMTYV AQFLKYSKDA PGPGDSTQAK 300
VRDALVWLTL QEKRFQKMLK DSASETYCNK YHSLLSFMES LNEEKESFID VLSLKGRMGE 360
LNEDESRLRQ GWTSLMHQVA AWRAQLDDAL PSPLKETEAW LKDIEGVVQE GVPTSQSYSE 420
ARTLIQGKLS SFKSLMGSFD YHSDVLMAFQ SNAEKSLPAV PPVKLEEMTR RINNVLGKNF 480
IPLLEFHDSK CSVLALLDEA KAKLDVWNGT YESKESVEVL LEDWHKFTGE KKFLIQLDAS 540
FQKCEEMYKN SARECESIRE EYMMLEKNVH SCRQYIHNTK ATLQRALMSW ATFEEDLALL 600
KASFDLTKKE QIKEVPVETL LQWNTKHTSL NEVGSFLIGV SSREVAASIS KELRRLNKRW 660
RKFITKTPLE LKLPLVKIQD QPPGNSSGTS LSKESAMAAE PGGSRGEDVK AAEKQEVEDE 720
ESAGQLKVNE EVEGLIKQVT IWESQTKSIL DLLQHGDHAD GSSADTLQHL IAKGSVYEEL 780
LARTEDTLQM DVQSPSNLEP FQNVLRAGLQ AKIQEAKQGV QITMVELSAV LKNLSDEPLE 840
LDLGLKVEEA QKELEVSILR AEQLLGQRER PGGFLLKYKE ALEILNTNSL AKYLRAVEEL 900
KRTVPGGAKL QLEEQSRVAS AKWEPLRHEI SLYLQQLKIA IEEEKLRDNI ARLEKQINKE 960
KKLIRRGRTR GLRKEHEACL SPESIKCQLE HHVGVLRVLC EELTSPEDQQ ELKRALRDYE 1020
QKIARLLKCA SEIHTTLQSS QGGALEERSA LITTENGRRD ADGEVPLEIP DNQLSTEKAM 1080
EPIKNFSQTS ELKPQQEESI MEKEGKDCSA SLSDLQERYD TQRGLLEQHL QDSKSRVTSD 1140
FASEQERSSA CLQSKLAELQ VLLADTDAHW EKFEITSLNL RRLMSDAEKP VLNQERDLLK 1200
GNEQVLHGLL NTRMESLEMA LQIVLPLEKE CSLLCASDLP LCTVAVQDLH PVEIDGVYQN 1260
LRDIRDSIAK QIRVCTSLEE PSNSVPRELH TLDQCAIQDI VLKCRLQLET MNQKVEMRED 1320
ALDALEGFLA SLRAAKLSAE LPADRPAPKA PEVLSEDILL MKEKAGPLDE RLRTLGINIK 1380
DAEGGENTTC ERLVGALSVN LVAMDGQSKE EGPPEDKKLL EACSSKNLEL FKNIQDLQNQ 1440
ISKIGLKDPT APAVKHRKKS LLRLDKDLDG LEEEKVRIQK IAGSLPRFKD GSEKNVIQQC 1500
EDTAALWEST KASVTESLEQ CGSALELLRQ YQNIKNNLTA LIQKEEGIIS QQASYMGKDN 1560
LKKKIAEIET VKEEFSDHLE VVDKINQICK NLQYHLNKMK TFEDPPFEKE ANAIVDRWLD 1620
INEKTEEYGE NLGRALALWD KLFIIKNNID EWTEQILGKA ESHELTEEDR GRLKEELKVL 1680
EEQSAEFSRR VADIQSLLQS NEKPLELQVM ESSVLSKMKD VKTHVAGGSN SYAPSGSTAE 1740
LREDLDQAKT QMGMTESLLN ALSPSDSLEI FTKLEEIQQQ IFQQKHSMTV LENQIGCLTP 1800
ELSELKRQYA SVSNLFNTKK NALQDHFATF LNEQCKNFND WFSNVKTNLQ ECFEPPETKL 1860
SLEQRLQKLS DFLTLGGGNS KIQQVETVLQ HVKMLLPKAH VKELDSWLRS QELELENMES 1920
ICQARAGELN NSFQQLLRLE DDCRSLSKWL TNQEENWGKM EVSGERMDLF SQALTRKREQ 1980
FETVAQLSDS LKEHGLTEGE ETIKESTHLI DRYQALWRQL HEIEEEDKLP AAEDQSFNDL 2040
ADDVIHWIKE IKESLMALNS SEGKMPLEER IQKIKEIIAL KPEGDAKIQM VMRQAEHCEA 2100
PLAQETFTDL SNQWDSTLHL ANTYLSHQEK LVLEGEKYLQ SKEDLRLMLT ELKKQQEAGF 2160
ALQPGLPEKQ AQLKIYKKFL QKAQDLTSLL EELKSQGNYL LECTKNPSFS EEPWLEVKHL 2220
HESLLQQLQD SVQKLEGHVQ EHSSYQVCLT DLSSTLDDIS KEYFSLCDGS KDQIMAKERM 2280
QKLQELESRL RFQGGALKKA SALAKSIKQN TSSVGQKIIK DDIRSLKYKQ KDLENRIESA 2340
KQETENGLNS ILKSKSSTEK HVKFSLPVEE MPATSEVPKP TRESAAVGES GGARETNTNS 2400
AVEMILSKQL SLNVQESMQN AQDEREVNEL QNQPLELDIM LRNEQLKGME ELSTHLEARR 2460
AAIELLEQSQ HLNQTEEQAL VLPAARPSVC HLGSLQQELH TLKKTKERQY GLLSGFQDQL 2520
VMAEASLNTS LAEVESLKIG SLDSATYLGK IKKFLGSVEN QQGSLSKLRT EWAHLSSLLA 2580
AADQKLVESQ MKHLEHGWEL VEQLAHRKCF QQATEHSELT CLLEKLQDLK VSLHQQQQRL 2640
TLSLNSPGQQ AAIVDMVTPA AELQAIKCEF SGLKWQAELH MKRLWGEKDK KTLEDAINNL 2700
NKQMEALEPL NREVENRIKK CELQNRIKET LSWVKNTMAE LVVPIALLPD NILSQIRKCK 2760
LIHDGILGNQ QAVELLVEEV RGITPSLAPC EGDGLNALLE DLQSQHQALL LKSTERSQQL 2820
ELKLEEKSKL FAIIGKVQLT LEESETLMSP TGDRASTEAE LERRLAILKA SQQQLQDTES 2880
ALSAHLQELT NAYKDANVFE RLFLDDQLKN LKARTNRTQR FLQNNGSELK QKMESYREFH 2940
DKAAVLQKEA ECILHGGLLP LRQELEQDAK EQLGNLRDKL AAIRGSLSQV LTSEEVFDTI 3000
GLSWDGSLLA RLQTQVLERE REVEGKIKQL DTFLIARDRH QASISKIRAV DLQIKKGAES 3060
LLKVPSMSPE STLLNAQTLI QKIEKSKRLR DEIIRKLSKN EAFDDSFKES EMQRLKLCAE 3120
ENSRLQEALQ NMLLELQPRE MGEKEFREKL ENALHVLKQI QSRLQQPLCV NLGVQHIQHE 3180
KETWEAFGEQ VEAEMCGLRA VRITEEQREE NDSGTGGMEA KLRDIEGLHM ELSKSISLRA 3240
DVLNDAYDSA NRYDELVAGA LRIITSLEAT LLSYRVDLHN PQKTLELAHL KQEELQSSVA 3300
DLRSLTETLG AISSPEAKEQ LRCTLEVLAA KNSALKAGLE AQEAEEERCL ENYKCFRKMK 3360
EEICSRLRKM EMDLGQSIFP LPRSYKEALA RLEQSKALTS NLLSTKEDLV KLRQLLRHLR 3420
CRSTENDATC ALGVASALWE KWLSLLEAAR EWQQWGGELK REWKFISEEI EREAIILETL 3480
QEDLPEISKT NAAPTEELWQ LLDSLCQHQE SVEKQQLLLA LLLQRVRSIQ NIPEGTETGE 3540
TIPALQEIGS MQERCDRLLH TTRKNKDLVQ AEIQAQQSFL KEIKDVKRVF EQISTSFPNL 3600
APEGHPERAE QFEELRSILQ KGKLSFENIM EKLRIKYSEM YSIVPAEIGS QVEECRSALE 3660
DAEEKMSSEV SKSSPSSIMR RKIERINNGL HCVEKMLQQK SRNIEEAHEI QKKIWDELDL 3720
WHSKLNELDS EVQDFVEQDP GQAQEWMDNL MAPFQQHQQV SQRAESRTSQ LNKATIKMEE 3780
YNDLLKSTEV WIEKTSCLLA NPACYDSSRT LSHRASTLQM ALEDSEQKHS LLHSIFTDLE 3840
DLSIIFETDD LIQTIHELSD QVAALQQQIM EALPHVQQVA DDVVAIESEV KAMEKKVAKI 3900
KAILLSKEIF DFPPEEHLKH GEVILENIHP MKKTIAEIMT YQVELRLPQT GTKPLPVFQR 3960
TSQLLQDVKL LENVTQEQNE LLKVVIKQTA ECDEEIDSLK QMLTNYSAEI SPEHVSQNQV 4020
ADLPSLQGEM ERLEKQILNL NQKKEDLLVD LKTAVLNLHE HLKQEQQEVG DKPSAGASEC 4080
TVAERDASER KLSRTNSMSF LPVVKEEAEE SSVKSEDGRR RTEPPSASWS FLGKHSKDLE 4140
GDGASSSSSA TIVQDADGRI STCDSSMVHI IAPDSGSTEE GPAPSPRLSQ TDEGATPPIE 4200
AALLDFPREQ GAFESTVERS RPRPADILRV CKTQVAKLEL WLQQANVAFE PETVDADMQQ 4260
VVEEELAGCQ AMLTEIEYKV ASLLETCKDQ GLGDCGTTQQ EAEALSWKLK TVKCNLEKVQ 4320
MVLQEKFSED QHPSTLKKPS EPHDVDQPAG LSELDSVLTE RPQFSRQKDA PQPQILELKP 4380
SEQKDLIKFT ELNAKKTWLQ GHQENEDANR QSASSSKVPS PGNAASDSTL PLQAQSGDKW 4440
QYLHHELTSR PNPSVPQLVE PQVALTTSTL PSVSVYNFRC PTADELQAYT TQLEELRQEA 4500
NTIQTQGSMT EETYISLDKR LFELFLSLSR CLGSVEGLLQ RPGLLREDAC AQQVFFQKLA 4560
LELKKLYLAL GDKKDDFLKA VTWPGKEATL LPECIDALTV SLESVQSRAA WRDASLKAGL 4620
EHSRSYQNEV KRLYSQLIKK KTALQQSLNE ISGQSISKQL QKADVHTAEL QNSEKQVAKL 4680
RDEGERLRFP HGLLQDVYKL EDVLDSMWGI LRARYLELSS PFLSKSLQTL LQGMAELVSI 4740
GKGKLAADPL QHAKSKAALQ AQLQDHKAFF QKLVADMLLI QTYSATMFPP SLQKGEGFGA 4800
EQVAEVRALE EEACLRGAQL QSMLQKWEEF DDNYASLEKD LEALISSLPS VSLVEETEER 4860
LLERISFYQQ IKRNIDGKHA RLCQTLNEGR QLAASVSCPE PEGQIARLEE QWLSLNKRID 4920
QELHRLQTLL KHLLSYSRDS DELTRWLETS QQTLNYWKEQ SLNVSQDLNT IRSNIDRFFK 4980
FSKEVDERSS LKSAVMSTGN QLLHLKEADT ATLRASLAQF EQKWTVLITQ LPDIQEKLHQ 5040
LQMEKLPSRE AISEMISWMN AVEPQAAGKD TELSKSSASQ VKHLLQKLKE FRMEMDYKQW 5100
VVDFVNQSLL QLSTCDVESK RYERTEFAEH LGEMNRQWQR VHGTLNRKIQ HLEQLLESIT 5160
ENENKVQNLN SWLEAQEERL KMLQKPESAV SMEKLLLDCQ DIENQLALKS KALDELRQSS 5220
LTMDGGDVPL LEDMASGIVE LFQKKNNVTS QVHQLRASVQ SVLQEWKACD KLYDEATMRT 5280
TQLTYSMEHS KPAVLSLQAL ACQVQNLEAL QDEAENGERS WEKLQEVIGR LKASCPSMAG 5340
IIEEKCQDAH SRWTQVNQDL ADQLQEARGQ LQLWKAPHNA HAEAAAWLQQ QEAKFQQLAN 5400
TNLSGDNLAD ILPRALKDIK GLQSDLQKTK EAFLENSTLS DQLPQPEERS TPGLHSGQRH 5460
SLQTAAYLEK MLLAKSNEFE IVLAQFKDFT DRLAYSKDLI VHKEENLNKL YHEEKEEVPD 5520
LFLNHVLALT AQSPDIERLN EESLRLPLSD VTIKTLQSLN RQWIRATATA LDHYSELQGN 5580
GLNEKFLHYC ERWIQVLEKI QESLSVEVAH SLPALLEQQK TYEILEAEVS TNQAVADAYV 5640
TQSLQLLDTA EIEKRPEFVS EFSKLSDQWQ RAARGVRQRK CDISRLVTQW RFFTTSVEDL 5700
LRFLADTSQL LSAVKEQDCY SLCQTRRLVH ELKSKEIHLQ RWRTTYALAL EAGEKLRNTP 5760
SPETREFVDG QISRLQESWK DTELSLGEVI SRLQSTAETW DQCKKKIKKL KKRLQALKAQ 5820
SEDPLPELHE ALHEEKELIK EVEKSLANWT HSLKELQTMK ADLSQHILAE DVTVLKEQIQ 5880
LLHRQWEDLC LRVAIRKQEI EDRLNSWIVF NEKNKELCAW LVQMENKVLQ TADVSIEEMI 5940
EKLQKDCMEE ISLFTENKLQ LKQMGDQLIK ASSKAKAAEL EEKLSKINDR WQHLFDVIGS 6000
RVKKLKETFA FIQQLDKNMS NLRTWLARIE SELSKPVVYD VCDNQEIQKR LAEQQDLQRD 6060
IEQHSAGVES VFNICDVLLH DSDACANETE CDSIQQTTRS LDRRWRNICA MSMERRMKIE 6120
ETWRLWQKFL DDYSRFEDWL KSAERTAACP NSSEVLYTNA KEELKRFEAF QRQIHERLTQ 6180
LELINKQYRR LARENRTDTA SKLKQMVHEG NQRWDNLQKR VTAILRRLRY FTNQREEFEG 6240
TRESILVWLT EMDLQLTNVE HFSESDAEDK MRQLNGFQQE ITLNTNKIDQ LIVFGEQLIQ 6300
KSEPLDAVLI EDELEELHRY CQEVFGRVSR FHRRLTSHTP GLDDEKEASE NETDIEDPRE 6360
IQADSWRKRR ESEEPTSPQS LCHLVPPALG HERSGCETPV SVDSIPLEWD HTGDVGGSSS 6420
HEDDEEGPFY SALSGKSISE GHPWHVPDSP SHSKHHYKHM EGDRTEAPVP TDASTPFKSD 6480
YVKLLLRQGT DDSKEGLKEA QQEDEQLATL TGQQPGAFDR WELIQAQELH SKLRLKQTVQ 6540
QLKSDIGSIA AWLGKTEAEL EALKLAEPPS DIQEIALRVK RLQEILKAFD TYKALMVSVN 6600
VSHKEYLPSQ SPEATELQNR LHQLSLSWDS VQGVLDSWRG DLRQSLMQCQ DFHQLSQDLL 6660
LWLATAESRR QKAHVTSPEA DRQVLLECQK DLMRLEKELV ARQPQVSSLR EISSSLLVKG 6720
QGEDYIEAEE KVHVIEKKLK QLQEQVAQDL MSLQRSLDPD ASLTSFDEVD SGEQLPAAFA 6780
KQFGVEEEEE EEETDSRMPH LDSPGSSQPR RSFLSRVIRA ALPLQLLLLL LLLLACLLPA 6840
SEDDYSCTQA NNFARSFYPM LRYTNGPPPT 6870 
Gene Ontology
 GO:0016021; C:integral to membrane; IEA:InterPro.
 GO:0005635; C:nuclear envelope; IDA:MGI.
 GO:0007163; P:establishment or maintenance of cell polarity; IMP:MGI.
 GO:0010761; P:fibroblast migration; IMP:MGI.
 GO:0006998; P:nuclear envelope organization; IMP:MGI.
 GO:0034504; P:protein localization to nucleus; IMP:MGI. 
Interpro
 IPR001589; Actinin_actin-bd_CS.
 IPR001715; CH-domain.
 IPR012315; KASH.
 IPR018159; Spectrin/alpha-actinin.
 IPR002017; Spectrin_repeat. 
Pfam
 PF00307; CH
 PF10541; KASH
 PF00435; Spectrin 
SMART
 SM00033; CH
 SM00150; SPEC 
PROSITE
 PS00019; ACTININ_1
 PS00020; ACTININ_2
 PS50021; CH
 PS51049; KASH 
PRINTS